Project 1: Identification and Validation of Panel of Early Cell Surface Gene Targets
Project 1: Identification and Validation of Panel of Early Cell Surface Gene Targets
批准号:
10155438
负责人:
DAVID George BEER
金额:
$15.26万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-09-21 至 2024-04-30
关键词:
AreaBarrett EsophagusBindingCandidate Disease GeneCell LineCell Surface ProteinsCell surfaceCellsCommunitiesCopy Number PolymorphismDNA DamageDNA copy numberDatabasesERBB2 geneEpidermal Growth Factor ReceptorEsophageal AdenocarcinomaEsophagusFrequenciesGamma-H2AXGene AmplificationGene ChipsGene ExpressionGene FrequencyGene MutationGene TargetingGenesGenomeGenomicsHumanImageImmunohistochemistryMessenger RNAMutationNeoadjuvant TherapyPeptidesPilot ProjectsRadiation therapyResearchResearch Project GrantsReverse Transcriptase Polymerase Chain ReactionSequence AnalysisSeriesSpecimenTelomeraseTissue MicroarrayTissuesValidationWestern Blottingbasebiobankcohortdimerexome sequencingexpression vectorfallsgene expression databasegene productgenomic toolsimaging agentimmunocytochemistrymonomernoveloverexpressiontranscriptome sequencingtumor heterogeneity
中文摘要
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英文摘要
Abstract – This research project falls under genomics of tumor heterogeneity and their impact on the
progression toward EAC as one of the possible areas defined in RFA-CA-16-006. We have previously
identified EGFR and ErbB2 using gene expression profiles as promising cell surface targets based on high-
frequency gene amplification for imaging of EAC, and have developed peptides that are specific for these
targets. We now aim to identify early targets that arise in progression of BE to EAC. We expect to find greater
variability and lower levels of expression for early compared with late targets, thus have prepared a more
rigorous approach. We will use Affymetrix arrays and RNAseq analysis to identify early gene targets that are
overexpressed on the cell surface and can be developed for imaging. We will use these sophisticated genomic
tools to provide a comprehensive analysis of gene alterations, mutations, and DNA copy number variations in
human esophagus specimens to identify a panel of promising candidates. We will rigorously validate
candidate targets using quantitative RT-PCR and immunohistochemistry on tissue microarrays prepared from
an independent cohort of specimens. This approach requires a large number of HGD that are uniquely
available in our large tissue biorepository of n = 780 human esophageal specimens, including n = 560 EAC,
collected de novo (no neo-adjuvant chemo or radiation therapy). For each early target validated, we will
transfect telomerase-immortalized BE cells to overexpress the target on the cell surface for use in developing
the monomer and dimer peptides in Project 2.
Successful completion of these aims will result in identification and validation of a panel of early gene
targets that arise in progression of BE to EAC that will be used in Project 2 to develop novel peptide imaging
agents.
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会议论文
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批准号:10219176
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项目类别:
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资助金额:$42.28万
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财政年份:2017
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负责人:DAVID George BEER
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依托单位:
Biomedical Computing and Informatics Strategies for Precision Medicine
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批准号:9366045
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项目类别:
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资助金额:$35.92万
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财政年份:2017
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负责人:DAVID George BEER
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依托单位:
RNF128 Regulation of TP53 in Barrett's Progression
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批准号:9976469
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项目类别:
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资助金额:$42.28万
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财政年份:2017
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负责人:DAVID George BEER
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依托单位:
Multi-Spectral Targeted Imaging for Early Detection of Cancer in Barrett's Esopha
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批准号:8724430
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项目类别:
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资助金额:$130.55万
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财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Early Targets in Progression of Barrett's Esophagus to Esophageal Adenocarcinoma
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批准号:9277831
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项目类别:
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资助金额:$108.42万
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财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Identification and Characterization of Gene Fusions in Lung Adenocarcinoma
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批准号:8018735
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项目类别:
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资助金额:$28.41万
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财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Identification and Characterization of Gene Fusions in Lung Adenocarcinoma
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批准号:8249361
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项目类别:
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资助金额:$28.62万
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财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Multi-Spectral Targeted Imaging for Early Detection of Cancer in Barrett's Esopha
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批准号:8919278
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项目类别:
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资助金额:$129.04万
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财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Multi-Spectral Targeted Imaging for Early Detection of Cancer in Barrett's Esopha
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批准号:8209767
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项目类别:
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资助金额:$114.45万
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财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Identification of Cell Surface Targets Based on Gene Amplification/Overexpression
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批准号:8244086
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项目类别:
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资助金额:$20.37万
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财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Identification and Characterization of Gene Fusions in Lung Adenocarcinoma
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批准号:8445146
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项目类别:
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资助金额:$26.94万
-
财政年份:2011
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负责人:DAVID George BEER
-
依托单位:
Multi-Spectral Targeted Imaging for Early Detection of Cancer in Barrett's Esopha
-
批准号:8539364
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项目类别:
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资助金额:$121.59万
-
财政年份:2011
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负责人:DAVID George BEER
-
依托单位:
Multi-Spectral Targeted Imaging for Early Detection of Cancer in Barrett's Esopha
-
批准号:8336829
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项目类别:
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资助金额:$131.58万
-
财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Identification of Cell Surface Targets Based on Gene Amplification/Overexpression
-
批准号:8555330
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项目类别:
-
资助金额:$41.79万
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财政年份:2011
-
负责人:DAVID George BEER
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依托单位:
Identification and Characterization of Gene Fusions in Lung Adenocarcinoma
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批准号:8618865
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项目类别:
-
资助金额:$27.84万
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财政年份:2011
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负责人:DAVID George BEER
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依托单位:
Task Specific Project 4
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批准号:7728720
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项目类别:
-
资助金额:$5.35万
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财政年份:2008
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负责人:DAVID George BEER
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依托单位:
LUNG TUMOR PROJECT
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批准号:6300695
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项目类别:
-
资助金额:$10.37万
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财政年份:2000
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负责人:DAVID George BEER
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依托单位:
LUNG TUMOR PROJECT
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批准号:6230239
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项目类别:
-
资助金额:$10.37万
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财政年份:1999
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负责人:DAVID George BEER
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依托单位:
MOLECULAR STUDIES OF ESOPHAGEAL ADENOCARCINOMA
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批准号:6748611
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项目类别:
-
资助金额:$30.55万
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财政年份:1997
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负责人:DAVID George BEER
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依托单位:
MOLECULAR STUDIES OF ESOPHAGEAL ADENOCARCINOMA
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批准号:6633198
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项目类别:
-
资助金额:$30.55万
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财政年份:1997
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负责人:DAVID George BEER
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依托单位:
海外基金