Administrative Supplement: Mechanisms of Spliceosome Assembly and Regulation

行政补充：剪接体组装与调控机制

• 批准号: 10169637
• 负责人: Aaron Andrew Hoskins
• 金额: $ 0.58万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10169637
• 关键词: Administrative Supplement; Award; Biochemical; Biotechnology; Communication; Complex; Computer Models; Computers; Cryoelectron Microscopy; Development; Disease; Drug Industry; E-learning; Funding; Future; Goals; Group Meetings; Human; Laboratories; Location; Maps; Mentors; Missense Mutation; Modeling; Mutation; Parents; Positioning Attribute; Professional Competence; RNA Splicing; Regulation; Research; Research Personnel; Research Project Grants; Resources; Spliceosome Assembly Pathway; Spliceosomes; Structure; Techniques; Time; Training; Writing; career; career development; disease-causing mutation; experience; human disease; human model; mRNA Precursor; meetings; skills; structural biology; undergraduate research

PROJECT SUMMARY/ABSTRACT The overarching goals of the parent R35 award is to elucidate mechanisms of spliceosome assembly and regulation in biochemical depth using a variety of techniques. This administrative supplement will support undergraduate research, scientific development, and career development and contribute to these goals. The candidate (Lukas Voigts) will complete a full-time virtual learning and research experience during the Summer of 2020 in my laboratory. He will annotate human spliceosome cryo-EM structures with the locations of human disease-causing mutations. This will allow him to study the structure/function impact of these mutations. In addition, he will create Pymol session files and scripts to allow others to view his annotated structures. These files will be a valuable addition to the field for others studying pre-mRNA splicing and human disease. As part of this training, Lukas will gain new technical and operational skills in structural biology and writing computer scripts for Pymol. He will also be mentored in scientific research, communication, and career development with an aim towards meeting his own career goals of a position in the biotechnology or pharmaceutical industry upon graduation.

项目总结/摘要 父R35奖的首要目标是阐明剪接体组装的机制， 使用各种技术在生化深度上进行调节。这一行政补充将支持 本科研究，科学发展和职业发展，并有助于这些目标。的 候选人（卢卡斯Voigts）将在夏季完成全职的虚拟学习和研究经验 2020年在我的实验室里他将注释人类剪接体cryo-EM结构的位置， 致病突变这将使他能够研究这些突变的结构/功能影响。在 此外，他将创建Pymol会话文件和脚本，以允许其他人查看他的注释结构。这些 文件将是一个有价值的除了该领域的其他研究前mRNA剪接和人类疾病。的一部分 通过这次培训，Lukas将获得结构生物学和编写计算机脚本方面的新技术和操作技能 对于Pymol。他还将接受科学研究，沟通和职业发展方面的指导， 实现自己在生物技术或制药行业的职业目标， 毕业典礼