基于基因扩增/过表达的细胞表面靶点鉴定-猫眼课题宝

基于基因扩增/过表达的细胞表面靶点鉴定

批准号：

8555330

负责人：

DAVID George BEER

金额：

$41.79万

依托单位：

UNIVERSITY OF MICHIGAN AT ANN ARBOR

依托单位国家：

美国

项目类别：

财政年份：

2011

资助国家：

美国

项目状态：

已结题

起止时间：

2011-09-21 至 2016-08-31

来源：

https://reporter.nih.gov/project-details/8555330

关键词：

Address Adenocarcinoma Area Binding Biopsy Specimen Candidate Disease Gene Carcinoma in Situ Cell Line Cell Surface Proteins Cell surface Cells Clinical Colorectal Cancer Databases Detection Development Dysplasia ERBB2 gene Endoscopes Epidermal Growth Factor Receptor Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Esophageal Esophageal Adenocarcinoma Esophageal Tissue Esophageal mucous membrane Excision Frequencies Gene Amplification Gene Expression Genes Goals Image Immunohistochemistry In Vitro Incidence Intervention Label MMP11 gene MMP3 gene Malignant Neoplasms Membrane Proteins Messenger RNA Metaplasia Methods Michigan Molecular Biology Neoplasms Operative Surgical Procedures Patients Peptides Premalignant Principal Investigator Proteins Research Reverse Transcriptase Polymerase Chain Reaction Roche brand of trastuzumab Sampling Scanning Screening for cancer Sequence Analysis Specimen Surface Surveillance Methods Telomerase Therapeutic Tissues Universities Validation Western Blotting base expression vector imaging modality immunocytochemistry improved innovation meetings molecular imaging neoplastic novel optical imaging overexpression

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中文摘要

翻译

一组 15 个候选目标将根据高频放大和肿瘤样本 SNP 阵列数据库中的染色体区域和 mRNA 过表达 Barrett 食管粘膜在 Core B 的支持下进行。选定候选基因的验证将在对 50 例 Barrett 化生、低度、高度不典型增生和使用定量 RT-PCR 和使用组织微阵列在蛋白质水平上检测腺癌在 Core C 的支持下进行免疫组织化学。该小组将减少到 5 个最佳候选扩增和/或过表达的细胞表面基因将被转染至永生化巴雷特化生细胞中项目2中用于多光谱扫描光纤内窥镜成像的成像肽的体外选择在项目3中开发。

英文摘要

A panel of 15 candidate targets will be identifed based on both high-frequency amplified chromosomal regions and mRNA overexpression from a SNP array database of specimens of neoplastic Barrett's esophageal mucosa with support from Core B. Validafion of selected gene candidates will be performed on an independent set of 50 cases of Barrett's metaplasia, low-grade, high-grade dysplasia and adenocarcinoma using quantitative RT-PCR and at the protein level using fissue microarrays and immunohistochemistry with support from Core C. This panel will be reduced to the 5 best candidate amplified and/or overexpressed cell surface genes will be transfected into immortalized Barrett's metaplasia cells for in vitro selection of imaging peptides in Project 2 for imaging with a mulfi-spectral scanning flber endoscope developed in Project 3.

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