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Identification of Cell Surface Targets Based on Gene Amplification/Overexpression

基于基因扩增/过表达的细胞表面靶点鉴定

基本信息

批准号：
8555330
负责人：
DAVID George BEER
金额：
$ 41.79万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-21 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8555330
关键词：
Address Adenocarcinoma Area Binding Biopsy Specimen Candidate Disease Gene Carcinoma in Situ Cell Line Cell Surface Proteins Cell surface Cells Clinical Colorectal Cancer Databases Detection Development Dysplasia ERBB2 gene Endoscopes Epidermal Growth Factor Receptor Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Esophageal Esophageal Adenocarcinoma Esophageal Tissue Esophageal mucous membrane Excision Frequencies Gene Amplification Gene Expression Genes Goals Image Immunohistochemistry In Vitro Incidence Intervention Label MMP11 gene MMP3 gene Malignant Neoplasms Membrane Proteins Messenger RNA Metaplasia Methods Michigan Molecular Biology Neoplasms Operative Surgical Procedures Patients Peptides Premalignant Principal Investigator Proteins Research Reverse Transcriptase Polymerase Chain Reaction Roche brand of trastuzumab Sampling Scanning Screening for cancer Sequence Analysis Specimen Surface Surveillance Methods Telomerase Therapeutic Tissues Universities Validation Western Blotting base expression vector imaging modality immunocytochemistry improved innovation meetings molecular imaging neoplastic novel optical imaging overexpression

项目摘要

A panel of 15 candidate targets will be identifed based on both high-frequency amplified chromosomal regions and mRNA overexpression from a SNP array database of specimens of neoplastic Barrett's esophageal mucosa with support from Core B. Validafion of selected gene candidates will be performed on an independent set of 50 cases of Barrett's metaplasia, low-grade, high-grade dysplasia and adenocarcinoma using quantitative RT-PCR and at the protein level using fissue microarrays and immunohistochemistry with support from Core C. This panel will be reduced to the 5 best candidate amplified and/or overexpressed cell surface genes will be transfected into immortalized Barrett's metaplasia cells for in vitro selection of imaging peptides in Project 2 for imaging with a mulfi-spectral scanning flber endoscope developed in Project 3.

一组15个候选目标将根据两个高频放大来自肿瘤样本SNP阵列数据库的染色体区域和mRNA过表达 Barrett食管粘膜，支持核心B。将对选定的候选基因进行验证对50例Barrett化生、低度、高度异型增生和使用定量RT-PCR和使用裂隙微阵列在蛋白质水平检测腺癌，免疫组化支持核心C。这个小组将减少到5个最好的候选人放大和/或过表达的细胞表面基因将被转染到永生化的巴雷特化生细胞中，多光谱扫描纤维内窥镜成像的项目2中成像肽的体外选择在项目3中开发。