ShEEP Request for a High-Content Screening (HCS) Platform

ShEEP 请求建立高内涵筛选 (HCS) 平台

• 批准号: 10175276
• 负责人: Dandan Sun
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175276
• 关键词: Area; Biological; Biological Assay; Cell Cycle Kinetics; Cells; Collaborations; Color; Communicable Diseases; Communities; Computer software; Cultured Cells; Data; Development; Diagnosis; Discrimination; Disease; Equipment; Extramural Activities; Funding; Future; General Population; Goals; Histology; Image; Imaging Device; Incubators; Inflammatory; Investments; Laboratories; Laboratory Research; Lasers; Lung diseases; Maintenance; Malignant Neoplasms; Measures; Medical; Medical Research; Mentors; Microscope; Mus; Neurodegenerative Disorders; Organ Culture Techniques; Organoids; Physiology; Productivity; Regenerative Medicine; Research; Research Personnel; Resolution; Sampling; Scientist; Secure; Semiconductors; Sepsis; Sheep; Site; Slice; Speed; Strategic Planning; Stroke; System; Thick; Time; Tissues; Traumatic Brain Injury; Universities; Veterans; base; bone; career; chromophore; data acquisition; equipment acquisition; flexibility; fluorescence imaging; human tissue; improved; instrument; intelligent algorithm; interest; laboratory equipment; laboratory facility; lens; live cell imaging; metal oxide; nervous system disorder; programs; quantum; research facility; screening; solid state; success; synergism; tissue culture; tool; wound healing

Research at the VA Pittsburgh encompasses a broad spectrum of medical fields and is aimed at improving diagnosis and treatment of a wide range of disorders that are highly prevalent in Veterans, as well as in the general population, and that represent a priority for ORD funding and strategic planning. Major areas covered by research at the VA are neurological disorders (stroke, traumatic brain injuries, neurodegenerative disorders), pulmonary diseases, cancer, inflammatory disorders, infectious diseases, sepsis, wound healing, regenerative medicine. A common thread in the Medical Research Merit Programs is the use of cell, organ culture systems and mouse/human tissues that benefit from imaging-based analyses. In this proposal we aim to obtain funds to purchase the laser based, confocal equipped CELLINSIGHT CX7 High Content Screening Platform in order to meet the growing needs for more advanced and versatile imaging tools by the scientific community at the VA Pittsburgh. The funded research programs of these investigators require many capabilities: fixed imaging of a multitude of targets simultaneously, high-resolution discrimination of subcellular localization, cultured cells, slice tissue cultures and live cell imaging in combination with robust, reliable and versatile quantitative analyses. We identified the laser based, confocal equipped CELLINSIGHT CX7 High Content Screening Platform as a single instrument that provides the multiplexing capability using tissues, cells, organoids and intelligent algorithms for quantitative analyses. This instrument is not available either at the VA Pittsburgh or at our affiliate, the University of Pittsburgh. The acquisition of this equipment is timely with the recent opening of the VA’s state-of-the-art research facility where the equipment will be housed. A rational administrative plan has been developed to effectively manage efficient use of the platform and provide oversight for maintenance. The overall impact of the acquisition of this equipment is broad considering that 13 established investigators will be regular users of the microscope and additional PIs are expected to become users in the future as part of a growing cadre of newly VA funded scientists. By enabling VA investigators to obtain high quality data, we anticipate a seamless exchange of scientific information and an enhanced opportunity for collaborative projects between VA investigators and the University of Pittsburgh. Furthermore, two of the co-investigators are CDA awardees. The mentored stage of a clinician scientist is most critical for the successful development of their academic career. This new HCS platform will enhance their productivity by offering greater imaging quality and speed of data acquisition and help them transition from mentored to independent investigator status. Finally, the availability of this platform in the VA laboratories is expected to create opportunities for collaborations and synergize projects that have complementary focus.

VA匹兹堡的研究涵盖了广泛的医学领域，旨在 改善对退伍军人中普遍存在的各种疾病的诊断和治疗， 与普通人群一样，这是ORD资金和战略规划的优先事项。大领域 退伍军人事务部的研究所涵盖的是神经系统疾病（中风、创伤性脑损伤、神经退行性疾病）， 疾病），肺部疾病，癌症，炎性疾病，感染性疾病，败血症，伤口愈合， 再生医学医学研究优秀项目的一个共同点是使用细胞、器官 培养系统和小鼠/人体组织，受益于基于成像的分析。 在本提案中，我们的目标是获得资金，以购买基于激光的共焦装备CELLINSIGHT CX 7高内容筛选平台，以满足日益增长的对更高级和多功能的需求 匹兹堡退伍军人管理局科学界的成像工具。这些资助的研究项目 调查人员需要许多能力：同时对多个目标进行固定成像，高分辨率 区分亚细胞定位、培养细胞、切片组织培养和活细胞成像， 结合强大、可靠和通用的定量分析。我们发现了基于激光的共焦 配备CELLINSIGHT CX 7高含量筛选平台作为单一仪器， 使用组织、细胞、类器官和智能算法进行定量分析的多路复用能力。这 仪器是不可用的，无论是在VA匹兹堡或在我们的附属机构，匹兹堡大学。 随着退伍军人事务部最近开始进行最先进的研究， 设备将被安置的设施。制定了合理的行政计划， 管理平台的有效使用并提供维护监督。的总体影响 考虑到13名常设调查员将经常使用这台设备， 显微镜和其他PI预计将成为用户在未来的一部分，越来越多的干部，新的 资助的科学家。通过使VA调查人员能够获得高质量的数据，我们预计 交流科学信息，增加VA之间合作项目的机会 调查人员和匹兹堡大学。此外，两名共同调查员是CDA获奖者。的 临床科学家的指导阶段是其学术生涯成功发展的关键。 这个新的HCS平台将通过提供更高的成像质量和数据速度来提高他们的工作效率 收购并帮助他们从辅导员身份过渡到独立调查员身份。最后， VA实验室的这个平台有望为合作和协同项目创造机会 有互补的焦点。