JOINT RESEARCH ON IN VIVO ESR IMAGING AND SPECTROSCOPY

体内 ESR 成像和光谱学联合研究

• 批准号: 09044197
• 负责人: OHYA Hiroaki
• 金额: $ 1.86万
• 依托单位: Yamagata Public Corporation For the Development Of Industry, Institute Life Support Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044197/
• 关键词: ESR; IN VIVO; IMAGING; SPECTROSCOPY; SPIN PROBE; SEARCH COIL; SPATIOTEMPORAL MEASUREMENT; スピンプローブ; サーケコイル; インビボESR; 分光法; Lバンド; ラジカル; LODESR

ESR spectroscopy has been a unique method detecting and identifying in vivo free radicals, and tracing their physiological functions. However, this method has not been responding urgent requirements from biological sciences including medicine and physiology. We have so far investigated some ESR imaging methods for small animals and succeeded to construct low-frequency equipments picturing in vivo distributions of the free radicals in small animals.In this project we aimed to survey the spin-probe reagents suitable for the equipments which were developed by the Dartmouth medical group, and in addition we tried to investigate a methodology developing in vivo ESR imaging and ESR spectroscopy for diagnosis of human diseases. Actually the samples developed in each group were exchanged each other and introduced to suitable research systems. Typical results are as follows : (1) Novel spin probes detecting reactive oxygen species(ROS) generated in biological cells were succeeded to synthesize, (2) Methods introducing the probes to the body of small animals and picturing the distribution of ROS generated in vivo were established, (3) A standard spin probe detecting in vivo oxygen concentration and its time dependence was supplied for the estimation of both sensitivities of ESR equipments used to compare each other, and there was no fundamental difference of the sensitivities between both equipments, and (4) A search-coil type ESR method developed in both laboratories for rat organisms were shown to be an efficient in vivo diagnosis method estimating the effects of drags and food supplements. These results are essential to develop advanced ESR imaging and spectroscopy in our institute and expected to be widely applied for in vivo biological systems.

ESR波谱技术是一种独特的检测和鉴定体内自由基并追踪其生理功能的方法。然而，这种方法已经不能满足医学和生理学等生物科学的迫切需要。我们已经研究了一些小动物的ESR成像方法，并成功地构建了小动物体内自由基分布的低频成像装置，本项目的目的是考察适合于达特茅斯医学组开发的装置的自旋探针试剂，此外，我们还试图研究一种用于诊断人类疾病的体内ESR成像和ESR波谱学方法。实际上，每个小组开发的样品相互交换，并介绍给合适的研究系统。典型结果如下：（1）成功合成了检测生物细胞中活性氧（ROS）的新型自旋探针。（2）建立了将探针导入小动物体内并拍摄体内ROS分布的方法。（三）为评价ESR设备的灵敏度提供了一种标准的自旋探针，用于检测体内氧浓度及其时间依赖性（4）两个实验室开发的用于大鼠生物体的搜索线圈型ESR方法被证明是一种有效的体内诊断方法，可以估计药物和食物补充剂的影响。这些结果对我们研究所发展先进的ESR成像和光谱学是必不可少的，并有望广泛应用于在体生物系统。

项目成果

Hidekatsu Yokoyama: "In vivo longitudinally detected ESR measurements at microwave regions of 380,700,and 900 MHZ in rats treated with a nitroxide radical" J.Mag.Reson.129. 201-206 (1997)

Hidekatsu Yokoyama：“在用硝基氧自由基处理的大鼠中，在 380,700 和 900 MHZ 微波区域体内纵向检测 ESR 测量”J.Mag.Reson.129。

H.Kamada, H.Ohya: "Proceedings of hre 2nd Intern.Conf.on Bioradicals" Yamagata Technopolis Foundation, 350 (1998)

H.Kamada、H.Ohya：“第二届生物自由基实习生会议记录”山形科技城基金会，350 (1998)

Hidekatsu Yokoyama: "A CT using longitudinally detected ESR (LODESR-CT)of intraperitoneally injected nitroxide radical in a rat's head" Magnetic Resonance Imaging. 15.6. 701-708 (1997)

Hidekatsu Yokoyama：“使用纵向检测大鼠头部腹腔注射硝基氧自由基的 ESR (LODESR-CT) 进行 CT”磁共振成像。

T.Igarashi et al: "New sensitive agents detecting singlet oxygen by ESR spectroscopy" Free Radical Biology and Medicine. 24 (in print). (1999)

T.Igarashi 等人：“通过 ESR 光谱检测单线态氧的新型敏感剂”自由基生物学和医学。

M.Aoyama et al: "A novel ESR method for HRP activity using a combination of p-AP and hydroxylamine, and its application to enzyme immunoassays" Analytical Science. 14. 1107-1113 (1998)

M.Aoyama 等人：“使用 p-AP 和羟胺组合测定 HRP 活性的新型 ESR 方法及其在酶免疫测定中的应用”《分析科学》。