ROLE OF TG RICH RIPOPROTEINS IN ATHEROSCLEROSIS

在人单核巨噬细胞中发现了独特的受体apo B48受体。该受体通过载脂蛋白B48的结构域结合并内化富含甘油三酯（TG）的脂蛋白。鉴定了载脂蛋白B48受体cDNA序列。免疫组织化学研究表明，抗载脂蛋白B48受体Ab和单核巨噬细胞特异性Ab在人动脉粥样硬化病变泡沫细胞中共定位。提示载脂蛋白B48受体可能参与泡沫细胞形成和动脉粥样硬化异常状态下富tg脂蛋白升高。采用免疫印迹法和pcr扩增cDNA斑点印迹法检测Apo B100在小肠上皮细胞中的表达。提示膳食VLDL可能在人小肠上皮内合成并转化为致动脉粥样硬化脂蛋白LDL。一些临床研究表明，残体（RLP胆固醇）的增加，特别是在餐后状态下，在动脉粥样硬化的发生中起重要作用。残馀物（RLP胆固醇）显示在胰岛素抵抗状态下增加。

Unique receptor, apo B48 receptor, was identified in human monocyte-macrophages. The receptor binds and internizes triglyceride(TG)rich Lipoproteins via a domain in apo B48. The apo B48 receptor cDNA sequences were elucidated. Immunohistochemical studies indicate colocalization of anti-apo B48 receptor Ab and monocyte-macrophage specific Ab in human atherosclerotic lesion foam cells. This suggests that apo B48 receptor may contribute to foam cell formation and atherosclerosis in abnormal state with elevated TG-rich lipoproteins.Apo B100 expression in the epithelial cells of small intestine were identified by immunoblotting and dot-blotting of PCR-amplified cDNA.It suggests that dietary VLDL may be synthesized in human small intestine epithelium and converted into LDL, atherogenic lipoprotein.Some cinical studies showed that increase of remnants(RLP cholesterol), especially in postprandial state, has an important role in developimg atherosclerosis.Remnants(RLP cholesterol)were indicated to increases in the state of insulin resistance.