Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜

• 批准号: 10793162
• 负责人: EDWARD H. EGELMAN
• 金额: $ 2.3万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10793162
• 关键词: Acids; Actins; Bacteria; Biotechnology; Cryoelectron Microscopy; Filament; Food Poisoning; Funding; Health; Human; Ice; Meningitis; Muscle; Negative Staining; Nucleoproteins; Organism; Polymers; Preparation; Proteins; Resolution; Sampling; Structure; Virus; cost effective; hydrophilicity; repaired; tool

Abstract Supplementary funds are requested to purchase a glow discharge apparatus to be used nearly daily for the projects funded by this R35. Glow discharge of EM grids is needed for both negative stain and cryo-EM applications to clean the grids and make them more hydrophilic. This is a very important step in sample preparation, and is crucial to getting good ice for cryo-EM. The existing apparatus was purchased in my lab ~ 13 years ago, is failing to function properly, and cannot be repaired in any cost-effective way.

摘要

项目成果

Helical reconstruction, again.

再次螺旋重建。

• DOI: 10.1016/j.sbi.2024.102788
• 发表时间: 2024
• 期刊: Current opinion in structural biology
• 影响因子: 6.8
• 作者: Egelman,EdwardH

Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms.

• DOI: 10.1073/pnas.2207037119
• 发表时间: 2022-06-28
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1

Structure of Geobacter OmcZ filaments suggests extracellular cytochrome polymers evolved independently multiple times.

• DOI: 10.7554/elife.81551
• 发表时间: 2022-09-05
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Wang, Fengbin;Chan, Chi Ho;Suciu, Victor;Mustafa, Khawla;Ammend, Madeline;Si, Dong;Hochbaum, Allon, I;Egelman, Edward H.;Bond, Daniel R.
• 通讯作者: Bond, Daniel R.

Neck and capsid architecture of the robust Agrobacterium phage Milano.

• DOI: 10.1038/s42003-023-05292-1
• 发表时间: 2023-09-08
• 期刊: COMMUNICATIONS BIOLOGY
• 影响因子: 5.9
• 作者: Sonani, Ravi R.;Esteves, Nathaniel C.;Horton, Abigail A.;Kelly, Rebecca J.;Sebastian, Amanda L.;Wang, Fengbin;Kreutzberger, Mark A. B.;Leiman, Petr G.;Scharf, Birgit E.;Egelman, Edward H.
• 通讯作者: Egelman, Edward H.

Cryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing.

• DOI: 10.1038/s41564-022-01159-z
• 发表时间: 2022-08
• 期刊: Nature microbiology
• 影响因子: 28.3