Enhancing the Capacity and Capability of Human Food and Environmental Testing Laboratory at the Texas Department of State Health Services: Microbiology Whole Genome Sequencing

项目摘要 学科A微生物学，轨道4全基因组测序 德克萨斯州卫生服务部（DSHS）实验室服务科 (LSS)是美国最大的公共卫生实验室之一人格的范围 美国食品药品监督管理局（FDA）食品紧急反应网络（FERN）， GenomeTrakr网络和疾病控制和预防中心（CDC） PulseNet致力于改善食品检测监督、检查和 在德克萨斯州的调查。 在德克萨斯州进行的食源性病原体检测对该州很重要， 部分原因是其与墨西哥的广泛边界， 产品进入该国; LSS确定了数百种食源性细菌病原体 每年的集群。通过使用全基因组测序（WGS），LSS高级 分子微生物学小组计划对至少400种沙门氏菌和李斯特菌进行测序 单核细胞增多症、产滋贺毒素E.大肠杆菌（STEC）和其他相关细菌分离株 从食物和环境样本中提取。分离株的潜在来源包括LSS 内部食品测试实验室，FDA国家抗菌素耐药性监测 系统（NARMS）肉类计划，美国农业部和大学的测试和研究 laboratories. LSS是ISO/IEC 17025认证的实验室， 监管测试方法和报告要求。有了5个MiSeq测序仪， LSS具有每周测序约250-300个分离株的能力。LSS目前 向CDC PulseNet数据库提交包括李斯特菌在内的生物体的序列， 沙门氏菌属、E.大肠杆菌O 157和非O 157 STEC、志贺氏菌、弯曲杆菌、弧菌 副溶血性弧菌和霍乱弧菌。 LSS致力于与FDA的伙伴关系，并将继续参与 在FERN和GenomeTrakr活动中，包括但不限于会议、多实验室 验证研究以及对其他国家实验室的培训和指导。的支持下 这笔赠款，DSHS LSS将能够加强其对食品检测紧急情况的反应， 加强食源性疾病调查，确保人类食品和食品安全 产品在德州和全国

Project Summary Discipline A Microbiology, Track 4 Whole Genome Sequencing The Texas Department of State Health Services (DSHS) Laboratory Services Section (LSS) is one of the largest public health laboratories in the United States. As a member of the Food and Drug Administration (FDA) Food Emergency Response Network (FERN), GenomeTrakr Network and the Centers for Disease Control and Prevention (CDC) PulseNet, it is committed to improving food testing surveillance, inspection, and investigation in the State of Texas. Detection of foodborne pathogens performed in Texas is important for the state, region, and nation due in part to its extensive border with Mexico where agricultural products enter the country; LSS identifies hundreds of foodborne bacterial pathogen clusters each year. By using Whole Genome Sequencing (WGS) the LSS Advanced Molecular Microbiology Group plans to sequence at least 400 Salmonella, Listeria monocytogenes, Shiga toxin-producing E. coli (STEC) and other related bacterial isolates from food and environmental samples. Potential sources of the isolates include the LSS internal food testing laboratory, the FDA National Antimicrobial Resistant Monitoring System (NARMS) meat program, USDA, and universities with testing and research laboratories. The LSS an ISO/IEC 17025 accredited laboratory that adheres to standard regulatory testing methodologies and reporting requirements. With 5 MiSeq sequencers, LSS has the capability and capacity to sequence ~250-300 isolates per week. LSS currently submits sequences to the CDC PulseNet database for organisms including Listeria, Salmonella, E. coli O157 and non-O157 STEC, Shigella, Campylobacter, Vibrio parahaemolyticus, and Vibrio cholera. The LSS is committed to its partnership with the FDA and will continue to participate in FERN and GenomeTrakr activities that include but are not limited to meetings, multi-lab validation studies, and training and mentoring to other state laboratories. With the support of this grant, DSHS LSS will be able to enhance its response to food testing emergencies and improve foodborne illness investigations to ensure the safety of human food and food products in Texas and the nation.