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Detection of RNA editing and reconstruction of plant molecular phylogenetic tree

RNA编辑检测与植物分子系统发育树重建

基本信息

批准号：
09304076
负责人：
YOSHINAGA Koichi
金额：
$ 21.31万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

1, RNA editing in hornwortsTranscripts from more than 20 genes were edited in the chloroplast of Anthoceros formosae. Number of editing sites were 20 to 4 per 1000 bases, and no silent editing was observed. The sites of cytidine (C) to uridine (U) conversion were found at almost the same amount as those of U to C conversion. Every editing site was determined by guide RNA (gRNA) which located in the same RNA.In the mitochondria of A. formosae, 30 editing sites were observed in 420 bases region of cox1 transcript. The conversion of U to C was also observed.2, RNA editing in liverwortsNo RNA editing was found in the mitochondrial cox1 transcript from 4 kinds of liverworts.3, RNA editing in lycopsidsExtensive RNA editing was found in atpB transcripts in the chloroplasts from 4 kinds of lycopsids, which included U to C conversion. Sequences corresponding to gRNA were also found in the same RNA. In the mitochondria from Lycopodium, 5 sites of RNA editing were found in the region of 420 nucleotides of cox1 transcript. They were C to U conversion.4, RNA editing in fernsAbout 90 sites of RNA editing were found in the transcripts of 14 genes in the chloroplasts from Pteridium. The conversion of U to C was also found in addition of C to U. However, no editing was expected through out the entire transcripts of chloroplast genome from Psilotum.In conclusion, existence of RNA editing was not strictly related with the evolution of green plant. Phylogenetic relationship based in the nucleotide sequence of chloroplast genome was not altered even when the nucleotide sequence was collected by RNA editing.

1、犀角植物的RNA编辑在犀角植物（Anthoceros formosae）叶绿体中编辑了20多个基因的转录本。每1000个碱基中编辑位点数为20 ~ 4个，未观察到沉默编辑。胞苷(C)转化为尿苷(U)的位点与U转化为C的位点几乎相同。每个编辑位点都是由位于同一RNA中的引导RNA （gRNA）确定的。在台湾沙蚕线粒体中，cox1转录物的420个碱基区中发现了30个编辑位点。同时还观察到U向C的转化。2、肝脏的RNA编辑4种肝脏线粒体cox1转录物均未发现RNA编辑。3、番茄红素中的RNA编辑4种番茄红素叶绿体中atpB转录本存在大量的RNA编辑，包括U到C的转化。在相同的RNA中也发现了与gRNA对应的序列。在石蒜线粒体中，在420个cox1转录物核苷酸区发现了5个RNA编辑位点。它们是C到U的转换。4、蕨类植物的RNA编辑在蕨类植物叶绿体的14个基因转录本中发现了约90个RNA编辑位点。除了C到U的转化外，还发现U到C的转化。然而，未发现对裸菇叶绿体基因组的整个转录本进行编辑。综上所述，RNA编辑的存在与绿色植物的进化没有严格的关系。基于叶绿体基因组核苷酸序列的系统发育关系即使通过RNA编辑收集核苷酸序列也未发生改变。