FDA LFFM: Discipline-Special Projects Analytical Track-Method Development and Method Validation at CT AES

FDA LFFM：CT AES 的学科特殊项目分析跟踪方法开发和方法验证

• 批准号: 10170743
• 负责人: JASON C WHITE
• 金额: $ 3.5万
• 依托单位: CONNECTICUT AGRICULTURAL EXPERIMENT STA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10170743
• 关键词: FDA; LFFM; Discipline; Special; Projects

PROJECT SUMMARY- METHOD DEVELOPMENT AND VALIDATION SPECIAL PROJECT FDA LFFM: Discipline-Special Projects; Method Development and Validation at CT AES Project Summary- This proposal is being submitted in response to RFA-PAR-20-105 Laboratory Flexible Funding Model (LFFM)(U19). The Connecticut Agricultural Experiment Station (CT AES) has a 145-year history of food safety research, including 15 years as a member of the FDA Food Emergency Response Network (FERN) Chemistry Cooperative Agreement Program (cCAP), and 8 years of funding under 4 different FDA ISO food safety CAPs. For this Method Development and Validation Project, the goal is conduct preliminary, short-term or exploratory investigations that focus on the feasibility of a new method and/or technology. We propose 9 projects that include activities such as proof of concept for new analytical approaches, method development for representative food matrices, single laboratory validations of methods, matrix or platform extensions or multi-laboratory validation research studies. Some of these projects could become multi-year projects, dependent on results and FDA approval. We also recognize that project opportunities may change annually, as requested by FDA. These projects are: 1) Single laboratory validation of LC-MS/MS methods for pesticides in new matrices. 2) Multi-laboratory validation of Elemental Analysis Manual method EAM 4.12: analysis of bottled water by ICP- MS. 3) Protein analysis by LC-HRMS and LC-MS/MS. 4) Portable Hand-Held X-Ray Fluorescence Determination of Toxic Elements EAM 4.9. 5) Multi-laboratory validation of method for Arsenic speciation in seafood using LC-ICP-MS. 6) Detection of PFASs in animal feed using LC-HRMS/MS. 7) Multi-laboratory validation LC/MS/MS method for vet drug residues in raw milk, milk, and powdered milk. 8) Non-targeted analysis of unknown toxins in food using LC- HRMS/MS, Compound Discoverer, and Hydrophilic Interaction Chromatography (HILIC). 9) Single laboratory validation of mycotoxins in hemp and hemp-derived products. The CT AES has demonstrated the expertise, capability, and adaptability to fully meet the objectives of this RFA through our successful participation in a number of FDA CAPs. Receipt of this new award from FDA will significantly enhance the ability of the CT AES specifically, and CT in general, to meet the program goals laid out in the RFA, and as described by the President’s Food Safety Working Group, the Partnership for Food Protection and the Food Safety Modernization Act. 1

项目总结-方法开发和验证 特别项目 FDA LFFM：麻黄碱-特殊项目; CT AES的方法开发和验证 项目摘要-本提案是根据RFA-PAR-20-105实验室提交的 灵活供资模式（LFFM）（U19）。康涅狄格州农业实验站（Connecticut Agricultural Experiment Station） AES）拥有145年的食品安全研究历史，其中15年是FDA的成员 粮食紧急反应网络化学合作协议计划 （cCAP），以及在4个不同的FDA ISO食品安全CAP下的8年资助。对于这种方法 开发和验证项目，目标是进行初步，短期或探索性 重点关注新方法和/或技术的可行性的调查。我们建议9 项目包括新分析方法的概念验证、方法 代表性食品基质的开发，方法的单一实验室验证，基质或 平台扩展或多实验室验证研究。其中一些项目可以 成为多年项目，取决于结果和FDA的批准。我们也认识到 根据FDA的要求，项目机会每年可能会发生变化。这些项目是：1）单一 LC-MS/MS方法在新基质中农药的实验室验证。2)多实验室 元素分析手册方法EAM 4.12的验证：通过ICP分析瓶装水- 女士3)通过LC-HRMS和LC-MS/MS进行蛋白质分析。4）便携式手持式X射线 有毒元素的荧光测定EAM 4.9. 5)多实验室验证 水产品中砷形态的LC-ICP-MS分析方法6）动物饲料中PFASs的检测 7）兽医药物残留的多实验室验证LC/MS/MS方法 生牛奶、牛奶和奶粉。8)LC-1000非靶向分析食品中的未知毒素 HRMS/MS、化合物发现者和亲水作用色谱（HILIC）。九、 大麻和大麻衍生产品中霉菌毒素的单一实验室验证。 CT AES已经证明了其专业知识、能力和适应性，可以完全满足 我们通过成功参与多项FDA CAP来实现RFA的目标。收到 FDA的这一新奖项将显著提高CT AES的能力， CT一般，以满足RFA中规定的计划目标，并如总统的 食物安全工作小组、食物保护伙伴关系及食物安全 现代化法案。 1