Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜

• 批准号: 10153815
• 负责人: EDWARD H. EGELMAN
• 金额: $ 67.54万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10153815
• 关键词: Acids; Actins; Amyloid; Bacteria; Biology; Biotechnology; Cholera; Complex; Cryoelectron Microscopy; Cytoskeletal Filaments; Ebola; Electrons; Filament; Health; Human; Meningitis; Methods; Muscle; Nucleoproteins; Organism; Pathogenesis; Pilum; Polymers; Proteins; Resolution; Structure; Type II Secretion System Pathway; Virus; Work; detector; tool

Abstract We aim to greatly extend our existing studies in the two current R01s (Actin and Nucleoprotein Complexes) in the proposed period, capitalizing on the advances in electron cryo-microscopy that have only recently become possible with direct electron detectors. In addition, we will work on a number of projects that are outside the scope of the present R01s, but involve bacterial pathogenesis and thus have great relevance to human health: Type IV pili, Type II Secretion System, bacterial and archaeal flagellar filaments. By working on many disparate polymers, we expect to develop more general methods that can be applied by others to helical polymers in biology. Since large numbers of such polymers exist, ranging from cytoskeletal filaments to amyloid to helical viruses (such as Ebola), the potential significance of this work is enormous and likely to have a great impact.

抽象的 我们的目标是大大扩展我们对当前两个 R01（肌动蛋白和核蛋白）的现有研究 配合物）在拟议的时期内，利用电子冷冻显微镜的进步 最近才通过直接电子探测器才成为可能。此外，我们还将努力 一些项目超出了当前 R01 的范围，但涉及细菌 发病机制，因此与人类健康有很大关系：IV 型菌毛、II 型分泌物 系统、细菌和古细菌鞭毛丝。通过研究许多不同的聚合物，我们 期望开发出更通用的方法，可以被其他人应用于螺旋聚合物 生物学。由于存在大量此类聚合物，从细胞骨架丝到 淀粉样蛋白到螺旋病毒（如埃博拉病毒），这项工作的潜在意义是巨大的 并且很可能会产生很大的影响。