Expansion of FERN Activities at the Connecticut Agricultural Experiment Station (CAES)

康乃狄克农业试验中蕨类植物活动的扩大 车站（CAES） 化学恐怖主义或掺假对食品供应的污染是一个重大问题， 粮食紧急反应网络（FERN）的建立， 这一威胁。FERN的目标是预防、准备、响应和恢复， 任何涉及威胁国家食品供应安全和完整性的事件。FDA的 FERN旨在直接提高国家，地方和部落的能力和能力 政府的食物化验所，以建立一个综合食物安全系统。具体地说， 需要有经过培训的人员，他们具有必要的专业知识、用品和仪器， 处理应对突发事件所需的冗余和实验室激增能力 食品供应污染。CAES分析化学系拥有120年的 食品安全研究的历史，包括作为cCAP实验室的十年。我们的表现 cCAP直接解决了FERN的目标，并以多种方式帮助加强了网络。我们 我在每个FERN化学项目领域建立并发展了专业知识，擅长方法 评估，提供准确和快速的反应能力测试和监督样品， 提供了分析见解，增强了现有的FERN方法，与 其他州和联邦实验室，经常在国家和技术会议上提出， 计划工作，扩大了可以使用 现有的方法和开发的方法，以确定其他威胁剂和农药。 目前的建议分为四个不同的项目领域，所有这些领域都具有内在的灵活性 根据法医化学中心（FCC）和FERN国家计划办公室（NPO）的输入： (1)GC-MS分析在毒物筛选和鉴定中的应用 食物中的未知物质和未知化合物。在这个项目区，我们将扩大我们的工作， 通过在新部署的FERN 5977 A上验证FERN-CHE.0006.00， 平台，调查该仪器上的新去卷积报告软件（DRS），协助 FDA在监督任务中，检查新的毒素和毒药，调查新的基质， 作为粉状酒精和海鲜，并继续教授FDA GC-MS LB 508课程。 (2)LC-MS分析在毒物筛选和鉴定中的应用 食物中的未知物质和未知化合物。在这里，我们建议在新的 部署了Q-Exactive高分辨率（HR）LC-MS，以探索HR LC-MS（Q-Exactive）的使用。 Exactive，Exactive）用于分析食品中的农药/真菌毒素/化疗药物，以 检查新的毒素和毒物，研究新的色谱柱，协助FDA 监测分配，并使用HR LC-MS研究蓖麻毒素和相思豆毒素的生物标志物。 (3)ICP-MS用于重金属和有毒物质的筛选和鉴定 食物中的元素。我们建议完成EAM 4.7的方法验证，以调查 新基质的元素污染，以协助FDA进行监督任务， LC-ICP-MS用于食品中砷的形态分析，并验证单颗粒ICP-MS作为 食品中纳米颗粒的检测方法 (4)使用ELISA和其他基于抗体的分析进行筛选和 确定食物中的未知毒素。我们建议将ELISA扩展到新的污染物 和关注矩阵，评估新的毒素试剂盒和平台，协助FDA进行监测， 任务，并研究蓖麻毒素和相思豆毒素在不同条件下的食品稳定性。 这个机会的明确目标是使食品和药物管理局的食品分析，以支持 全国统一的食品安全体系。CAES分析化学系拥有 一贯表现出的专业知识，能力和适应能力，以充分满足和超越这些 计划需求和这一资金机会将扩大和优化这种合作。

EXPANSION OF FERN ACTIVITIES AT THE CONNECTICUT AGRICULTURAL EXPERIMENT STATION (CAES) Contamination of the food supply from chemical terrorism or adulteration is an issue of great concern and the Food Emergency Response Network (FERN) was established in direct response to this threat. The FERN goals are Prevention, Preparedness, Response, and Recovery with respect to any incident involving a threat to the safety and integrity of the national food supply. The FDA FERN seeks to directly enhance the capacity and capability of State, Local, and Tribal government’s food testing laboratories to establish an Integrated Food Safety System. Specifically, there needs to be trained personnel with the expertise, supplies and instrumentation necessary to handle the redundancy and laboratory surge capacity that will be required to respond to incidences of food supply contamination. The CAES Department of Analytical Chemistry has a 120 year history of food safety research, including ten years as a cCAP laboratory. Our performance in cCAP has directly addressed FERN goals and helped strengthen the network in several ways. We have built on and developed expertise in each FERN Chemistry Project Area, excelled at method evaluation, provided accurate and quick response to proficiency testing and surveillance samples, provided analytical insight that has enhanced existing FERN methods, communicated well with other state and federal laboratories, presented frequently at national and technical meetings on program work, extended the number of threat agents and pesticides that can be analyzed using the existing methodology and developed methods to determine additional threat agents and pesticides. The current proposal is divided into four distinct Project Areas, all of which are inherently flexible depending on Forensic Chemistry Center (FCC) and FERN National Program Office (NPO) input: (1) The use of GC-MS analysis for the screening and identification of poisons, toxic substances, and unknown compounds in foods. In this Project Area, we will expand our work with this methodology by validating FERN-CHE.0006.00 on the newly deployed FERN 5977A platform, investigate the new deconvolution reporting software (DRS) on this instrument, assist FDA in surveillance assignments, examine new toxins and poisons, investigate new matrices such as powdered alcohol and seafood, and continue teaching the FDA GC-MS LB508 course. (2) The use of LC-MS analysis for the screening and identification of poisons, toxic substances, and unknown compounds in foods. Here, we propose to validate TO22 on the newly deployed Q-Exactive High Resolution (HR) LC-MS, to explore the use of HR LC-MS (Q- Exactive, Exactive) for the analysis of pesticides/mycotoxins/chemotherapeutics in food, to examine new toxins and poisons, to investigate new chromatographic columns, to assist FDA in surveillance assignments, and to use HR LC-MS to investigate biomarkers for ricin and abrin. (3) The use of ICP-MS for the screening and identification of heavy metals and toxic elements in foods. We propose to complete the method validation of EAM 4.7, to investigate elemental contamination of new matrices, to assist FDA in surveillance assignments, to expand the use of LC-ICP-MS for arsenic speciation in foods, and to validate single particle ICP-MS as a method for nanoparticle detection in food. (4) The use of ELISA and other antibody-based analyses for the screening and identification of unknown toxins in foods. We propose to extend ELISA to new contaminants and matrices of concern, to evaluate new kits and platforms for toxins, to assist FDA in surveillance assignments and to investigate ricin and abrin stability in foods under different conditions. The explicit goal of this opportunity is to enable the analysis of food for the FDA in support of a Nationally Integrated Food Safety System. The CAES Department of Analytical Chemistry has consistently demonstrated the expertise, capability, and adaptability to fully meet and exceed these program needs and this funding opportunity will expand and optimize this collaboration.