MHC Genetic Typing Core

项目概要/摘要- MHC基因分型核心 杜兰国家灵长类动物研究中心用于艾滋病研究的SPF恒河猴繁殖群 （TNPRC）是一个广泛的循证综合计划的一部分，该计划结合了健康领域的最佳实践 监测、饲养、预防医学以及行为和遗传管理。遗传学的目标 管理计划是1）保持遗传多样性，尽量减少近亲繁殖，以支持可持续发展 人群和2）表征I类MHC等位基因，以支持HIV/AIDS研究活动。为此中央 TNPRC基因组库收集和储存了9000多种独特动物的材料， 动物在生产时被收集。殖民地的特点是祖先的地理起源， 使用泛NPRC SNP组确认亲子关系。所有动物的MHC分型包括10种I类抗原检测 等位基因，满足大多数核心和附属科学家的需求，并与MHC深度测序合作 与威斯康星州国家灵长类动物研究中心进行有针对性的研究项目。超过了之前的补助金 在资助期内，为164只主要育种者增加了全基因组测序（WGS），重点是那些雄性 在繁殖群体中具有最大的繁殖输出，并且从72 更多动物在下一个预算期内，还为WGS额外饲养200只动物提供资金 预期。这些努力得到了TNPRC核心科学家与其他研究人员合作的支持 NPRC和研究机构。这些努力是与更广泛的NPRC社区协调一致的， 非人类灵长类动物遗传学和基因组学工作组（Nonhuman Primate Genetics and Genomics Working Group）如今 基因管理计划和MHC基因分型核心的努力是继续提供高， 高质量的SPF繁殖群体的MHC特性，以支持HIV/AIDS研究，整合MHC 与目前在TNPRC表征的其他遗传和基因组数据进行分型，并开发 与研究界分享这些数据的额外支持结构。这些努力确保了SPF 恒河猴繁殖群是健康的，可持续的，对人类具有最大的转化研究价值。 更广泛的艾滋病毒/艾滋病研究界。

Project Summary/Abstract - MHC Genetic Typing Core The SPF rhesus macaque breeding colony for AIDS research at the Tulane National Primate Research Center (TNPRC) is part of an extensive evidence-based, integrative, program that incorporates best practices in health surveillance, husbandry, preventive medicine, and behavioral and genetic management. The goals of the genetic management program are to 1) maintain genetic diversity and minimize inbreeding to support sustainable populations and 2) characterize class I MHC alleles in support of HIV/AIDS research activities. To this end, the TNPRC Genome Bank collects and stores materials from more than 9000 unique animals, with additional animals collected as they are produced. The colony is characterized for ancestral geographic-origin and parentage confirmed using a pan-NPRC SNP panel. MHC typing for all animals includes testing for 10 class I alleles, which serves the needs of most core and affiliate scientists, and MHC deep sequencing in collaboration with the Wisconsin National Primate Research Center for targeted research programs. Over the previous grant funding period whole genome sequencing (WGS) has been added for 164 key breeders, focusing on those males with the greatest reproductive output in the breeding colony, and whole exome sequencing (WES) from 72 additional animals. Funding for WGS on an additional 200 animals during the coming budget period is also anticipated. These efforts are supported by core TNPRC scientists in collaboration with researchers from other NPRCs and research institutions. These efforts are made in concert with the broader NPRC community through consortia activities, notably, the Nonhuman Primate Genetics and Genomics Working Group. Presently, the efforts of the genetic management program and MHC Genetic Typing Core are to continue to provide high- quality MHC characterization of the SPF breeding colony in support of HIV/AIDS research, to integrate MHC typing with other genetic and genomic data currently being characterized at the TNPRC, and to develop additional support structures for sharing this data with the research community. These efforts ensure the SPF rhesus macaque breeding colony is healthy, sustainable, and of maximal translational research value to the broader HIV/AIDS research community.