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Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution
近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜
批准号:
10793162
负责人:
EDWARD H. EGELMAN
金额:
$2.3万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
未结题
起止时间:
2017-05-01 至 2027-04-30

项目摘要

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中文摘要
翻译
摘要 申请补充资金以购买辉光放电装置,该装置几乎每天用于 由该R35资助的项目。负染色和低温电磁都需要电磁场栅极的辉光放电 清洁网格并使其更亲水的应用程序。这是示例中非常重要的一步 为冷冻-EM提供优质冰块的关键在于准备工作。现有的仪器是我实验室买的~ 13年前,它无法正常运行,无法以任何具有成本效益的方式进行修复。
英文摘要
Abstract Supplementary funds are requested to purchase a glow discharge apparatus to be used nearly daily for the projects funded by this R35. Glow discharge of EM grids is needed for both negative stain and cryo-EM applications to clean the grids and make them more hydrophilic. This is a very important step in sample preparation, and is crucial to getting good ice for cryo-EM. The existing apparatus was purchased in my lab ~ 13 years ago, is failing to function properly, and cannot be repaired in any cost-effective way.
期刊论文(44)
专著(0)
科研奖励(0)
会议论文
Helical reconstruction, again.
再次螺旋重建。
DOI: 10.1016/j.sbi.2024.102788
发表时间: 2024
期刊: Current opinion in structural biology
影响因子: 6.8
作者: [Egelman,EdwardH]
通讯作者: Egelman,EdwardH
DOI: 10.7554/elife.81551
发表时间: 2022-09-05
期刊: ELIFE
影响因子: 7.7
作者: [Wang, Fengbin, Chan, Chi Ho, Suciu, Victor, Mustafa, Khawla, Ammend, Madeline, Si, Dong, Hochbaum, Allon, I, Egelman, Edward H., Bond, Daniel R.]
通讯作者: Bond, Daniel R.
DOI: 10.1073/pnas.2207037119
发表时间: 2022-06-28
期刊: Proceedings of the National Academy of Sciences of the United States of America
影响因子: 11.1
作者: []
通讯作者:
DOI: 10.1038/s41564-022-01159-z
发表时间: 2022-08
期刊: Nature microbiology
影响因子: 28.3
作者: []
通讯作者:
共 24 条
    Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution
    • 批准号:
      10406567
    • 项目类别:
    • 资助金额:
      $84.56万
    • 财政年份:
      2017
    • 负责人:
      EDWARD H. EGELMAN
    • 依托单位:
    Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution
    • 批准号:
      10619013
    • 项目类别:
    • 资助金额:
      $75.25万
    • 财政年份:
      2017
    • 负责人:
      EDWARD H. EGELMAN
    • 依托单位:
    Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution
    • 批准号:
      9925804
    • 项目类别:
    • 资助金额:
      $67.54万
    • 财政年份:
      2017
    • 负责人:
      EDWARD H. EGELMAN
    • 依托单位:
    Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution
    • 批准号:
      10727070
    • 项目类别:
    • 资助金额:
      $7.4万
    • 财政年份:
      2017
    • 负责人:
      EDWARD H. EGELMAN
    • 依托单位:
    海外基金