Expansion of FERN Activities at the Connecticut Agricultural Experiment Station (CAES)

康涅狄格州农业实验中蕨类活动的扩展 车站(CAES) 化学恐怖主义或掺假对食品供应的污染是一个重大问题。 关注和粮食紧急反应网络(FERN)是在直接反应中建立的 面对这一威胁。弗恩的目标是预防、准备、反应和恢复，尊重 任何涉及威胁国家食品供应安全和完整性的事件。美国食品和药物管理局 弗恩寻求直接提高国家、地方和部落的能力和能力 政府的食品检测实验室建立综合食品安全体系。具体来说， 需要训练有素的人员，拥有必要的专业知识、用品和仪器设备 处理应对突发事件所需的冗余和实验室浪涌能力 食物供应污染的问题。中国科学院分析化学系已有120年的历史 食品安全研究历史，包括作为CCAP实验室的十年。我们在中国的演出 CCAP直接解决了FERN的目标，并在几个方面帮助加强了网络。我们 在每个蕨类化学项目领域建立和发展专业知识，在方法上出类拔萃 评估，对能力测试和监督样本作出准确和快速的反应； 提供了分析洞察力，改进了现有的FERN方法，与 其他州和联邦实验室，经常在国家和技术会议上介绍 计划工作，扩展了可以使用 现有的方法和开发的方法来确定额外的威胁剂和杀虫剂。 目前的提案分为四个不同的项目领域，所有这些领域都具有内在的灵活性 根据法医化学中心(FCC)和FERN国家项目办公室(NPO)的投入： (1)利用GC-MS分析对有毒、有毒物质进行筛选和鉴定 物质，以及食品中的未知化合物。在这个项目领域，我们将扩大我们的工作 通过在新部署的蕨类5977A上验证Fern-CHE.0006.00，验证了这种方法 平台，调查该仪器上的新反卷积报告软件(DRS)，协助 FDA在监测任务中，检查新的毒素和毒物，调查新的基质 作为粉末酒精和海鲜，并继续教授FDA GC-MS LB508课程。 (2)利用LC-MS分析筛选和鉴定有毒、有毒物质 物质，以及食品中的未知化合物。在这里，我们建议在新的 部署了Q-Exactive高分辨率(HR)LC-MS，以探索HR LC-MS(Q- 用于分析食品中的杀虫剂/霉菌毒素/化疗药物 检测新的毒素和毒物，研究新的色谱柱，协助FDA 监测任务，并使用HR LC-MS来研究蓖麻毒素和ABRIN的生物标志物。 (3)电感耦合等离子体质谱在重金属和有毒物质筛选鉴定中的应用 食物中的元素。我们建议完成EAM 4.7的方法验证，以调查 元素污染新的基质，以协助FDA的监测任务，以扩大 LC-ICPMS用于食品中砷的形态分析，并验证单粒子电感耦合等离子体质谱作为一种 食品中纳米颗粒的检测方法。 (4)使用酶联免疫吸附试验和其他基于抗体的分析进行筛查和 食品中未知毒素的鉴定。我们建议将酶联免疫吸附试验扩展到新的污染物 和关注的矩阵，以评估新的毒素试剂盒和平台，以协助FDA进行监测 并研究了不同条件下食物中蓖麻毒素和苦参碱的稳定性。 这个机会的明确目标是支持FDA对食品进行分析，以支持 一个全国性的综合食品安全体系。中国科学院分析化学系 始终如一地展示了完全满足并超越这些目标的专业知识、能力和适应能力 计划需求和此融资机会将扩展和优化此协作。

EXPANSION OF FERN ACTIVITIES AT THE CONNECTICUT AGRICULTURAL EXPERIMENT STATION (CAES) Contamination of the food supply from chemical terrorism or adulteration is an issue of great concern and the Food Emergency Response Network (FERN) was established in direct response to this threat. The FERN goals are Prevention, Preparedness, Response, and Recovery with respect to any incident involving a threat to the safety and integrity of the national food supply. The FDA FERN seeks to directly enhance the capacity and capability of State, Local, and Tribal government’s food testing laboratories to establish an Integrated Food Safety System. Specifically, there needs to be trained personnel with the expertise, supplies and instrumentation necessary to handle the redundancy and laboratory surge capacity that will be required to respond to incidences of food supply contamination. The CAES Department of Analytical Chemistry has a 120 year history of food safety research, including ten years as a cCAP laboratory. Our performance in cCAP has directly addressed FERN goals and helped strengthen the network in several ways. We have built on and developed expertise in each FERN Chemistry Project Area, excelled at method evaluation, provided accurate and quick response to proficiency testing and surveillance samples, provided analytical insight that has enhanced existing FERN methods, communicated well with other state and federal laboratories, presented frequently at national and technical meetings on program work, extended the number of threat agents and pesticides that can be analyzed using the existing methodology and developed methods to determine additional threat agents and pesticides. The current proposal is divided into four distinct Project Areas, all of which are inherently flexible depending on Forensic Chemistry Center (FCC) and FERN National Program Office (NPO) input: (1) The use of GC-MS analysis for the screening and identification of poisons, toxic substances, and unknown compounds in foods. In this Project Area, we will expand our work with this methodology by validating FERN-CHE.0006.00 on the newly deployed FERN 5977A platform, investigate the new deconvolution reporting software (DRS) on this instrument, assist FDA in surveillance assignments, examine new toxins and poisons, investigate new matrices such as powdered alcohol and seafood, and continue teaching the FDA GC-MS LB508 course. (2) The use of LC-MS analysis for the screening and identification of poisons, toxic substances, and unknown compounds in foods. Here, we propose to validate TO22 on the newly deployed Q-Exactive High Resolution (HR) LC-MS, to explore the use of HR LC-MS (Q- Exactive, Exactive) for the analysis of pesticides/mycotoxins/chemotherapeutics in food, to examine new toxins and poisons, to investigate new chromatographic columns, to assist FDA in surveillance assignments, and to use HR LC-MS to investigate biomarkers for ricin and abrin. (3) The use of ICP-MS for the screening and identification of heavy metals and toxic elements in foods. We propose to complete the method validation of EAM 4.7, to investigate elemental contamination of new matrices, to assist FDA in surveillance assignments, to expand the use of LC-ICP-MS for arsenic speciation in foods, and to validate single particle ICP-MS as a method for nanoparticle detection in food. (4) The use of ELISA and other antibody-based analyses for the screening and identification of unknown toxins in foods. We propose to extend ELISA to new contaminants and matrices of concern, to evaluate new kits and platforms for toxins, to assist FDA in surveillance assignments and to investigate ricin and abrin stability in foods under different conditions. The explicit goal of this opportunity is to enable the analysis of food for the FDA in support of a Nationally Integrated Food Safety System. The CAES Department of Analytical Chemistry has consistently demonstrated the expertise, capability, and adaptability to fully meet and exceed these program needs and this funding opportunity will expand and optimize this collaboration.