权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mechanoregulation of Cell Functions during Embryogenesis

胚胎发生过程中细胞功能的机械调节

基本信息

批准号：
10170395
负责人：
SUSAN M PARKHURST
金额：
$ 16.37万
依托单位：
FRED HUTCHINSON CANCER RESEARCH CENTER
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-20 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10170395
关键词：
Actins Actomyosin Affect Annexins Behavior Biochemical Biological Biomechanics Biophysics Cell membrane Cell physiology Cell-Cell Adhesion Cells Characteristics Complement Complex Cues Cytokinesis Cytoskeletal Proteins Cytoskeleton Development Developmental Cell Biology Disease Drosophila genus Due Process Embryo Embryonic Development Event Failure Foundations Future Generations Genetic Goals Image Individual Learning Mammalian Cell Mechanics Membrane Microfilaments Mitochondria Modeling Molecular Morphogenesis Morphology Muscular dystrophy cardiomyopathy Nature Neoplasm Metastasis Pathway interactions Process Property Proteins Reagent Regulation Role Route Signal Transduction Stress System Techniques Tissues Work behavior influence cell behavior cell cortex clinically relevant developmental disease experimental study genetic approach imaging approach in vivo imaging insight interest mechanical properties mechanotransduction prevent response scaffold tumor progression wound wound healing

项目摘要

PROJECT SUMMARY Sculpting complex morphological body plans requires the precise orchestration of biophysical and biochemical cues to control the wide range of behaviors demanded of individual cells, as well as groups of cells. Biomechanical forces at the cell level affect the functions of the cell cortex: the plasma membrane and its underlying cortical cytoskeleton. These mechanical cues must be sensed by the cell, then properly acted upon, with the failure to do so leading to abnormal development. Thus, cell mechanoregulation is of fundamental developmental and cell biology interest and significant clinical relevance. The general aim of this proposal is to delineate the contribution of mechanoregulatory signals to cell functions required for normal development, and the consequences of their mis-regulation leading to aberrant cell functions and/or developmental disorders. Drosophila provides an excellent, genetically amenable, model in which to investigate these fundamental processes due to its accessibility to dynamic in vivo imaging and the wealth of state-of-the-art developmental/cell/molecular techniques and reagents available. Our long-term goal is to understand how mechanical cues are sensed, then acted upon, by cells to guide their functions during embryogenesis. To this end, we propose to use the forces generated by cellular wounding as an inducible system in which to study the mechanical properties of the cell cortex, including membrane tension, cortical cytoskeleton dynamics and the integration of these properties. The specific aims of this proposal are: 1) to determine the nature of the scaffold at the embryo cortex involved in tension regulation, and 2) to elucidate the mechanisms regulating actomyosin organization necessary for generating contractile forces within cells. The information gathered in these studies will provide new insight into the mechanical characteristics of the cell, as well as provide a better understanding of how the cell interprets the intrinsic and extrinsic forces acting upon it to orchestrate complex functions and interactions.

项目总结