Enhancing the Capacity and Capability of Human Food and Environmental Testing Laboratory at the Texas Department of State Health Services: Microbiology Whole Genome Sequencing

项目摘要 学科A微生物学，轨道4全基因组测序 德克萨斯州卫生服务部实验室服务科 (LSS)是美国最大的公共卫生实验室之一。作为成员之一 食品和药物管理局(FDA)食品应急网络(FERN)， GenomeTrakr网络和疾病控制与预防中心 PulseNet，它致力于改善食品检测的监督、检查和 德克萨斯州的调查。 在德克萨斯州进行食源性病原体检测对该州来说很重要， 地区和国家，部分原因是它与墨西哥广阔的边界，那里的农业 产品进入该国；LSS识别数百种食源性细菌病原体 每年都会聚集在一起。通过使用全基因组测序(WGS)，LSS Advanced 分子微生物学集团计划对至少400株沙门氏菌、李斯特氏菌进行测序 单核细胞增多症、产志贺毒素大肠杆菌(STEC)和其他相关细菌分离株 从食物和环境样本中提取。这些分离物的潜在来源包括LSS 内部食品检测实验室，FDA国家抗菌素耐药性监测 系统(NARMS)肉类计划，美国农业部，以及具有测试和研究的大学 实验室。 LSS是国际标准化组织/国际电工委员会17025认证的符合标准的实验室 监管测试方法和报告要求。使用5个MiSeq测序器， LSS具有每周对约250-300个分离物进行测序的能力和能力。LSS当前 向CDC PulseNet数据库提交包括李斯特菌在内的生物体的序列， 沙门氏菌、大肠杆菌O157和非O157志贺氏菌、志贺氏菌、弯曲杆菌、弧菌 副溶血性弧菌和霍乱弧菌。 LSS致力于与FDA的伙伴关系，并将继续参与 在Fern和GenomeTrakr活动中，包括但不限于会议、多实验室 验证研究，以及对其他州立实验室的培训和指导。在…的支持下 这笔拨款，将能够加强对食品检测紧急情况的反应和 改进食源性疾病调查，确保人类食品和食品安全 产品销往德克萨斯州和全国。

Project Summary Discipline A Microbiology, Track 4 Whole Genome Sequencing The Texas Department of State Health Services (DSHS) Laboratory Services Section (LSS) is one of the largest public health laboratories in the United States. As a member of the Food and Drug Administration (FDA) Food Emergency Response Network (FERN), GenomeTrakr Network and the Centers for Disease Control and Prevention (CDC) PulseNet, it is committed to improving food testing surveillance, inspection, and investigation in the State of Texas. Detection of foodborne pathogens performed in Texas is important for the state, region, and nation due in part to its extensive border with Mexico where agricultural products enter the country; LSS identifies hundreds of foodborne bacterial pathogen clusters each year. By using Whole Genome Sequencing (WGS) the LSS Advanced Molecular Microbiology Group plans to sequence at least 400 Salmonella, Listeria monocytogenes, Shiga toxin-producing E. coli (STEC) and other related bacterial isolates from food and environmental samples. Potential sources of the isolates include the LSS internal food testing laboratory, the FDA National Antimicrobial Resistant Monitoring System (NARMS) meat program, USDA, and universities with testing and research laboratories. The LSS an ISO/IEC 17025 accredited laboratory that adheres to standard regulatory testing methodologies and reporting requirements. With 5 MiSeq sequencers, LSS has the capability and capacity to sequence ~250-300 isolates per week. LSS currently submits sequences to the CDC PulseNet database for organisms including Listeria, Salmonella, E. coli O157 and non-O157 STEC, Shigella, Campylobacter, Vibrio parahaemolyticus, and Vibrio cholera. The LSS is committed to its partnership with the FDA and will continue to participate in FERN and GenomeTrakr activities that include but are not limited to meetings, multi-lab validation studies, and training and mentoring to other state laboratories. With the support of this grant, DSHS LSS will be able to enhance its response to food testing emergencies and improve foodborne illness investigations to ensure the safety of human food and food products in Texas and the nation.