Development of advanced purification system using high performance specific microorganisms for water enviromment restoration

本研究的重点是如何制造特定微生物的孢子，并明确了实际应用的方法。因此。发展了特定微生物的群体培养方法。本研究选取了硝化细菌（Nitrobacter属和Nitorosomonas属）、弯曲原生动物（Protozoa Colpidium campylum）、梨形四膜虫（Tetrahymena Pyriformis）、小口Vorticella microstoma convallaria)、红眼轮虫（Rotifer Philodina erythrophthalma）、旋转轮虫（rottaria rotatoria）、Lecane luna、Oljgochaeta Aeolosoma hemprichi、Nais variabils、Pristina longiseta等特定微生物。考察了不同环境因素对这些特定微生物的影响，了解了这些特定微生物对水温、PH、磷酸盐缓冲液浓度等不同环境因素的反应。溶解氧浓度、食物质量和数量、震动压力等。这些结果让我们知道了大规模培养这些特定微生物的最佳方法。此外，通过模型生态系统调查，阐明了这些特定微生物与污水处理设施中常见的各种微生物之间的关系。另一方面，研究了这些特定微生物的最佳产孢方法。阐明了这些孢子的萌发方法。此外。聚乙二醇(PEG)。比较了聚乙烯醇（PVA）和纤维素作为实际使用的质量比微生物保鲜剂的物理强度、比表面积和活性保持比。获得了特种微生物利用先进技术的基本情况。

The focal point of this research is how to make spore of specific microorganisms, and clarify the practical use methods. Thus. the methods of mass culture about specific microorganisms were developed. Some specific microorganisms such as nitrification bacteria (Genus Nitrobacter and Genus Nitorosomonas), Protozoa Colpidium campylum, Tetrahymena Pyriformis, Vorticella microstoma, and Vorticella convallaria, Rotifer Philodina erythrophthalma, Rotaria rotatoria, Lecane luna, Oljgochaeta Aeolosoma hemprichi, Nais variabils, Pristina longiseta and so on were selected in this study. The effects of some environmental factors on these specific microorganisms were tested to know the response of these specific microorganisms against various kind of environmental factors such as water temperature, PH, phosphate buffer concentration. dissolved oxygen concentration qualities and quantities of food, shake stress and so forth. These results let us know the best way for mass cultivation of these specific microorganisms. Furthermore, the relationship among these specific microorganisms and various kind of common microorganisms that appearing in wastewater treatment facilities through model ecosystem investigations was clarified. On the other hand the best method to make spore of these specific microorganisms was studied. The germination method from these spores was clarified. In addition. polyethylene glycol(PEG). polyvinyl alcohol(PVA) and cellose were compared as mass specific microorganisms keeper in practical use for analysis of physical strength, specific surface area, activity maintaining ratio. The basic information about advaced technology on utilization of specific microorganisms was obtained.