ROLE OF TG RICH RIPOPROTEINS IN ATHEROSCLEROSIS

富含 TG 的 RIPO 蛋白在动脉粥样硬化中的作用

• 批准号: 10044249
• 负责人: TANAKA Akira
• 金额: $ 13.25万
• 依托单位: TOKYO MEDICAL AND DENTAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044249/
• 关键词: ApoB48 receptor; Postprandialhyperlipidemia; Atherosclerosis; TG rich lipoprotein; Immunohistochemical stain; RLP cholesterol; Remnant; Insulin resistance; coronary artery disease; 脂肪負荷試験; アポB48レセプター; RLP

Unique receptor, apo B48 receptor, was identified in human monocyte-macrophages. The receptor binds and internizes triglyceride(TG)rich Lipoproteins via a domain in apo B48. The apo B48 receptor cDNA sequences were elucidated. Immunohistochemical studies indicate colocalization of anti-apo B48 receptor Ab and monocyte-macrophage specific Ab in human atherosclerotic lesion foam cells. This suggests that apo B48 receptor may contribute to foam cell formation and atherosclerosis in abnormal state with elevated TG-rich lipoproteins.Apo B100 expression in the epithelial cells of small intestine were identified by immunoblotting and dot-blotting of PCR-amplified cDNA.It suggests that dietary VLDL may be synthesized in human small intestine epithelium and converted into LDL, atherogenic lipoprotein.Some cinical studies showed that increase of remnants(RLP cholesterol), especially in postprandial state, has an important role in developimg atherosclerosis.Remnants(RLP cholesterol)were indicated to increases in the state of insulin resistance.

在人单核巨噬细胞中发现了独特的受体apo B48受体。该受体通过载脂蛋白B48的结构域结合并内化富含甘油三酯（TG）的脂蛋白。鉴定了载脂蛋白B48受体cDNA序列。免疫组织化学研究表明，抗载脂蛋白B48受体Ab和单核巨噬细胞特异性Ab在人动脉粥样硬化病变泡沫细胞中共定位。提示载脂蛋白B48受体可能参与泡沫细胞形成和动脉粥样硬化异常状态下富tg脂蛋白升高。采用免疫印迹法和pcr扩增cDNA斑点印迹法检测Apo B100在小肠上皮细胞中的表达。提示膳食VLDL可能在人小肠上皮内合成并转化为致动脉粥样硬化脂蛋白LDL。一些临床研究表明，残体（RLP胆固醇）的增加，特别是在餐后状态下，在动脉粥样硬化的发生中起重要作用。残馀物（RLP胆固醇）显示在胰岛素抵抗状态下增加。

项目成果

Bradley WA, Gianturco SH, et al: "Antipeptide antibodies reveal interrelationship of MBP200 and MBP235: unique apo B-48 receptors for lipoproteins on human monocyte-macrophages"J Lipid Res.. 40. 744-752 (1999)

Bradley WA、Gianturco SH 等人：“抗肽抗体揭示了 MBP200 和 MBP235 的相互关系：人单核细胞巨噬细胞上脂蛋白的独特 apo B-48 受体”J Lipid Res.. 40. 744-752 (1999)

Abbasi F, Tanaka A, Reaven GM, et al: "Fasting remnant lipoprotein cholesterol and triglyceride concentrations are evaluated in non-diabetic, insulin resistent, female volunteers"J Chin. Endcrinol. Metab. 84. 3903-3906 (1999)

Abbasi F、Tanaka A、Reaven GM 等人：“在非糖尿病、胰岛素抵抗女性志愿者中评估空腹残余脂蛋白胆固醇和甘油三酯浓度”J Chin。

Marcoux C, Cohn JS, et al: "Characterization of remnant-like particles isolated by immunoaffinity gel from the plasma of type III hyperlipoproteinemic patients"J Lipid Res. 40. 636-647 (1999)

Marcoux C、Cohn JS 等人：“通过免疫亲和凝胶从 III 型高脂蛋白血症患者血浆中分离出的残留样颗粒的表征”J Lipid Res。

Akio Kawakami,Akira Tanaka, et al: "Role of Remnant lipoproteins in Atherosclerosis."Ann.N.Y.Acad.Sci.. 902. 352-356 (2000)

Akio Kawakami、Akira Tanaka 等人：“残余脂蛋白在动脉粥样硬化中的作用。”Ann.N.Y.Acad.Sci.. 902. 352-356 (2000)

Abbasi F,Tanaka A,Reaven GM, et al: "Fasting remnant lipoprotein cholesterol and triglyceride concentrations are evaluated in non-diabetic, insulin resistent, female volunteers"J Clin.Endcrinol.Metab. 84. 3903-3906 (1999)

Abbasi F、Tanaka A、Reaven GM 等人：“在非糖尿病、胰岛素抵抗女性志愿者中评估空腹残余脂蛋白胆固醇和甘油三酯浓度”J Clin.Endcrinol.Metab。