Study of serological and genetical diagnoses for B virus infection in monkeys

1.采用酶免疫分析法对国立大学日本实验动物设施协会饲养的962份健康非人灵长类动物血清进行了疱疹B病毒（1型猕猴疱疹病毒）的血清学调查。抗体流行率为40%（384/961）。猕猴（60%食蟹猴、53%恒河猴和34%日本猴）的抗体滴度高于非猕猴种属（21%）。这些数据也震惊了日本猴子在生命科学研究领域的使用。我们研究了用替代抗原代替危险的B病毒作为免疫检测方法的抗原：1）人类疱疹病毒1型（HSV 1），2）猴疱疹病毒2型（HVP 2）。HSV 1（gP和gD抗原）与B病毒ELISA的相关性为96%。用美国和日本的350份猴血清进行检测，HVP 2抗原与B病毒ELISA的相关性为98%。这些数据提示，替代方法可用于抗体测定，特别是HVP 2抗原。对21份抗体阳性的日本猴三叉神经节标本进行RT-nested PCR检测，结果在3份标本中检出B病毒基因组.成功地将BV的两种糖蛋白抗原作为重组抗原。然而，他们的产品显示出对哺乳动物细胞的细胞毒性作用，因此被认为是不切实际的。

1. A serological investigation by means of an enzyme immuno assay for herpes B virus (Cercopithecine herpesvirus 1) was performed on 962 sera of healthy nonhuman primates reared in laboratory animal facilities which belong to Japanese Association of Laboratory Animal Facility of National University. An antibody prevalence of 40% (384/961) was demonstrated. The antibody titer was shown to be higher among macaques (60% of cynomolgus monkeys, 53% of rhesus monkeys, and 34% of Japanese monkeys) than among non-macaque species (21%). These data were also alarmed the use of Japanese monkeys in a research field of life science.2. We examined the use of alternative or substitutive antigen for immuno assay method instead of dangerous B virus ; 1) human herpes virus 1 (HSV1), 2) herpesvirus papio 2 (HVP2) antigen. The correlationship between HSV1 (gP and the gD antigen) and B virus ELISA was 96%. On the other hand, the correlationship between HVP2 antigen and B virus ELISA was of 98% using 350 monkey serum samples from USA and Japan. These were suggestive data that alternative method may use for the antibody assay, especially HVP2 antigen.3. It was possible to detect B virus genome in 3 samples in the antibody positive 21 sample (trigeminal ganglion) of Japanese monkeys which are originated from Japan, after trying the RT-nested PCR method.4. It accomplished in using two kinds of glycoprotein antigens of BV as the recombinant antigen. However, their products showed cytotoxic effect to mammal cells and therefore judged impractical.