Visualising molecular level detail in single cells and intact tissues

可视化单细胞和完整组织的分子水平细节

• 批准号: DP210102148
• 负责人: Prof Hugh Harris
• 金额: $ 29.84万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210102148
• 关键词: Visualising; molecular; level; detail; single

The goal of this project is to deliver a new toolkit for imaging cells at an unprecedented resolution and level of chemical detail. We will expand the capabilities of two existing, but complementary, methods: optical fluorescence microscopy with responsive probes and X-ray fluorescence imaging. Expected outcomes include improved techniques and benchmarks for visualising bacterial and mammalian cells; development of new molecules for elucidating cellular chemistry; better utilisation of valuable synchrotron resources; and greater understanding of the strengths and limitations of current microscopy workflows. Results should benefit the biotechnology sector, and may lead to improved medical, diagnostic, and bioremediation capacity.

该项目的目标是提供一个新的工具包，用于以前所未有的分辨率和化学细节水平对细胞进行成像。我们将扩大两个现有的，但互补的方法的能力：光学荧光显微镜与响应探针和X射线荧光成像。预期成果包括改进的技术和基准可视化细菌和哺乳动物细胞;开发新的分子阐明细胞化学;更好地利用宝贵的同步加速器资源;以及更好地了解当前显微镜工作流程的优势和局限性。研究结果应该有利于生物技术部门，并可能导致医疗，诊断和生物修复能力的提高。