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Involvement of annexin 5 in G protein mediated signal transduction

膜联蛋白 5 参与 G 蛋白介导的信号转导

基本信息

批准号：
14360181
负责人：
KAWAMINAMI Mitsumori
金额：
$ 7.62万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14360181/
关键词：
G protein annexin 5 GnRH LH βsubunit pituitary gonadotrope corpus luteum testis プロラクチン

项目摘要

A new G protein mediated signal transduction mechanism was studied. It was clarified that annexin 5 took part in the G protein coupled signal transduction process. Annexin is a member of newly identified calcium-phospholipid binding proteins. The outline of the results is brought together as follows.1.From GnRH receptor to annexin 5 synthesisIt was clarified that the MAPK system lay between the GnRH receptor and annexin 5 mRNA synthesis in the pituitary gonadotropes. In addition, it was observed that annexin 5 was indispensable to the process that GnRH promotes the gonadotrophin secretion, and clarified that annexin 5 was a new signal factor of the GnRH receptor signal transduction.2.Annexin 5 in the ovaryChanges in ovarian annexin 5 were analyzed. The synthesis of annexin 5 was demonstrated to start after luteinization and the expression of annexin 5 was inhibited by prolactin. It was clarified that annexin 5 appeared in corpus luteum by the inhibition of prolactin secretion and those cells were in apoptosis. In addition, it was clarified that local GnRH enhanced annexin 5 expression and induced apoptosis.3.Annexin 5 in the testisAnnexin 5 was demonstrated to distribute to various testicular cells. Among these, endotherial cells and Leydig dells were intensely stained with anti-annexin 5. It was clarified that annexin 5 of Leydig cells was increased by the administration of human chorinic gonadotropin. This effect was shown to be mediated by the local GnRH action.Getting together, annexin 5 was shown to be under the control of GnRH receptor in the anterior pituitary gonadotropes, luteal cells and Leydig cells. Annexin 5 is a biomarker of GnRH receptor. GnRH receptor-annexin 5 system seems to be utilized in various cell species.

研究了一种新的G蛋白介导的信号转导机制。明确了膜联蛋白5参与了G蛋白偶联信号转导过程。膜联蛋白是新发现的钙磷脂结合蛋白的一员。结果概要如下。从GnRH受体到膜联蛋白5的合成明确了MAPK系统在垂体促性腺激素中GnRH受体和膜联蛋白5 mRNA合成之间的作用。此外，观察到膜联蛋白5在GnRH促进促性腺激素分泌的过程中不可或缺，明确了膜联蛋白5是GnRH受体信号转导的新信号因子2。卵巢膜联蛋白5分析卵巢膜联蛋白5的变化。膜联蛋白5的合成在黄体化后开始，泌乳素抑制膜联蛋白5的表达。阐明了膜联蛋白5是通过抑制泌乳素分泌而在黄体中出现的，其细胞处于凋亡状态。此外，明确了局部GnRH可增强膜联蛋白5的表达并诱导细胞凋亡。睾丸中的膜联蛋白5已被证实分布于各种睾丸细胞。其中，内皮细胞和间质谷被抗膜联蛋白5强烈染色。结果表明，人绒毛膜促性腺激素可增加间质细胞的膜联蛋白5。这种作用被证明是由局部GnRH作用介导的。综上所述，在垂体前叶促性腺细胞、黄体细胞和间质细胞中，膜联蛋白5受GnRH受体的控制。膜联蛋白5是GnRH受体的生物标志物。GnRH受体-膜联蛋白5系统似乎在各种细胞中都有应用。

项目成果

期刊论文数量（48）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Chromosomal mapping and zygosity check of transgenes based on flanking genome sequences determined by genomic walking

DOI：
10.1538/expanim.53.103
发表时间：
2004-04-01
期刊：
EXPERIMENTAL ANIMALS
影响因子：
2.4
作者：
Noguchi, A;Takekawa, N;Suzuki, O
通讯作者：
Suzuki, O

Prolactin inhibits annexin 5 expression and apoptosis in the corpus luteum of pseudopregnant rets : Involvement of local gonadotropin-releasing hormone

催乳素抑制假孕rets黄体中膜联蛋白5的表达和细胞凋亡：局部促性腺激素释放激素的参与

DOI：
发表时间：
2003
期刊：
Endocrinology 144(8)
影响因子：
0
作者：
Takahashi H;Takahashi M;Nagaya H;Hirako M;Sawai K;Minamihashi A;Inumaru S;Yokomizo Y;Geshi M;Okano A;Okuda K;Kawaminami M
通讯作者：
Kawaminami M

Regulatory sequence elements of mouse GLUT4 gene expression in adipose tissues