Study of serological and genetical diagnoses for B virus infection in monkeys

猴B病毒感染的血清学和遗传学诊断研究

• 批准号: 09400014
• 负责人: SATO Hiroshi
• 金额: --
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09400014/
• 关键词: B virus; Monkey; Virus infection; Japanese monkey; Cynomolgus monkey; Rhesus monkey; ヘルペスウイルス

1. A serological investigation by means of an enzyme immuno assay for herpes B virus (Cercopithecine herpesvirus 1) was performed on 962 sera of healthy nonhuman primates reared in laboratory animal facilities which belong to Japanese Association of Laboratory Animal Facility of National University. An antibody prevalence of 40% (384/961) was demonstrated. The antibody titer was shown to be higher among macaques (60% of cynomolgus monkeys, 53% of rhesus monkeys, and 34% of Japanese monkeys) than among non-macaque species (21%). These data were also alarmed the use of Japanese monkeys in a research field of life science.2. We examined the use of alternative or substitutive antigen for immuno assay method instead of dangerous B virus ; 1) human herpes virus 1 (HSV1), 2) herpesvirus papio 2 (HVP2) antigen. The correlationship between HSV1 (gP and the gD antigen) and B virus ELISA was 96%. On the other hand, the correlationship between HVP2 antigen and B virus ELISA was of 98% using 350 monkey serum samples from USA and Japan. These were suggestive data that alternative method may use for the antibody assay, especially HVP2 antigen.3. It was possible to detect B virus genome in 3 samples in the antibody positive 21 sample (trigeminal ganglion) of Japanese monkeys which are originated from Japan, after trying the RT-nested PCR method.4. It accomplished in using two kinds of glycoprotein antigens of BV as the recombinant antigen. However, their products showed cytotoxic effect to mammal cells and therefore judged impractical.

1. 对日本国立大学实验动物协会实验室饲养的962只健康非人灵长类动物血清进行了B型疱疹病毒（Cercopithecine herpesvirus 1）的酶免疫检测。抗体阳性率为40%（384/961）。结果显示，猕猴（食蟹猴60%，恒河猴53%，日本猴34%）的抗体滴度高于非猕猴物种（21%）。这些数据也警示了在生命科学研究领域使用日本猴子的行为。探讨了用替代抗原或替代抗原代替危险的B病毒进行免疫测定的方法；1)人类疱疹病毒1型（HSV1）， 2)疱疹病毒2型（HVP2）抗原。HSV1 （gP和gD抗原）与B病毒ELISA的相关性为96%。另一方面，美国和日本的350份猴子血清HVP2抗原与B病毒ELISA检测结果的相关性为98%。这些数据提示可采用其他方法进行抗体测定，特别是HVP2抗原测定。3 .采用rt -巢式PCR方法，在日本猕猴抗体阳性的21份样本（三叉神经节）中检测到3份乙型病毒基因组。完成了用两种BV糖蛋白抗原作为重组抗原的研究。然而，他们的产品显示出对哺乳动物细胞的细胞毒性，因此被认为是不切实际的。

项目成果

H.Sato et al: "Prevalence of Herpes B virus Antibody among Nonhuma Primates reared in the National University of Japan" Experimental Animal. 1998 ((in press))

H.Sato等人：“日本国立大学饲养的非胡马灵长类动物中B型疱疹病毒抗体的流行”实验动物。

K,Ohsawa: "Herpesvirus popio2:Alternative antigen for use in monkey B virus diagnostic assays."Laboratory Animal Science. 49. 605-616 (1999)

K,Ohsawa：“疱疹病毒 popio2：用于猴 B 病毒诊断测定的替代抗原。”实验动物科学。