Development of transgenic animals for the study of feeding behavior and stress response

开发用于研究摄食行为和应激反应的转基因动物

• 批准号: 09044307
• 负责人: INUI Akio
• 金额: $ 4.1万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044307/
• 关键词: feeding behavior; transgenic mouse; knockout mouse; neuropeptide; neuropeptide Y; 神経ペクチド; Neuropeptide Y

Recently novel molecular mediators and regulatory pathways for feeding and body weight regulation have been identified in the brain and the periphery. Mice overexpressing or lacking these mediators have been produced by gene targeting, and observations on mutant mice have shed new light on the role of each element in the homeostatic loop of body weight regulation. We have developed neuropeptide Y overexpressing mouse which exhibited anxiety-like behavior (heteromouse) and obese tendency (homomouse). We have also developed pancreatic polypeptide overex-pressing mouse which exhibited thin phenotype associated with decreased food intake and gastric emptying rates. These are examples that were produced in support of this research grant.

最近，新的分子介质和喂养和体重调节的调节途径已被确定在大脑和外周。小鼠过度表达或缺乏这些介质已产生的基因靶向，和观察突变小鼠有新的光的作用，每个元素在体内平衡循环的体重调节。我们已经开发了神经肽Y过表达小鼠，表现出焦虑样行为（异鼠）和肥胖倾向（同鼠）。我们还开发了胰腺多肽过表达小鼠，其表现出与食物摄入和胃排空率降低相关的瘦表型。这些都是为了支持这项研究补助金而制作的例子。

项目成果

Burrows HL et al.: "Excess CRH Binding Protein in the Hypothalamic-Pituitary-Adrenal Axis in Transgenic mice." J.Clin Invest.(in press). (1998)

Burrows HL 等人：“转基因小鼠的下丘脑-垂体-肾上腺轴中存在过量的 CRH 结合蛋白。”

A.Inui et al.: "Anxiety-like Behavior in Transgenic Mice with Brain Expression of Neuropeptide Y." Proceedings of the Association of American Physicians. 110. 171-182 (1998)

A.Inui 等人：“大脑表达神经肽 Y 的转基因小鼠的焦虑样行为”。

M.Okita et al.: "Effects of corticotropin-releasing factor on feeding and pancreatic polypeptide response in the dog." J.Endocrinol.156. 359-364 (1998)

M.Okita 等人：“促肾上腺皮质激素释放因子对狗的进食和胰多肽反应的影响。”

Burrows HL et al.: "Excess CRH Binding Protein in the Hypothalamic-Pituitary-Adrenal Axis in Transgenic Mice." J.Clin.Invest.101. 1439-1447 (1998)

Burrows HL 等人：“转基因小鼠的下丘脑-垂体-肾上腺轴中存在过量的 CRH 结合蛋白。”

M.Okita et al.: "Effects of corticotropin-releasing factor on feeding and pancreatic polypeptide response in the dog." J.Endocvinal.156. 359-364 (1998)

M.Okita 等人：“促肾上腺皮质激素释放因子对狗的进食和胰多肽反应的影响。”