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300 keV Liquid Helium Robotic Microscope

300 keV 液氦机器人显微镜

基本信息

批准号：
7498169
负责人：
EDWARD H. EGELMAN
金额：
$ 200万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2011-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Funds are requested for purchase of a state-of-the-art 300 keV liquid helium Transmission Electron Cryo-Microscope with a Field Emission Gun. To leverage the funds provided by this NIH program, approximately 40% of the funds for this microscope will be derived from institutional sources at the University of Virginia. Two of the PIs use electron cryo-microscopy (cryo-EM) as their primary tool and will be the main users of this microscope. In addition, the acquisition of this instrument will be crucial for recruiting a third electron microscopist to the University of Virginia. There exists a significant community of NIH-funded investigators currently at the University of Virginia who will benefit greatly from the presence of this microscope. The need for this instrument arises from recent progress on a number of projects which show that existing microscopes at the University of Virginia limit continued progress. These projects include helical filaments, icosahedral viruses and two-dimensional crystals. Recent breakthroughs have been made in several areas, such as reconstructions of bacterial pili filamentous phage and HIV-1 capsids, and several indications suggest that much greater progress may be possible with a state-of-the-art microscope. The proposed microscope has an envelope function that decays much more slowly than the microscopes currently available, and this will allow us to obtain higher resolution structures. PUBLIC HEALTH RELEVANCE: We will use a higher resolution electron microscope to advance projects currently funded by the NIH. The higher resolution structures that we will obtain from this microscope will have an impact on matters of public health ranging from bacterial pathogenesis to heart disease to cancer to AIDS.

描述（由申请人提供）：资金要求购买一个国家的最先进的300千电子伏液氦透射电子低温显微镜与场发射枪。为了利用NIH项目提供的资金，该显微镜大约40%的资金将来自弗吉尼亚大学的机构来源。其中两名PI使用电子冷冻显微镜（cryo-EM）作为其主要工具，并将成为该显微镜的主要用户。此外，该仪器的收购将是至关重要的招聘第三电子显微镜到弗吉尼亚大学。目前在弗吉尼亚大学有一个由NIH资助的研究人员的重要社区，他们将从这种显微镜的存在中受益匪浅。对这种仪器的需要来自于最近一些项目的进展，这些项目表明弗吉尼亚大学现有的显微镜限制了持续的进展。这些项目包括螺旋细丝、二十面体病毒和二维晶体。最近在几个领域取得了突破，例如细菌皮利丝状噬菌体和HIV-1衣壳的重建，并且几个迹象表明，使用最先进的显微镜可能会取得更大的进展。拟议中的显微镜具有包络函数，其衰减速度比目前可用的显微镜慢得多，这将使我们能够获得更高分辨率的结构。公共卫生相关性：我们将使用更高分辨率的电子显微镜来推进目前由NIH资助的项目。我们将从这种显微镜中获得的更高分辨率的结构将对从细菌发病机制到心脏病到癌症到艾滋病的公共卫生问题产生影响。