STRUCTURAL CHARACTERIZATION OF A CHLOROPLAST RIBOSOME

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 高等植物叶绿体含有70 S样核糖体，根据它们的细菌祖先。 然而，这些生物体负担着来自多个基因组（核、叶绿体、线粒体）的基因表达的协调。 此外，叶绿体中基因表达的控制主要是在转录后完成的。 我们感兴趣的是叶绿体核糖体的蛋白质组学和结构表征，因为这将有助于我们理解这个细胞器中存在的翻译控制的独特机制。 没有一个细胞器核糖体的结构数据曾被公布。 然而，蛋白质组学分析已公布从多个叶绿体核糖体揭示保守的蛋白质独特的标准细菌核糖体。 藻类如莱茵衣藻的叶绿体被靶向用于大规模蛋白质表达。 为了优化该生物体中各种异源蛋白的表达水平，我们需要更好地理解工作中的翻译调节机制。 叶绿体核糖体的结构特征将是这一理解的一个组成部分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Higher plant chloroplasts contain 70S-like ribosomes, as per their bacterial ancestry. However, these organisms are burdened with the coordination of gene expression from multiple genomes (nuclear, chloroplast, mitochondrial). Additionally, control of gene expression in the chloroplast is accomplished primarily post-transcriptionally. We are interested in a proteomic and structural characterization of the chloroplast ribosome, as this will aid in our understanding of the unique mechanisms of translation control present in this organelle. No structural data from an organelle ribosome has ever been published. However, proteomic analyses have been published from multiple chloroplast ribosomes revealing conserved proteins unique from a standard bacterial ribosome. The chloroplasts of algae like Chlamydomonas reinhardtii are being targeted for large scale protein expression. In order to optimize expression levels of a variety of heterologous proteins in this organism we need to better understand the mechanisms of translation regulation at work. Structural characterization of the chloroplast ribosome will be an integral part of this understanding.