Discipline D Special Project - Track 3: Method Development and Validation
Discipline D Special Project - Track 3: Method Development and Validation
批准号:
10173388
负责人:
Yong Liu
金额:
$3.5万
依托单位国家:
美国
项目类别:
财政年份:
2020
资助国家:
美国
项目状态:
未结题
起止时间:
2020-09-01 至 2025-06-30
中文摘要
PAR-20-105:学科D -特殊项目-专题3:方法验证
英文摘要
PAR-20-105: Discipline D – Special Projects - Track 3: Method Validation
Project Summary
The Washington State Department of Agriculture (WSDA) Food Safety and Consumer Services
(FS&CS) Laboratory is the State’s Primary Regulatory Human Food and Animal Feed Safety
Laboratory. We seek 5 years of funding through the FDA Laboratory Flexible Funding Model (LFFM)
Cooperative Agreement Program’s Discipline D Special Projects Track 3, Method Development and
Validation. We plan to utilize the funding to conduct preliminary, short-term or exploratory
investigations that focus on the feasibility of a new method and/or technology. We propose to be part
of the multi-laboratory validation of a qPCR screening method for Salmonella in foods in Year-1.
The FS&CS Laboratory has two decades of recent work developing new methods and validating
the methods. From 2001-2013 the laboratory was one of 8 selected state agriculture laboratories
for support under the USDA Microbiological Data Program. The purpose of this program was to
develop and validate testing methods for fresh produce. Another purpose was to understand the
frequency of detected pathogens in various produce items.
The Laboratory has extensive experience working with complicated matrices that present
challenges to modern technical testing platforms. For instance, sprouts are difficult to test since
there is a lot of interference with real-time PCR.
Each method for Salmonella, Listeria, E.coli O157:H7, non-O157 E.coli (STEC), Shigella had to
be adapted to testing produce. This required years of work to discover, verify and validate the
methods. Each matrix then had to be re-verified and validated.
The Laboratory has worked under the FDA FERN MCAP for over 10 years. A primary goal of this
cooperative agreement is to develop testing methods on newer test platforms and then validate
those methods. In addition, we have a long history of collaborating with private companies who
manufacture test kits and analytical equipment, in order to get some of these technologies
approved by AOAC and available for our commercial use.
The Laboratory utilizes the FDA Guideline for the Validation of Analytical Methods.
If our Track 3 proposal is accepted, we are willing and able to do the following:
1. Participate the multi-laboratory validation of a qPCR screening method for Salmonella in foods
by following FDA guidelines for method validation.
Washington State Department of Agriculture, Food Safety and Consumer Services Laboratory
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Discipline A Microbiology - Track 5: Capacity and Capability Development
-
批准号:10445631
-
项目类别:
-
资助金额:$7.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline A Microbiology-Track 4: WGS
-
批准号:10445630
-
项目类别:
-
资助金额:$3.0万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline A - Microbiology - Analytical Track 2: Human Food Product Testing
-
批准号:10173382
-
项目类别:
-
资助金额:$16.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline A Microbiology - Track 5: Capacity and Capability Development
-
批准号:10173385
-
项目类别:
-
资助金额:$7.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline A Microbiology-Track 4: WGS
-
批准号:10173384
-
项目类别:
-
资助金额:$3.0万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Enhancing the Capacity and Capability of Washington State Human and Animal Food Testing Laboratory in Support of an Integrated Food Safety System
-
批准号:10173380
-
项目类别:
-
资助金额:$52.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline A Microbiology Track 3: Animal Food Product Testing
-
批准号:10173383
-
项目类别:
-
资助金额:$16.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Enhancing the Capacity and Capability of Washington State Human and Animal Food Testing Laboratory in Support of an Integrated Food Safety System
-
批准号:10256672
-
项目类别:
-
资助金额:$38.3万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline D Special Project - Track 2: NFSDX and ORAPP Integration
-
批准号:10173387
-
项目类别:
-
资助金额:$2.0万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Enhancing the Capacity and Capability of Washington State Human and Animal Food Testing Laboratory in Support of an Integrated Food Safety System
-
批准号:10448315
-
项目类别:
-
资助金额:$39.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Enhancing the Capacity and Capability of Washington State Human and Animal Food Testing Laboratory in Support of an Integrated Food Safety System
-
批准号:10461359
-
项目类别:
-
资助金额:$18.02万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline A Microbiology Track 3: Animal Food Product Testing
-
批准号:10445629
-
项目类别:
-
资助金额:$10.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline D Special Project - Track 1: Sample Collection
-
批准号:10445632
-
项目类别:
-
资助金额:$3.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Enhancing the Capacity and Capability of Washington State Human and Animal Food Testing Laboratory in Support of an Integrated Food Safety System
-
批准号:10663228
-
项目类别:
-
资助金额:$38.0万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline D Special Project - Track 1: Sample Collection
-
批准号:10173386
-
项目类别:
-
资助金额:$3.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline A - Microbiology - Analytical Track 2: Human Food Product Testing
-
批准号:10445628
-
项目类别:
-
资助金额:$10.3万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Discipline D Special Project - Track 3: Method Development and Validation
-
批准号:10445633
-
项目类别:
-
资助金额:$3.5万
-
财政年份:2020
-
负责人:Yong Liu
-
依托单位:
Maintenance and Enhancement of ISO/IEC 17025 Accreditation and Whole Genome Sequencing for State Food Testing Laboratories
-
批准号:9930496
-
项目类别:
-
资助金额:$14.5万
-
财政年份:2019
-
负责人:Yong Liu
-
依托单位:
FDA FERN Microbiological Cooperative Agreement Continuation Program (U18)
-
批准号:9767558
-
项目类别:
-
资助金额:$21.4万
-
财政年份:2015
-
负责人:Yong Liu
-
依托单位:
ISO / IEC 17025:2005 Accreditation for State Food Testing Laboratories (U18)
-
批准号:8914428
-
项目类别:
-
资助金额:$15.0万
-
财政年份:2012
-
负责人:Yong Liu
-
依托单位:
国内基金
海外基金
非阶化Hamiltonial型和Special型李代数的表示
-
批准号:10701002
-
项目类别:青年科学基金项目
-
资助金额:15.0万元
-
批准年份:2007
-
负责人:赵玉凤
-
依托单位: