STUDY ON THE BIOORGANIC CHEMISTRY OF THE ANTITUMOR ANNONACEOUS ACETOGENINS IN CHINESE ANNONACEAE

中国番荔枝科抗肿瘤番荔枝苷的生物有机化学研究

• 批准号: 10660117
• 负责人: TANAKA Akira
• 金额: $ 2.24万
• 依托单位: COLLEGE OF TECHNOLOGY, TOYAMA PREFECTURAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660117/
• 关键词: Annonaceae; Annonaceous acetogenins; Brine shrimp test; Antibacterial activity; Plant growth inhibitory activity; Cyclohexene derivatives; バンレイシ科アセトゲニン; 殺ブラインシュリンプ活性

Antitumor annonaceous acetogenins in three chinese Annonaceae plants, Fissistigma oldhamii, Uvaria purpurea and Annona squamosa were investigated. Timbers or leaves of each plant were extracted with methanol and the extracts were concentrated to give oily residues. Repeated fractionations of these crude materials resulted in four to five fractions. In all experimental processes, the Kedde reagent and brine shrimp test were used to monitor the presence of annonaceous acetogenins. The former reagent can detect α,β-unsaturated γ-lactone moiety characteristic of annonaceous acetogenins. The brine shrimp test is known as a preliminary means for detecting antitumor substances. Unfortunately, we were not able to see any positive sign of the presence of annonaceous acetogenins-like substances in the above three chinese plants. However, we isolated two cyclohexene derivatives, zeylenol and zeylenone, of which the latter compound proved to be unknown and exhibited antibacterial and plant growth inhibitory activities.

研究了三种中国番荔枝科植物 Fissistigma oldhamii、Uvaria purpurea 和 Annona squamosa 中的番荔枝苷元的抗肿瘤作用。用甲醇提取每种植物的木材或叶子，并将提取物浓缩，得到油状残余物。这些粗原料的重复分馏产生了四到五个馏分。在所有实验过程中，均使用Kedde试剂和丰年虾试验来监测番荔枝苷的存在。前一种试剂可以检测番荔枝苷的α,β-不饱和γ-内酯部分特征。丰年虾试验被认为是检测抗肿瘤物质的初步手段。不幸的是，我们在上述三种中国植物中没有看到任何存在番荔枝苷类物质的积极迹象。然而，我们分离出两种环己烯衍生物，zeylenol和zeylenone，其中后者被证明是未知的，并表现出抗菌和植物生长抑制活性。