Enhancing the Capacity and Capability of Human Food and Environmental Testing Laboratory at the Texas Department of State Health Services: Microbiology Capability/Capacity Development

项目摘要 学科A微生物学，轨道5-环孢菌能力/能力发展 德克萨斯州卫生服务部（DSHS）实验室服务科（LSS） 与食品和药物管理局（FDA），疾病中心 控制和预防（CDC），以及国家监管机构，DSHS消费者保护 部门（CPD），以确保食品和食品产品在得克萨斯州是安全的。环孢霉属 由环孢子虫引起的肠道疾病越来越多地成为 疫情和重大零星疾病在得克萨斯州。因此，DSHS LSS和CPD 感兴趣的是测试人类食品中是否存在环孢子虫cayetanensis， 实时荧光定量PCR检测快速、灵敏。长期目标是使这一进程成为一个 食物及环境样本的例行监察测试的一部分。 已经制定了一项执行计划，以购买设备、培训工作人员、修改 实验室信息管理系统，评估和验证测试方法，以及 通过能力测试证明能力。DSHS LSS将与CPD合作， 环孢子虫对微生物学样品采集与分析项目的影响 人类食品测试跟踪。LSS还将与DSHS传染病和控制部门合作， 在环孢子虫爆发调查期间， 人类食物中的卡耶丹 LSS消费者微生物学团队（CMT）和分子生物学团队（MBT）将 按照FDA细菌学分析手册19 b： 实时荧光定量PCR技术检测新鲜农产品中的环孢子虫LSS 将继续遵循FDA的指导，进行适当的采样和实验室数据记录 并参加本项目下所做工作的年度能力测试。LSS将 继续致力于其与FDA的20.88协议，并将参加年度LFFM面对面， 面对面会议以确保LSS和FDA之间的持续合作。 该项目将加强和改善监测检测活动的协作 已经建立了FDA和我们的国家和地方合作伙伴之间的能力， DSHS是为了应对对国家粮食供应的大规模威胁。

Project Summary Discipline A Microbiology, Track 5- Cyclospora Capability/Capacity Development The Texas Department of State Health Services (DSHS) Laboratory Services Section (LSS) works in conjunction with the Food and Drug Administration (FDA), the Centers for Disease Control and Prevention (CDC), and the state regulatory body, DSHS Consumer Protection Division (CPD), to ensure food and food products in Texas are safe. Cyclosporiasis, an intestinal illness caused by Cyclospora cayetanensis has increasingly been the source of outbreaks and significant sporadic illness in Texas. For this reason, DSHS LSS and CPD are interested in testing human food products for the presence of Cyclospora cayetanensis using real-time PCR for rapid and sensitive detection. The long-term goal is for this process to be a part of a routine surveillance test on food and environmental samples. An implementation plan has been developed to purchase equipment, train staff, modify the laboratory information management system, evaluate and validate testing methodology, and demonstrate competency through proficiency testing. DSHS LSS will then work with CPD to add Cyclospora cayetanensis to the Sample Collection and Analysis Project of the Microbiology Human Food Testing Track. LSS will also work with the DSHS Infectious Disease and Control Unit and the DSHS Texas Rapid Response Team during outbreak investigations of Cyclospora cayetanensis in human food. The LSS Consumer Microbiology Team (CMT) and Molecular Biology Team (MBT) will be performing procedures in methods following the FDA Bacteriological Analytical Manual 19b: Molecular Detection of Cyclospora cayetanensis in Fresh Produce Using Real-Time PCR. LSS will continue to follow FDA guidance for proper sampling and laboratory data documentation and participate in annual proficiency tests (PT) for work performed under this project. LSS will stay committed to its 20.88 agreement with the FDA and will attend the annual LFFM Face-to- Face meeting to ensure continued collaboration between LSS and FDA. This project will strengthen and improve the collaboration of surveillance testing activities already established between the FDA and our state and local partners improving the ability of DSHS to respond to a large-scale threat to the nation's food supply.