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Ultrasonic perfusion imaging of peripheral vascular disease

周围血管疾病的超声灌注成像

基本信息

批准号：
10413895
负责人：
Azra Alizad
金额：
$ 51.75万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-05 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10413895
关键词：
3-Dimensional Anatomy Angiography Animals Ankle Blood Blood Vessels Blood flow Cardiovascular system Clinic Clinical Clinical Research Collaborations Color Complex Computer software Contrast Media Data Development Diabetic mouse Diagnosis Diagnostic Dimensions Disease Progression Doppler Ultrasound Erythrocytes Etiology Evaluation Event Exercise Therapy Failure Family suidae Frequencies Gene Expression Goals Hindlimb Human Image Imaging Device Imaging Techniques Injectable Ischemia Legal patent Lesion Life Style Link Measurement Measures Medical Methods Microspheres Microvascular Dysfunction Monitor Mus Muscle Names Nitric Oxide Operative Surgical Procedures Patient Monitoring Patients Pattern Performance Perfusion Peripheral Peripheral Vascular Diseases Peripheral arterial disease Physiologic pulse Pre-Clinical Model Production Property Radioactive Recovery Research Research Design Resolution Risk Sampling Sensitivity and Specificity Signal Transduction Source Staging Surgeon Symptoms Techniques Technology Therapeutic Time Tissues Translating Translations Ultrasonics Update Work angiogenesis base blood perfusion cardiovascular health cell motility claudication cohort cost design diabetic diabetic patient diagnostic tool disability disease diagnosis experimental study imaging approach improved in vivo in vivo imaging indexing instrument melanoma mouse model multimodality muscle metabolism novel nuclear imaging perfusion imaging phantom model porcine model quantitative imaging research study response simulation spatiotemporal success tool treatment response

项目摘要

Project Summary Our 4-yr project aims to develop a new ultrasonic Doppler method named Higher-Order Perfusion Estimation (HOPE) imaging. Applications enabled by this technology are routine assessments of microvascular disease progression in diabetic patients with symptoms of peripheral artery disease (PAD). Technical advances include new ultrasonic echo sampling and filtering techniques that significantly increase the sensitivity and specificity of standard sonographic instruments to spatially disorganized patterns of red-blood-cell movement without the addition of contrast media. Sensitivity to slowly perfusing blood is increased by transmitting a sparse regular sequence of Doppler pulses over long durations (1-10s). To counter a concomitant increase in clutter-signal power in perfusing-blood frequency channels, spatiotemporal echo acquisitions are first rearranged into 3-D data arrays and a 3-D singular-value decomposition (3D-SVD) clutter filter is formed for each experiment. Our approach now successfully separates weak perfusing-blood echoes from other echo-signal sources in the peripheral vasculature because of the typically narrow eigen-bandwidth of clutter echoes in peripheral muscle. Preliminary results using echo simulation, microchannel flow phantoms, and preclinical models of mouse ischemic hindlimb and melanoma lesions demonstrate that our method is very well designed for monitoring steady peripheral microvascular flow patterns using commercial ultrasonic instruments (with software updates). Four aims are proposed to demonstrate the utility of HOPE imaging (both power and color-flow) for measuring blood flow and perfusion. Aim 1 expands preliminary studies in mouse models to evaluate perfusion measurement sensitivity at 12-24 MHz in diabetic animals, and to uncover mechanisms of the angiogenic response of tissues to sudden ischemia. Aim 2 continues development of HOPE imaging by improving perfusing- blood echo sensitivity and clutter-filter performance under more general imaging conditions (viz., broad eigen- bandwidth clutter and 3-D spatially varying perfusion). Aim 3 focuses on a progressively ischemic pig model using 5-12 MHz HOPE imaging, MR angiography, and radioactive microsphere techniques to calibrate and compare HOPE imaging results with standard clinical approaches. Aim 4 is a 4-yr, 150-patient study designed to evaluate the diagnostic performance of HOPE imaging at assessing PAD. The overall project aims to develop existing ultrasonic instruments into highly-effect tools for evaluating microvascular changes leading to common disabilities and major cardiovascular events. The three in vivo studies proposed in this plan are designed to develop and evaluate HOPE imaging specifically for PAD diagnosis, staging, and therapeutic monitoring.

项目摘要我们的四年计划旨在发展一种新的超声多普勒方法，称为高阶灌注估计（HOPE）成像。该技术的应用是微血管疾病的常规评估伴有外周动脉疾病（PAD）症状的糖尿病患者的进展。技术进步包括新的超声回波采样和滤波技术，显著提高了灵敏度和特异性，标准超声仪器对红细胞运动的空间无序模式进行检查，添加造影剂。对缓慢灌注的血液的敏感性通过传输稀疏的规则信号而增加。长持续时间（1- 10秒）的多普勒脉冲序列。为了抵消伴随的杂波信号的增加，在灌注血液频率通道中的功率，首先将时空回波采集重新排列成3-D数据阵列和3-D奇异值分解（3D-SVD）杂波滤波器形成的每个实验。我们现在，这种方法成功地将微弱的灌注血液回波与其他回波信号源分离开来，由于外周肌肉中杂波回波的特征带宽通常较窄，使用回声模拟、微通道流动模型和小鼠临床前模型的初步结果缺血性后肢和黑色素瘤病变表明，我们的方法是非常好的监测设计，使用商业超声仪器（软件更新）稳定外周微血管血流模式。提出了四个目标，以证明HOPE成像（功率和彩色流）用于测量的实用性血流和灌注。目的1扩展了小鼠模型的初步研究，以评估灌注在糖尿病动物中在12-24 MHz下的测量灵敏度，并揭示血管生成的机制。组织对突然缺血的反应。目标2通过改善灌注继续开发HOPE成像- 在更一般的成像条件下的血液回波灵敏度和杂波滤波器性能（即，宽本征值带宽杂波和3-D空间变化灌注）。目的3关注进行性缺血猪模型使用5-12 MHz HOPE成像、MR血管造影和放射性微球技术进行校准，将HOPE成像结果与标准临床方法进行比较。Aim 4是一项为期4年、150例患者的研究，评价HOPE成像在评估PAD方面的诊断性能。整个项目旨在开发将现有的超声仪器转变为用于评估微血管变化的高效工具，残疾和重大心血管事件。本计划中提出的三项体内研究旨在开发和评估HOPE成像，专门用于PAD诊断、分期和治疗监测。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Monitoring Muscle Perfusion in Rodents During Short-Term Ischemia Using Power Doppler Ultrasound.

使用能量多普勒超声监测啮齿类动物短期缺血期间的肌肉灌注。

DOI：
10.1016/j.ultrasmedbio.2023.02.013
发表时间：
2023
期刊：
Ultrasound in medicine & biology
影响因子：
2.9
作者：
Babaei,Somaye;Dai,Bingze;Abbey,CraigK;Ambreen,Yamenah;Dobrucki,WawrzyniecL;Insana,MichaelF
通讯作者：
Insana,MichaelF

Contrast-free ultrasound imaging for blood flow assessment of the lower limb in patients with peripheral arterial disease: a feasibility study.