Ultrasonic perfusion imaging of peripheral vascular disease

周围血管疾病的超声灌注成像

• 批准号: 10171895
• 负责人: Azra Alizad
• 金额: $ 67.85万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-05 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10171895
• 关键词: 3-Dimensional; Anatomy; Angiography; Animals; Ankle; Blood; Blood Vessels; Blood flow; Cardiovascular system; Clinic; Clinical; Clinical Research; Collaborations; Color; Complex; Computer software; Contrast Media; Data; Development; Diabetic mouse; Diagnosis; Diagnostic; Dimensions; Disease Progression; Doppler Ultrasound; Erythrocytes; Etiology; Evaluation; Event; Exercise Therapy; Failure; Family suidae; Frequencies; Gene Expression; Goals; Hindlimb; Human; Image; Imaging Device; Imaging Techniques; Injectable; Ischemia; Legal patent; Lesion; Life Style; Link; Measurement; Measures; Medical; Methods; Microspheres; Microvascular Dysfunction; Monitor; Mus; Muscle; Names; Nitric Oxide; Operative Surgical Procedures; Patient Monitoring; Patients; Pattern; Performance; Perfusion; Peripheral; Peripheral Vascular Diseases; Peripheral arterial disease; Physiologic pulse; Pre-Clinical Model; Production; Property; Radioactive; Recovery; Research; Research Design; Resolution; Risk; Sampling; Sensitivity and Specificity; Signal Transduction; Source; Staging; Surgeon; Symptoms; Techniques; Technology; Therapeutic; Time; Tissues; Translating; Translations; Ultrasonics; Update; Work; angiogenesis; base; blood perfusion; cardiovascular health; cell motility; claudication; cohort; cost; design; diabetic; diabetic patient; disability; disease diagnosis; experimental study; imaging approach; improved; in vivo; in vivo imaging; indexing; instrument; melanoma; mouse model; multimodality; muscle metabolism; novel; nuclear imaging; perfusion imaging; phantom model; porcine model; quantitative imaging; research study; response; simulation; spatiotemporal; success; tool; treatment response

Project Summary Our 4-yr project aims to develop a new ultrasonic Doppler method named Higher-Order Perfusion Estimation (HOPE) imaging. Applications enabled by this technology are routine assessments of microvascular disease progression in diabetic patients with symptoms of peripheral artery disease (PAD). Technical advances include new ultrasonic echo sampling and filtering techniques that significantly increase the sensitivity and specificity of standard sonographic instruments to spatially disorganized patterns of red-blood-cell movement without the addition of contrast media. Sensitivity to slowly perfusing blood is increased by transmitting a sparse regular sequence of Doppler pulses over long durations (1-10s). To counter a concomitant increase in clutter-signal power in perfusing-blood frequency channels, spatiotemporal echo acquisitions are first rearranged into 3-D data arrays and a 3-D singular-value decomposition (3D-SVD) clutter filter is formed for each experiment. Our approach now successfully separates weak perfusing-blood echoes from other echo-signal sources in the peripheral vasculature because of the typically narrow eigen-bandwidth of clutter echoes in peripheral muscle. Preliminary results using echo simulation, microchannel flow phantoms, and preclinical models of mouse ischemic hindlimb and melanoma lesions demonstrate that our method is very well designed for monitoring steady peripheral microvascular flow patterns using commercial ultrasonic instruments (with software updates). Four aims are proposed to demonstrate the utility of HOPE imaging (both power and color-flow) for measuring blood flow and perfusion. Aim 1 expands preliminary studies in mouse models to evaluate perfusion measurement sensitivity at 12-24 MHz in diabetic animals, and to uncover mechanisms of the angiogenic response of tissues to sudden ischemia. Aim 2 continues development of HOPE imaging by improving perfusing- blood echo sensitivity and clutter-filter performance under more general imaging conditions (viz., broad eigen- bandwidth clutter and 3-D spatially varying perfusion). Aim 3 focuses on a progressively ischemic pig model using 5-12 MHz HOPE imaging, MR angiography, and radioactive microsphere techniques to calibrate and compare HOPE imaging results with standard clinical approaches. Aim 4 is a 4-yr, 150-patient study designed to evaluate the diagnostic performance of HOPE imaging at assessing PAD. The overall project aims to develop existing ultrasonic instruments into highly-effect tools for evaluating microvascular changes leading to common disabilities and major cardiovascular events. The three in vivo studies proposed in this plan are designed to develop and evaluate HOPE imaging specifically for PAD diagnosis, staging, and therapeutic monitoring.

项目概要 我们为期 4 年的项目旨在开发一种新的超声多普勒方法，名为高阶灌注估计 （希望）成像。该技术的应用是微血管疾病的常规评估 具有外周动脉疾病（PAD）症状的糖尿病患者的进展。技术进步包括 新的超声回波采样和过滤技术显着提高了超声回波的灵敏度和特异性 标准超声仪器可以检测红细胞运动的空间紊乱模式，而无需 添加造影剂。通过传输稀疏的常规信号，可以增加对缓慢灌注血液的敏感性 长持续时间（1-10 秒）的多普勒脉冲序列。应对杂波信号的伴随增加 灌注血液频率通道中的功率，时空回波采集首先重新排列成 3D 数据 数组和 3-D 奇异值分解 (3D-SVD) 杂波滤波器为每个实验形成。我们的 现在，该方法成功地将微弱的灌注血液回波与其他回波信号源分开 由于外周肌肉中杂波回波的典型特征带宽较窄，因此外周血管系统受到影响。 使用回声模拟、微通道流模型和小鼠临床前模型得出的初步结果 缺血性后肢和黑色素瘤病变表明我们的方法非常适合监测 使用商用超声仪器（带软件更新）稳定外周微血管血流模式。 提出了四个目标来展示 HOPE 成像（功率和色流）在测量中的实用性 血流和灌注。目标 1 扩大小鼠模型的初步研究以评估灌注 在糖尿病动物中测量 12-24 MHz 的灵敏度，并揭示血管生成机制 组织对突然缺血的反应。目标 2 通过改善灌注继续发展 HOPE 成像 在更一般的成像条件下（即，广泛的特征值），血液回声灵敏度和杂波过滤器性能 带宽杂波和 3D 空间变化灌注）。目标 3 侧重于渐进性缺血猪模型 使用 5-12 MHz HOPE 成像、MR 血管造影和放射性微球技术来校准和 将 HOPE 成像结果与标准临床方法进行比较。目标 4 是一项为期 4 年、涉及 150 名患者的研究 评估 HOPE 成像在评估 PAD 时的诊断性能。项目总体目标是发展 将现有的超声仪器转变为高效工具，用于评估导致常见疾病的微血管变化 残疾和重大心血管事件。该计划中提出的三项体内研究旨在 开发和评估专门用于 PAD 诊断、分期和治疗监测的 HOPE 成像。