Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜

• 批准号: 10153815
• 负责人: EDWARD H. EGELMAN
• 金额: $ 67.54万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10153815
• 关键词: Acids; Actins; Amyloid; Bacteria; Biology; Biotechnology; Cholera; Complex; Cryoelectron Microscopy; Cytoskeletal Filaments; Ebola; Electrons; Filament; Health; Human; Meningitis; Methods; Muscle; Nucleoproteins; Organism; Pathogenesis; Pilum; Polymers; Proteins; Resolution; Structure; Type II Secretion System Pathway; Virus; Work; detector; tool

Abstract We aim to greatly extend our existing studies in the two current R01s (Actin and Nucleoprotein Complexes) in the proposed period, capitalizing on the advances in electron cryo-microscopy that have only recently become possible with direct electron detectors. In addition, we will work on a number of projects that are outside the scope of the present R01s, but involve bacterial pathogenesis and thus have great relevance to human health: Type IV pili, Type II Secretion System, bacterial and archaeal flagellar filaments. By working on many disparate polymers, we expect to develop more general methods that can be applied by others to helical polymers in biology. Since large numbers of such polymers exist, ranging from cytoskeletal filaments to amyloid to helical viruses (such as Ebola), the potential significance of this work is enormous and likely to have a great impact.

摘要 我们的目标是大大扩展我们现有的研究，在目前的两个R01（肌动蛋白和核蛋白 配合物）在拟议的时期，利用电子冷冻显微镜的进步， 直到最近才通过直接电子探测器实现。此外，我们将努力 一些项目超出了目前R01的范围，但涉及细菌 发病机制，从而有很大的关系，人类健康：IV型皮利，II型分泌 系统，细菌和古细菌鞭毛丝。通过研究许多不同的聚合物， 我希望开发出更通用的方法，可以应用于其他螺旋聚合物， 生物学由于存在大量这样的聚合物，从细胞骨架丝到 从淀粉样蛋白到螺旋病毒（如埃博拉病毒），这项工作的潜在意义是巨大的。 并且可能会产生巨大的影响。