Administrative Supplement: Mechanisms of Spliceosome Assembly and Regulation

行政补充：剪接体组装与调控机制

• 批准号: 10169637
• 负责人: Aaron Andrew Hoskins
• 金额: $ 0.58万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10169637
• 关键词: Administrative Supplement; Award; Biochemical; Biotechnology; Communication; Complex; Computer Models; Computers; Cryoelectron Microscopy; Development; Disease; Drug Industry; E-learning; Funding; Future; Goals; Group Meetings; Human; Laboratories; Location; Maps; Mentors; Missense Mutation; Modeling; Mutation; Parents; Positioning Attribute; Professional Competence; RNA Splicing; Regulation; Research; Research Personnel; Research Project Grants; Resources; Spliceosome Assembly Pathway; Spliceosomes; Structure; Techniques; Time; Training; Writing; career; career development; disease-causing mutation; experience; human disease; human model; mRNA Precursor; meetings; skills; structural biology; undergraduate research

PROJECT SUMMARY/ABSTRACT The overarching goals of the parent R35 award is to elucidate mechanisms of spliceosome assembly and regulation in biochemical depth using a variety of techniques. This administrative supplement will support undergraduate research, scientific development, and career development and contribute to these goals. The candidate (Lukas Voigts) will complete a full-time virtual learning and research experience during the Summer of 2020 in my laboratory. He will annotate human spliceosome cryo-EM structures with the locations of human disease-causing mutations. This will allow him to study the structure/function impact of these mutations. In addition, he will create Pymol session files and scripts to allow others to view his annotated structures. These files will be a valuable addition to the field for others studying pre-mRNA splicing and human disease. As part of this training, Lukas will gain new technical and operational skills in structural biology and writing computer scripts for Pymol. He will also be mentored in scientific research, communication, and career development with an aim towards meeting his own career goals of a position in the biotechnology or pharmaceutical industry upon graduation.

项目摘要/摘要 父母R35奖的总体目标是阐明剪接体大会的机制和 使用多种技术在生化深度进行调节。这种行政补充将支持 本科研究，科学发展和职业发展，并为这些目标做出了贡献。这 候选人（Lukas Voigts）将在夏季完成全日制的虚拟学习和研究经验 2020年在我的实验室。他将注释人类的剪接体冷冻EM结构与人类的位置 引起疾病的突变。这将使他能够研究这些突变的结构/功能影响。在 此外，他将创建Pymol会话文件和脚本，以允许其他人查看他的带注释的结构。这些 对于其他研究MRNA剪接和人类疾病的人来说，文件将是该领域的宝贵补充。作为 在这项培训中，卢卡斯将获得结构生物学的新技术和运营技巧，并编写计算机脚本 用于皮摩尔。他还将在科学研究，沟通和职业发展中受到指导 实现他自己的职业目标，即在生物技术或制药行业中的职位目标 毕业。