Molecular Basis for RNA Dynamic Function

RNA动态功能的分子基础

• 批准号: 09278101
• 负责人: WATANABE Kimitsuna
• 金额: $ 144.7万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09278101/
• 关键词: RNA Catalytic Function; RNA Information Function; RNA High-level Function; Rybozyme; Translation Factors; Aplicing; Mitochondria; tRNA; 触媒機能; 情報機能; 高次機能; RNAの触媒機能; RNA動的機能; mRNAの発現調節; 分子擬態; 高次生命現象; RNA結合蛋白質; RNAの高次構造; ミトコンドリア病; 動的構造の解析法

The aim of this research project is to establish the molecular basis of RNA dynamic functions in the phenomena of life by systematizing the RNA dynamic functions through revealing the molecular mechanisms of catalytic functions of RNA and high-level control functions of information expression, and searching and artificially producing ribozymes catalyzing new reaetions. On the basis of the results obtained by these researches, molecular mechanisms of high-level phenomena of life such as development, differentiation and disease will be pursued and many unknown RNA functions will be elucidated. In parallel with these achievements new methods for analyzing RNA dynamic functions will be developed. For this purpose three research groups and one special team were organized under a master group. The first, 「RNA dynamic functions」 group succeeded in determination of a minimal functional region of group I intron possessing the splicing function, construction of dimeric hammerhead ribozyme (maxiz … More yme) providing both sequence recognition and RNA scission activities, and expression of the complex of the maxizyme with a helicase in cells. The second, 「RNA information fuctions」group obtained a highly appreciated result of discovery of tRNA molecular mimicry by translation factors」 which leads to elucidating the basic principle of the translation reaction. In research for RNA recognition mechanisms by proteins, NMR analysis of a complex of sxl protein with a single-stranded RNA and X-ray analysis of a complex of aminoacyl-tRNA synthetase with tRNA succeeded in elucidating molecular recognition mechanisms of RNA by proteins. The third, 「RNA high-level functions」 succeeded in constructing a mitochondrial gene-introduced mouse useful for elucidating the cause of disease and mechanism of senescence. It was found that mitochondrial diseases are caused by lacking in the taurine-modification at the anticodon first position of mitochondrial tRNAs. The elucidation of functional structures of tmRNA was also successful. The research project has been progressing well and it is estimated that about 75% of the original aims have been achieved. During progress in the research project Japan RNA Society, a supporting system for supporting the RNA research in Japan was established on the basis of the project. Less

The aim of this research project is to establish the molecular basis of RNA dynamic functions in the phenomena of life by systematizing the RNA dynamic functions through revealing the molecular mechanisms of catalytic functions of RNA and high-level control functions of information expression, and searching and artificially producing ribozymes catalyzing new reaetions. On the basis of the results obtained by these researches, molecular mechanisms of high-level phenomena of life such as development, differentiation and disease will be pursued and many unknown RNA functions will be elucidated. In parallel with these achievements new methods for analyzing RNA dynamic functions will be developed. For this purpose three research groups and one special team were organized under a master group. The first, 「RNA dynamic functions」 group succeeded in determination of a minimal functional region of group I intron possessing the splicing function, construction of dimeric hammerhead ribozyme (maxiz … More yme) providing both sequence recognition and RNA scission activities, and expression of the complex of the maxizyme with a helicase in cells. The second, 「RNA information fuctions」group obtained a highly appreciated result of discovery of tRNA molecular mimicry by translation factors」 which leads to elucidating the basic principle of the translation reaction. In research for RNA recognition mechanisms by proteins, NMR analysis of a complex of sxl protein with a single-stranded RNA and X-ray analysis of a complex of aminoacyl-tRNA synthetase with tRNA succeeded in elucidating molecular recognition mechanisms of RNA by proteins. The third, 「RNA high-level functions」 succeeded in constructing a mitochondrial gene-introduced mouse useful for elucidating the cause of disease and mechanism of senescence. It was found that mitochondrial diseases are caused by lacking in the taurine-modification at the anticodon first position of mitochondrial tRNAs. The elucidation of functional structures of tmRNA was also successful. The research project has been progressing well and it is estimated that about 75% of the original aims have been achieved. During progress in the research project Japan RNA Society, a supporting system for supporting the RNA research in Japan was established on the basis of the project. Less

项目成果

渡辺, 中村, 井上他: "志村、渡辺共編「RNA研究の最前線」"シュプリングフェアラーク東京. 238 (2000)

Watanabe、Nakamura、Inoue 等人：“Shimura 和 Watanabe 共同编辑，“RNA 研究的前线”，Spring Verlag Tokyo。238 (2000)

Ikawa, Y., Inoue, T. et al.: "Minimal catalytic domain of a group I self-splicing intron RNA"Nature Str. Biol.. 7. 1032-1035 (2000)

Ikawa, Y., Inoue, T. 等：“I 组自剪接内含子 RNA 的最小催化结构域”Nature Str。

吉村, 渡辺 共編: "RNA 研究の最前線"スプリングフェアラーク東京. 238 (2000)

Yoshimura 和 Watanabe 共同编辑：“RNA 研究的前沿”Spring Fairark 东京 238 (2000)。

Ito, S. et al.: "Functional integrity of mt genomes in human platelets and actopsied brain tissues"Proc. Natl. Acad. Sci. USA. 96. 2099-2103 (1999)

Ito, S. 等人：“人类血小板和脑组织中 mt 基因组的功能完整性”Proc。

Nitta,I.et al: "A novel cell-free System for Peptide synthesis driven by pyridine" Biol.Chem.379. 819-829 (1998)

Nitta,I.et al：“一种由吡啶驱动的新型无细胞肽合成系统”Biol.Chem.379。