STUDY ON THE BIOORGANIC CHEMISTRY OF THE ANTITUMOR ANNONACEOUS ACETOGENINS IN CHINESE ANNONACEAE

本文研究了3种中国番荔枝科植物--紫花番荔枝、紫薇和番荔枝的抗癌活性。每种植物的木材或叶子都用甲醇提取，萃取物浓缩后得到油性残留物。这些原料的重复分馏产生了四到五个馏分。在所有的实验过程中，使用Kedde试剂和盐水虾试验来监测番荔枝内酯的存在。该试剂可检测番荔枝内酯的α，β-不饱和γ-内酯部分特征。盐水虾试验被认为是检测抗肿瘤物质的一种初步手段。不幸的是，我们没有看到任何积极的迹象表明，在上述三种中国植物中存在番荔枝内酯类物质。然而，我们分离出了两个环己烯衍生物，泽苯酚和玉米酮，后者的化合物被证明是未知的，并具有抗菌和植物生长抑制活性。

Antitumor annonaceous acetogenins in three chinese Annonaceae plants, Fissistigma oldhamii, Uvaria purpurea and Annona squamosa were investigated. Timbers or leaves of each plant were extracted with methanol and the extracts were concentrated to give oily residues. Repeated fractionations of these crude materials resulted in four to five fractions. In all experimental processes, the Kedde reagent and brine shrimp test were used to monitor the presence of annonaceous acetogenins. The former reagent can detect α,β-unsaturated γ-lactone moiety characteristic of annonaceous acetogenins. The brine shrimp test is known as a preliminary means for detecting antitumor substances. Unfortunately, we were not able to see any positive sign of the presence of annonaceous acetogenins-like substances in the above three chinese plants. However, we isolated two cyclohexene derivatives, zeylenol and zeylenone, of which the latter compound proved to be unknown and exhibited antibacterial and plant growth inhibitory activities.