Studies on development of new method for water quality assessment using microcosm system

微观系统水质评价新方法开发研究

• 批准号: 04650505
• 负责人: INAMORI Yuhei
• 金额: $ 1.28万
• 依托单位: NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04650505/
• 关键词: Microcosm; Environmental assessment; Ecosystem assessment; Surfactant; Chemical pesticide; Genetically engineered microorganism; Water quality assessment; Harmful chemical matter; 遺伝子組替え微生物; 微生物農薬

Tne aim of this study is to establish a new method for water quality assessment with the experimental examination about the development of an aquatic microcosm system as one of model ecosystems which consist of bacteria as decomposer, algae as producer, and protozoa and micro metazoa as consumer, and which shows very high reproductivity and reflectivity of natural eco-system, and about the prosperity and decay of these microbiota with addition of surfactant such as detargent, chemical pesticide, and/or genetically engineered microorganisms such as micribial pesticide. This year, environmental assessment of these matters using microcosm system was attempted.In the case of surfactant addition such as LAS, soap and composit soap, it was made clear that the effective concentration to the microbiota of micricism system was lower that that of EC_<50> which was employed usually, and a possibility of analysis of gas balance in matter cycle with closed micricism system was showrd.In the case of … More chemical pesticide addition such as symadine and benziocurb, it was made clear that the effect to micribiota was different by the sort of pesticide, and that herbicide has no effect with the concentration of the environmental standard, and that micricism system is able to be one of essential method to decide the ranking of effectiveness to ecosystem.In the case of genetically engineered microorganism addition such as microbial pesticide, it was made clear that predation of protozoa and micro metazoa very affected to the prosperity and decay of genetically engineered microorganisms, and the pattern of prosperity and decay of these bacteria in microcosm system was controled by the combinetion of host bacterial strain and plasmid DNA.From these results obtained from this study, it was made clear that our aquatic microcosm system is expected to be one of a valid method for environmental assessment, and that more correct assessment will be possible with the combination of monoxenic culture test, flask-size microcosm test and natural lake model ecosystem as tier test method. Less

本研究的目的是建立一种新的水质评价方法，并对一个以细菌为分解者，藻类为生产者，原生动物和微型后生动物为消费者的水生微宇宙系统的发展进行实验研究，该系统具有很高的自然生态系统的繁殖力和反射率。以及这些微生物群在添加表面活性剂如去垢剂、化学杀虫剂和/或基因工程微生物如微生物杀虫剂的情况下的繁荣和衰退。今年，我们尝试了用微宇宙系统对这些物质进行环境评价。在添加表面活性剂如LAS、肥皂和复合皂的情况下，发现微宇宙系统对微生物群的有效浓度低于通常使用的EC_2<50>，并显示了用封闭的微宇宙系统分析物质循环中气体平衡的可能性。在添加表面活性剂如LAS、肥皂和复合皂的情况下， ...更多信息 通过对化学农药西玛因、灭幼脲等的添加研究，表明不同农药对微生物区系的影响不同，除草剂对环境标准浓度的影响不明显，微生物系统可作为判定其对生态系统有效性强弱的重要方法之一，而在微生物农药等基因工程微生物添加的情况下，研究表明，原生动物和微型后生动物的捕食作用对基因工程菌的繁荣有很大的影响，微生物在微宇宙系统中的繁荣模式是由宿主菌株和质粒DNA的结合所控制的。表明我们水生微宇宙系统有望成为环境评价的有效方法之一，以烧瓶微宇宙试验和天然湖泊模型生态系统为层次试验方法。少

项目成果

Y.Inamori: "Effect of Microbial Pesticide on Growth of Micro Animals" Japanese Journal of Water Treatment Biology. Vol.29. 31-38 (1993)

Y.Inamori：“微生物农药对微生物生长的影响”日本水处理生物学杂志。

Yuhei Inamori: "Prosperity and Decay of Microbial Pesticide,Bacillus thuringiensis in Aquatic Model Ecosystems Environmental Assessment Method" Water sience and Technology. (1994)

稻盛和夫：“水生模型生态系统中微生物农药、苏云金芽孢杆菌的繁荣与衰落环境评价方法”水科学与技术。

"マイクロコズムにおける添加組換え微生物の生残に及ぼす代謝産物の影響" 日本水処理生物学会誌. 別巻12号. 89-89 (1992)

“代谢物对微观世界中添加的重组微生物存活的影响”日本水处理生物学会杂志增刊第 12 卷 89-89（1992 年）。

Y.Inamori: "Prosperity and Decay of Microbial Pesticide, Bacillus thuringiensis in Aquatic Model Ecosystems for Environmental Assessment Method" Water Science and Technology. (in press).

Y.Inamori：“环境评价方法水生模型生态系统中微生物农药、苏云金芽孢杆菌的繁荣与衰落”水科学与技术。

稲森 悠平: "マイクロコズムにおける微生物農薬BT菌の生残と温度との関係" 日本水処理生物学会誌. 別巻12号. 87-87 (1992)

Yuhei Inamori：“微生物杀虫剂 BT 细菌的存活与微观世界温度的关系”日本水处理生物学会杂志增刊第 12 期 87-87（1992 年）。