ROLE OF TG RICH RIPOPROTEINS IN ATHEROSCLEROSIS

在人单核细胞-巨噬细胞中鉴定出独特的受体apo B48受体。该受体通过载脂蛋白B48中的结构域结合并内化富含甘油三酯（TG）的脂蛋白。对载脂蛋白B48受体cDNA序列进行了分析。免疫组织化学研究表明抗载脂蛋白B48受体抗体和单核巨噬细胞特异性抗体在人动脉粥样硬化病变泡沫细胞中共定位。通过免疫印迹和PCR扩增的cDNA斑点印迹技术证实了人小肠上皮细胞中ApoB 100的表达，提示人小肠上皮细胞可合成VLDL，并将其转化为LDL，从而使VLDL在小肠上皮细胞中的表达增加。一些临床研究表明，残余物（RLP胆固醇）的增加，特别是在餐后状态，在动脉粥样硬化的发展中起重要作用，残余物（RLP胆固醇）被认为是胰岛素抵抗状态的增加。

Unique receptor, apo B48 receptor, was identified in human monocyte-macrophages. The receptor binds and internizes triglyceride(TG)rich Lipoproteins via a domain in apo B48. The apo B48 receptor cDNA sequences were elucidated. Immunohistochemical studies indicate colocalization of anti-apo B48 receptor Ab and monocyte-macrophage specific Ab in human atherosclerotic lesion foam cells. This suggests that apo B48 receptor may contribute to foam cell formation and atherosclerosis in abnormal state with elevated TG-rich lipoproteins.Apo B100 expression in the epithelial cells of small intestine were identified by immunoblotting and dot-blotting of PCR-amplified cDNA.It suggests that dietary VLDL may be synthesized in human small intestine epithelium and converted into LDL, atherogenic lipoprotein.Some cinical studies showed that increase of remnants(RLP cholesterol), especially in postprandial state, has an important role in developimg atherosclerosis.Remnants(RLP cholesterol)were indicated to increases in the state of insulin resistance.