TGF-β-DPC-4 mediated cell cycle regulation

TGF-β-DPC-4 介导的细胞周期调节

• 批准号: 09470042
• 负责人: AKIYAMA Tetsu
• 金额: $ 8.45万
• 依托单位: The University of Tokyo (1998)Osaka University (1997)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470042/
• 关键词: DPC4; TGF-β; Target gene; One-hybrid; Subtraction; Cell Cycle; DPC-4,; TGF-b,; TAK1,; TAB-1,; 増殖制御、; 細胞周期、; シグナル伝達、; 転写制御

DPC4 (SMAD4) forms a complex with SMAD 2 /SMAD3, and induces transcriptional activation of TGF-beta target genes. We attempted to isolate target genes by yeast one-hybrid system using DPC4 as bait. We screened 2 x 105 clones of a human genome library and isolated three fragments that contain the consensus sequence recognized by DPC4. We searched for the DDBJ, EMBL and GenBank databases and found that one clone is a fragment of the human 28S rRNA gene. We are also trying to isolate DPC4 target genes involved in the regulation of cell cycle progression and signal transduction by the subtraction method and the promoter trap method. To understand the mechanism underlying the DPC4-mediated cell cycle regulation, we are also trying to identify novel DPC4-associated proteins.

DPC 4（SMAD 4）与SMAD 2 /SMAD 3形成复合物，并诱导TGF-β靶基因的转录激活。我们尝试以DPC 4为诱饵，利用酵母单杂交系统分离目的基因。我们筛选了人类基因组文库的2 × 105个克隆，并分离出含有DPC 4识别的共有序列的三个片段。我们检索了DDBJ、EMBL和GenBank数据库，发现一个克隆是人28 S rRNA基因的片段。我们还尝试通过消减法和启动子捕获法分离DPC 4中参与细胞周期进程调控和信号转导的靶基因。为了了解DPC 4介导的细胞周期调控的机制，我们还试图鉴定新的DPC 4相关蛋白。

项目成果

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