Development of High Performance DNA Sequencer Using Laser-Induced Capillary Vibration Method

利用激光诱导毛细管振动法开发高性能 DNA 测序仪

基本信息

  • 批准号:
    05558083
  • 负责人:
  • 金额:
    $ 12.16万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1995
  • 项目状态:
    已结题

项目摘要

As can be seen from Human Genome Project, acceleration of analysis time and improvement of sensitivity will promote both fundamental and application study. In recent years, capillary zone electrophoresis (CZE) has attracted much attention as a separation tool for ultramacro amounts of components. however, CZE has no detection method sensitive enough for ultramicro amounts of separated componts, and it has been difficult to apply to ultramicro amounts of materials such as DNA fragments. We proposed capilary vibration induced by laser (CVL) method as a highly sensitive on-column detector for CZE,and attomole amounts of DNA fragments were quantitatively detected using the CVL method. In this study we aimed at developing a novel DNA sequencing method with higher separation efficiency and sensitivity by one order of magnitude by maans of analytical chemistry and molecular biology.This year is the last year of the three year research. In the previous year, we developed pulsed laser-induced stationary wave CVL detection method. We applied the developed method to detection of DNA fragments, and the detection limit of absolute amount was 16 amol. The method was also applied to gene diagnosis, and DNA fragments which was considered to have relation with heart disease or Alzheimer's disease were sensitively detected. These results suggested feasibility of development of gene diagnosis, which is a key to preventive medicine and early detection. Moreover, we applied to high performance immune diagnosis, we investigated on simple method for separating immunologically bound samples and free samples, and developed more sensitive and simpler immunoassay method than the conventional method. Attomole amounts of biological materials were detected with the same or higher sensitivity than the methods using fluorescent tags or radioisotopes. Realization of ultrasensitive detection without labeling process will lead to open a new application field in DNA analysis.
从人类基因组计划可以看出,分析时间的加快和灵敏度的提高将促进基础研究和应用研究。近年来,毛细管区带电泳(CZE)作为一种超大量组分的分离工具受到了广泛关注。然而,CZE对于超微量的分离组分没有足够灵敏的检测方法,并且它难以应用于超微量的材料,例如DNA片段。提出了一种高灵敏度的毛细管激光诱导振动(CVL)柱上检测器,并对阿摩尔量的DNA片段进行了定量检测。本研究的目的是利用分析化学和分子生物学的方法,开发一种分离效率和灵敏度都提高一个数量级的DNA测序新方法,今年是本研究的最后一年。在前一年,我们开发了脉冲激光诱导驻波CVL检测方法。将该方法应用于DNA片段的检测,绝对量检测限为16阿莫尔。该方法也被应用于基因诊断,并敏感地检测出被认为与心脏病或阿尔茨海默病有关的DNA片段。这些结果表明,基因诊断的发展是可行的,这是一个关键的预防医学和早期发现。此外,我们还将其应用于高效免疫诊断,研究了分离免疫结合样品和游离样品的简单方法,开发了比常规方法更灵敏、更简单的免疫分析方法。与使用荧光标记或放射性同位素的方法相比,以相同或更高的灵敏度检测生物材料的阿托摩尔量。实现无标记的超灵敏检测将为DNA分析开辟一个新的应用领域。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Tamao Odake, Takehiko Kitamori, Tsuguo Sawada: "Photothermal Effects in a Capillary and Optimum Conditions for Absorbance Detection of Capillary Electrophoresis." Analytical Chemistry. 67. 1458-1463 (1995)
Tamao Odake、Takehiko Kitamori、Ttsuguo Sawada:“毛细管中的光热效应和毛细管电泳吸光度检测的最佳条件。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
T.Odake,et al.: "Ultratrace Detection of Capillary Gel Electrophoresis by Laser-Induced Capillary Vibration" Analytical Chemistry. (発表予定). (1994)
T.Odake 等人:“激光诱导毛细管振动的毛细管凝胶电泳的超微量检测”分析化学(即将发表)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Tamao Odakc, Takchiko Kitamori, Tsugno Sawada: "Photothermal Effects in a Capillary and Optimum Conditions for Absorbance Detection of Capillary Elcctrophoresis." Analytical Chemistry. 67. 1458-1463 (1995)
Tamao Odakc、Takchiko Kitamori、Tsugno Sawada:“毛细管中的光热效应和毛细管电泳吸光度检测的最佳条件。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
M.Harada,et al.: "Effect of Probe Beam Offset on Quantitative Measurement in Photothermal Beam Diflection Spectroscopy" Analytical Chemistry. 65. 2181-2183 (1993)
M.Harada 等人:“探测光束偏移对光热光束偏转光谱定量测量的影响”分析化学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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WATANABE Kimitsuna其他文献

WATANABE Kimitsuna的其他文献

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{{ truncateString('WATANABE Kimitsuna', 18)}}的其他基金

Molecular mechanisms of co-evolution of mitochondrial genetic codes and tRNA in the evolution of animals
动物进化中线粒体遗传密码与tRNA共同进化的分子机制
  • 批准号:
    21570242
  • 财政年份:
    2009
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Intermolecular Network specific for mitochondrial translation systems and its functional characteristics
线粒体翻译系统特有的分子间网络及其功能特性
  • 批准号:
    14035206
  • 财政年份:
    2002
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Elucidation of basic principle in genetic information translation system by using the specialty of animal mitochondria
利用动物线粒体的特性阐明遗传信息翻译系统的基本原理
  • 批准号:
    14208077
  • 财政年份:
    2002
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Elucidation of the construction principle of the mitochondria translation system by means of structural biology
用结构生物学阐明线粒体翻译系统的构建原理
  • 批准号:
    11308024
  • 财政年份:
    1999
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Analysis of functional structure of ribosomal RNA
核糖体RNA功能结构分析
  • 批准号:
    10044196
  • 财政年份:
    1998
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular Basis for RNA Dynamic Function
RNA动态功能的分子基础
  • 批准号:
    09278101
  • 财政年份:
    1997
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas (A)
A Novel Cell-Free Peptide Synthesis Driven by Pyridine
吡啶驱动的新型无细胞肽合成
  • 批准号:
    08555200
  • 财政年份:
    1996
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular mechanism of the animal mitochondrial translation systems
动物线粒体翻译系统的分子机制
  • 批准号:
    08408025
  • 财政年份:
    1996
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular mechanism of protein synthesis
蛋白质合成的分子机制
  • 批准号:
    07044183
  • 财政年份:
    1995
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Mechanism of RNA function expression
RNA功能表达机制
  • 批准号:
    04272102
  • 财政年份:
    1992
  • 资助金额:
    $ 12.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas

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具有液相色谱 (LC) 和毛细管电泳 (CE) 功能的高分辨率质谱仪
  • 批准号:
    515835726
  • 财政年份:
    2023
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  • 批准号:
    10759354
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    2023
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Planar Capillary Electrophoresis
平面毛细管电泳
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New Advances in Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry for Accelerating Biomarker Discovery in Metabolomics
多段注射-毛细管电泳-质谱法加速代谢组学生物标志物发现的新进展
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SBIR 第二阶段:用于生物制药和生物医学应用的下一代毛细管电泳和质谱分析
  • 批准号:
    2025299
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    $ 12.16万
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    Cooperative Agreement
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脂质体封装预浓缩后水中纳米颗粒和纳米材料上环境毒性和生化活性污染物毛细管电泳分析解吸技术的发展
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New Advances in Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry for Accelerating Biomarker Discovery in Metabolomics
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