Administrative Supplement: Mechanisms of Spliceosome Assembly and Regulation

行政补充：剪接体组装与调控机制

• 批准号: 10169637
• 负责人: Aaron Andrew Hoskins
• 金额: $ 0.58万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10169637
• 关键词: Administrative Supplement; Award; Biochemical; Biotechnology; Communication; Complex; Computer Models; Computers; Cryoelectron Microscopy; Development; Disease; Drug Industry; E-learning; Funding; Future; Goals; Group Meetings; Human; Laboratories; Location; Maps; Mentors; Missense Mutation; Modeling; Mutation; Parents; Positioning Attribute; Professional Competence; RNA Splicing; Regulation; Research; Research Personnel; Research Project Grants; Resources; Spliceosome Assembly Pathway; Spliceosomes; Structure; Techniques; Time; Training; Writing; career; career development; disease-causing mutation; experience; human disease; human model; mRNA Precursor; meetings; skills; structural biology; undergraduate research

PROJECT SUMMARY/ABSTRACT The overarching goals of the parent R35 award is to elucidate mechanisms of spliceosome assembly and regulation in biochemical depth using a variety of techniques. This administrative supplement will support undergraduate research, scientific development, and career development and contribute to these goals. The candidate (Lukas Voigts) will complete a full-time virtual learning and research experience during the Summer of 2020 in my laboratory. He will annotate human spliceosome cryo-EM structures with the locations of human disease-causing mutations. This will allow him to study the structure/function impact of these mutations. In addition, he will create Pymol session files and scripts to allow others to view his annotated structures. These files will be a valuable addition to the field for others studying pre-mRNA splicing and human disease. As part of this training, Lukas will gain new technical and operational skills in structural biology and writing computer scripts for Pymol. He will also be mentored in scientific research, communication, and career development with an aim towards meeting his own career goals of a position in the biotechnology or pharmaceutical industry upon graduation.

项目摘要/摘要 父R35奖的首要目标是阐明剪接体组装和 使用各种技术对生化深度进行调控。这份行政副刊将支持 本科生研究、科学发展和职业发展，并为这些目标做出贡献。这个 考生(Lukas Voigts)将在暑期完成全日制虚拟学习和研究体验 在我的实验室里。他将用人类的位置来注释人类的剪接体冷冻-EM结构 致病突变。这将使他能够研究这些突变对结构/功能的影响。在……里面 此外，他还将创建PYMOL会话文件和脚本，以允许其他人查看他的注释结构。这些 对于其他研究前-信使核糖核酸剪接和人类疾病的人来说，这些文件将是该领域的宝贵补充。作为以下内容的一部分 通过这次培训，卢卡斯将在结构生物学和编写计算机脚本方面获得新的技术和操作技能 为了皮莫尔。他还将在科学研究、交流和职业发展方面接受指导，目的是 为了实现他自己的职业目标，在生物技术或制药行业担任一个职位 毕业典礼。