STUDY ON THE BIOORGANIC CHEMISTRY OF THE ANTITUMOR ANNONACEOUS ACETOGENINS IN CHINESE ANNONACEAE

研究了三种中国番荔枝科植物——裂花、紫花花和番荔枝的抗肿瘤乙酰素。每种植物的木材或叶子用甲醇提取，提取物浓缩后得到油质残留物。这些原料的反复分馏得到四到五个馏分。在所有的实验过程中，采用kede试剂和盐水对虾试验来监测氨基乙酸原的存在。前一种试剂能检测出多糖的α，β-不饱和γ-内酯部分。盐水虾试验被称为检测抗肿瘤物质的初步手段。不幸的是，在上述三种中国植物中，我们没有看到任何积极的迹象表明存在类似无性系的乙酰原物质。然而，我们分离到了两个环己烯衍生物，zeylenol和zeylenone，后者被证明是未知的，并显示出抗菌和植物生长抑制活性。

Antitumor annonaceous acetogenins in three chinese Annonaceae plants, Fissistigma oldhamii, Uvaria purpurea and Annona squamosa were investigated. Timbers or leaves of each plant were extracted with methanol and the extracts were concentrated to give oily residues. Repeated fractionations of these crude materials resulted in four to five fractions. In all experimental processes, the Kedde reagent and brine shrimp test were used to monitor the presence of annonaceous acetogenins. The former reagent can detect α,β-unsaturated γ-lactone moiety characteristic of annonaceous acetogenins. The brine shrimp test is known as a preliminary means for detecting antitumor substances. Unfortunately, we were not able to see any positive sign of the presence of annonaceous acetogenins-like substances in the above three chinese plants. However, we isolated two cyclohexene derivatives, zeylenol and zeylenone, of which the latter compound proved to be unknown and exhibited antibacterial and plant growth inhibitory activities.