Enhancing the Capacity and Capability of Human Food and Environmental Testing Laboratory at the Texas Department of State Health Services: Microbiology Capability/Capacity Development

增强德克萨斯州卫生服务部人类食品和环境检测实验室的能力和能力：微生物学能力/能力发展

• 批准号: 10174176
• 负责人: Rachel C. Lee
• 金额: $ 12.13万
• 依托单位: TEXAS STATE DEPT OF HEALTH SERVICES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10174176
• 关键词: Enhancing; Capacity; Capability; Human; Food

Project Summary Discipline A Microbiology, Track 5- Cyclospora Capability/Capacity Development The Texas Department of State Health Services (DSHS) Laboratory Services Section (LSS) works in conjunction with the Food and Drug Administration (FDA), the Centers for Disease Control and Prevention (CDC), and the state regulatory body, DSHS Consumer Protection Division (CPD), to ensure food and food products in Texas are safe. Cyclosporiasis, an intestinal illness caused by Cyclospora cayetanensis has increasingly been the source of outbreaks and significant sporadic illness in Texas. For this reason, DSHS LSS and CPD are interested in testing human food products for the presence of Cyclospora cayetanensis using real-time PCR for rapid and sensitive detection. The long-term goal is for this process to be a part of a routine surveillance test on food and environmental samples. An implementation plan has been developed to purchase equipment, train staff, modify the laboratory information management system, evaluate and validate testing methodology, and demonstrate competency through proficiency testing. DSHS LSS will then work with CPD to add Cyclospora cayetanensis to the Sample Collection and Analysis Project of the Microbiology Human Food Testing Track. LSS will also work with the DSHS Infectious Disease and Control Unit and the DSHS Texas Rapid Response Team during outbreak investigations of Cyclospora cayetanensis in human food. The LSS Consumer Microbiology Team (CMT) and Molecular Biology Team (MBT) will be performing procedures in methods following the FDA Bacteriological Analytical Manual 19b: Molecular Detection of Cyclospora cayetanensis in Fresh Produce Using Real-Time PCR. LSS will continue to follow FDA guidance for proper sampling and laboratory data documentation and participate in annual proficiency tests (PT) for work performed under this project. LSS will stay committed to its 20.88 agreement with the FDA and will attend the annual LFFM Face-to- Face meeting to ensure continued collaboration between LSS and FDA. This project will strengthen and improve the collaboration of surveillance testing activities already established between the FDA and our state and local partners improving the ability of DSHS to respond to a large-scale threat to the nation's food supply.

项目摘要 学科A微生物学，轨道5--环孢子菌能力/能力发展 德克萨斯州卫生服务部实验室服务科(LSS) 与食品和药物管理局(FDA)、疾病中心 控制和预防(CDC)和州监管机构DSHS消费者保护 部门(CPD)，以确保德克萨斯州的食品和食品安全。环孢子虫病 由卡氏环孢菌引起的肠道疾病越来越多地成为 在德克萨斯州爆发和重大的零星疾病。因此，DSHS LSS和CPD是 有兴趣检测人类食品中是否存在卡耶坦环孢菌 实时荧光聚合酶链式反应用于快速、灵敏的检测。长期目标是使这一进程成为一个 这是对食品和环境样本进行的常规监测测试的一部分。 已经制定了一项实施计划，以购买设备、培训工作人员、修改 实验室信息管理系统，评估和验证测试方法，以及 通过能力测试证明能力。然后，DHS LSS将与CPD合作，以添加 卡氏环孢子菌在微生物学样品采集与分析项目中的应用 人类食品检测赛道。LSS还将与DSHS传染病和控制中心合作 在环孢子虫暴发调查期间与德克萨斯州疾病安全和公众服务局快速反应小组 人类食物中的卡耶坦。 LSS消费者微生物学团队(CMT)和分子生物学团队(MBT)将 按照FDA细菌分析手册19b的方法执行程序： 实时荧光定量聚合酶链式反应检测生鲜农产品中卡氏环孢霉LSS 将继续遵循FDA的指导方针进行适当的采样和实验室数据记录 并参加每年一次的能力测试(PT)，以测试在该项目下完成的工作。LSS将会 继续致力于其与FDA的20.88协议，并将参加年度LFFM面对面 面对面会议，以确保LSS和FDA之间的持续合作。 该项目将加强和改进监督检测活动的协作 FDA与我们的州和当地合作伙伴之间已经建立了合作伙伴关系，以提高 对国家粮食供应的大规模威胁作出反应。