Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

摘要 我们的目标是极大地扩展我们现有的两个R01(肌动蛋白和核蛋白)的研究 复合体)，利用电子冷冻显微镜的进展 这是最近才用直接电子探测器实现的。此外，我们还将致力于 在许多项目上，这些项目不在目前R01的范围内，但涉及细菌 致病机制与人类健康密切相关：IV型菌毛，II型分泌物 系统，细菌和古细菌鞭毛细丝。通过研究许多不同的聚合物，我们 希望开发出更通用的方法，可以被其他人应用到螺旋聚合物中 生物学。由于存在大量这样的聚合物，从细胞骨架细丝到 对于螺旋状病毒(如埃博拉)，这项工作的潜在意义是巨大的 并有可能产生巨大的影响。

Abstract We aim to greatly extend our existing studies in the two current R01s (Actin and Nucleoprotein Complexes) in the proposed period, capitalizing on the advances in electron cryo-microscopy that have only recently become possible with direct electron detectors. In addition, we will work on a number of projects that are outside the scope of the present R01s, but involve bacterial pathogenesis and thus have great relevance to human health: Type IV pili, Type II Secretion System, bacterial and archaeal flagellar filaments. By working on many disparate polymers, we expect to develop more general methods that can be applied by others to helical polymers in biology. Since large numbers of such polymers exist, ranging from cytoskeletal filaments to amyloid to helical viruses (such as Ebola), the potential significance of this work is enormous and likely to have a great impact.