MHC Genetic Typing Core

MHC 基因分型核心

• 批准号: 10158635
• 负责人: Eric J. Vallender
• 金额: $ 7.32万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-09 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10158635
• 关键词: AIDS/HIV problem; Acceleration; Acquired Immunodeficiency Syndrome; Alleles; Animal Husbandry; Animals; Behavioral Genetics; Breeding; Budgets; Collaborations; Communities; DNA Library; Data; Databases; Development; Disease Progression; Ensure; Funding; Future; Genetic; Genetic Services; Genetic Variation; Genome; Genomics; Genotype; Geographic Locations; Geography; Goals; Grant; Haplotypes; Health; Immune response; Inbreeding; Infection; Informatics; Institution; MHC Class I Genes; Macaca; Macaca mulatta; Maintenance; Mind; Monitor; Other Genetics; Output; Population; Predisposition; Preventive Medicine; Primates; Process; Protocols documentation; Research; Research Activity; Research Personnel; Role; SIV; Scientist; Services; Source; Structure; System; Targeted Research; Testing; Time; Translational Research; Wisconsin; animal colony; animal data; base; data sharing; deep sequencing; design; evidence base; exhaustion; exome sequencing; experience; genetic information; genetic pedigree; genetic resource; genetic testing; genome sequencing; genomic data; genomic variation; improved; male; nonhuman primate; programs; reproductive; research study; simian human immunodeficiency virus; testing services; tool; whole genome; working group

Project Summary/Abstract - MHC Genetic Typing Core The SPF rhesus macaque breeding colony for AIDS research at the Tulane National Primate Research Center (TNPRC) is part of an extensive evidence-based, integrative, program that incorporates best practices in health surveillance, husbandry, preventive medicine, and behavioral and genetic management. The goals of the genetic management program are to 1) maintain genetic diversity and minimize inbreeding to support sustainable populations and 2) characterize class I MHC alleles in support of HIV/AIDS research activities. To this end, the TNPRC Genome Bank collects and stores materials from more than 9000 unique animals, with additional animals collected as they are produced. The colony is characterized for ancestral geographic-origin and parentage confirmed using a pan-NPRC SNP panel. MHC typing for all animals includes testing for 10 class I alleles, which serves the needs of most core and affiliate scientists, and MHC deep sequencing in collaboration with the Wisconsin National Primate Research Center for targeted research programs. Over the previous grant funding period whole genome sequencing (WGS) has been added for 164 key breeders, focusing on those males with the greatest reproductive output in the breeding colony, and whole exome sequencing (WES) from 72 additional animals. Funding for WGS on an additional 200 animals during the coming budget period is also anticipated. These efforts are supported by core TNPRC scientists in collaboration with researchers from other NPRCs and research institutions. These efforts are made in concert with the broader NPRC community through consortia activities, notably, the Nonhuman Primate Genetics and Genomics Working Group. Presently, the efforts of the genetic management program and MHC Genetic Typing Core are to continue to provide high- quality MHC characterization of the SPF breeding colony in support of HIV/AIDS research, to integrate MHC typing with other genetic and genomic data currently being characterized at the TNPRC, and to develop additional support structures for sharing this data with the research community. These efforts ensure the SPF rhesus macaque breeding colony is healthy, sustainable, and of maximal translational research value to the broader HIV/AIDS research community.

项目摘要/摘要-MHC基因分型核心 杜兰国家灵长类研究中心用于艾滋病研究的SPF猕猴繁殖群体 (TNPRC)是一个广泛的以证据为基础的综合计划的一部分，该计划纳入了卫生领域的最佳实践 监测、畜牧业、预防医学、行为和遗传管理。基因工程的目标 管理计划是1)保持遗传多样性，最大限度地减少近亲繁殖，以支持可持续发展 2)确定支持艾滋病毒/艾滋病研究活动的I类MHC等位基因。为此， TNPRC基因组库收集并存储了9000多种独特动物的材料，还有其他 动物在生产过程中被收集。殖民地的特点是祖先的地理起源和 使用泛NPRC SNP小组确认了亲子关系。所有动物的MHC分型包括对10个I类动物的测试 满足大多数核心和附属科学家需求的等位基因，以及合作中的MHC深度测序 与威斯康星州国家灵长类动物研究中心进行有针对性的研究项目。比上一次拨款更高 资助期增加了164个关键繁育品种的全基因组测序(WGS)，重点放在那些雄性 在繁育群体中繁殖产量最大，整个外显子组测序(WES)从72 其他动物。在下一个预算期间，为另外200只动物提供的WGS资金也是 已经预料到了。这些努力得到了TNPRC核心科学家的支持，并与来自其他国家的研究人员合作 全国人民代表大会和研究机构。这些努力是与更广泛的NPRC社区通过 财团活动，特别是非人灵长类遗传学和基因组学工作组。目前， 基因管理计划和MHC基因分型核心的努力是继续提供高 支持HIV/AIDS研究的SPF繁殖群体的高质量MHC特征，以整合MHC 与TNPRC目前正在表征的其他遗传和基因组数据进行分型，并开发 用于与研究社区共享这些数据的其他支持结构。这些努力确保了SPF 恒河猴繁育群体是健康的、可持续的，对 更广泛的艾滋病毒/艾滋病研究界。