Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜

• 批准号: 10153815
• 负责人: EDWARD H. EGELMAN
• 金额: $ 67.54万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10153815
• 关键词: Acids; Actins; Amyloid; Bacteria; Biology; Biotechnology; Cholera; Complex; Cryoelectron Microscopy; Cytoskeletal Filaments; Ebola; Electrons; Filament; Health; Human; Meningitis; Methods; Muscle; Nucleoproteins; Organism; Pathogenesis; Pilum; Polymers; Proteins; Resolution; Structure; Type II Secretion System Pathway; Virus; Work; detector; tool

Abstract We aim to greatly extend our existing studies in the two current R01s (Actin and Nucleoprotein Complexes) in the proposed period, capitalizing on the advances in electron cryo-microscopy that have only recently become possible with direct electron detectors. In addition, we will work on a number of projects that are outside the scope of the present R01s, but involve bacterial pathogenesis and thus have great relevance to human health: Type IV pili, Type II Secretion System, bacterial and archaeal flagellar filaments. By working on many disparate polymers, we expect to develop more general methods that can be applied by others to helical polymers in biology. Since large numbers of such polymers exist, ranging from cytoskeletal filaments to amyloid to helical viruses (such as Ebola), the potential significance of this work is enormous and likely to have a great impact.

摘要