Enhancing the Capacity and Capability of Human Food and Environmental Testing Laboratory at the Texas Department of State Health Services: Special Projects Method Development and Method Validation

增强德克萨斯州卫生服务部人类食品和环境检测实验室的能力和能力：特别项目方法开发和方法验证

• 批准号: 10174182
• 负责人: Rachel C. Lee
• 金额: $ 4.07万
• 依托单位: TEXAS STATE DEPT OF HEALTH SERVICES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10174182
• 关键词: Enhancing; Capacity; Capability; Human; Food

Project Summary: Discipline D Special Projects Track 3- Method Development/Validation, Multi-laboratory Validation of a qPCR Screening Method for Salmonella in Foods The Texas Department of State Health Services (DSHS) Laboratory Services Section (LSS) has been a member of the Food and Drug Administration (FDA) Food Emergency Response Network (FERN) and the Genome Trakr Network and a member of the Centers for Disease Control and Prevention (CDC) Laboratory Response Network (LRN), PulseNet, and CaliciNet. LSS is also ISO/IEC 17025 accredited for ten food tests that cover Salmonella, Listeria, E. coli O157:H7, Campylobacter, and the Big “6” non-O157 STEC species, receiving and analyzing approximately 2,500 food samples per year. As a part of an integrated food safety system, the LSS is committed to facilitating and improving food testing surveillance, inspection, and investigation in the State of Texas. The goal of this project is to participate in preliminary, short-term, or exploratory multi-laboratory validation (MLV) studies that focus on testing the feasibility of the FDA qPCR Screening Method for identifying Salmonella in foods. The LSS Advanced Molecular Microbiology group (AMMG) will follow the most up-to-date FDA protocols and processes as specified by the FDA. With two ABI 7500 Fast Real-Time PCR systems, the AMMG can test up to 1000 Salmonella food and environmental samples per year in addition to all routine and maintenance activities and does not expect any issues in performance capacity to participate in the MLV studies. With the funds provided from this grant, LSS will purchase supplies needed to participate in the MLV studies, train staff, perform the studies, participate in meetings and activities related to the studies, and maintain facilities and personnel necessary to complete the work proposed. Funding provided by this cooperative agreement will serve to strengthen our states commitment to a safe food supply and improve the ability of DSHS to respond to a large-scale threat to the nation's food supply.

项目概要：D学科特别项目 途径3-方法开发/验证，qPCR筛查的多实验室验证 食品中沙门氏菌的检测方法 德克萨斯州卫生服务部（DSHS）实验室服务科（LSS） 一直是美国食品和药物管理局（FDA）食品应急响应的成员 网络（FERN）和基因组Trakr网络和疾病中心的成员 控制和预防（CDC）实验室响应网络（LRN）、PulseNet和CaliciNet。 LSS还通过了ISO/IEC 17025认证，可进行十项食品检测，包括沙门氏菌、李斯特菌、大肠杆菌和沙门氏菌。杆菌 O 157：H7、弯曲杆菌、大“6”非O 157 STEC菌种，接收分析 每年约2,500个食品样本。作为综合食品安全体系的一部分， LSS致力于促进和改善食品检测监督，检查， 在德克萨斯州的调查。 本项目的目标是参与初步的、短期的、或探索性的多实验室 验证（MLV）研究，重点检测FDA qPCR筛选方法的可行性 用于识别食物中的沙门氏菌。LSS Advanced Molecular Microbiology Group（AMMG） 将遵循FDA规定的最新FDA方案和流程。具有两 ABI 7500快速实时PCR系统，AMMG可以检测多达1000种沙门氏菌食品， 除了所有日常和维护活动外，每年还需要收集环境样本， 预期参与MLV研究的性能不会有任何问题。与各基金 根据该补助金，LSS将购买参与MLV研究所需的用品， 培训工作人员，开展研究，参加与研究有关的会议和活动，以及 维护完成拟议工作所需的设施和人员。 这项合作协定提供的资金将有助于加强我们各国的承诺 安全的食品供应，并提高DSHS应对大规模威胁的能力。 国家的粮食供应。