ShEEP Request for a High-Content Screening (HCS) Platform

ShEEP 请求建立高内涵筛选 (HCS) 平台

• 批准号: 10175276
• 负责人: Dandan Sun
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175276
• 关键词: Area; Biological; Biological Assay; Cell Cycle Kinetics; Cells; Collaborations; Color; Communicable Diseases; Communities; Computer software; Cultured Cells; Data; Development; Diagnosis; Discrimination; Disease; Equipment; Extramural Activities; Funding; Future; General Population; Goals; Histology; Image; Imaging Device; Incubators; Inflammatory; Investments; Laboratories; Laboratory Research; Lasers; Lung diseases; Maintenance; Malignant Neoplasms; Measures; Medical; Medical Research; Mentors; Microscope; Mus; Neurodegenerative Disorders; Organ Culture Techniques; Organoids; Physiology; Productivity; Regenerative Medicine; Research; Research Personnel; Resolution; Sampling; Scientist; Secure; Semiconductors; Sepsis; Sheep; Site; Slice; Speed; Strategic Planning; Stroke; System; Thick; Time; Tissues; Traumatic Brain Injury; Universities; Veterans; base; bone; career; chromophore; data acquisition; equipment acquisition; flexibility; fluorescence imaging; human tissue; improved; instrument; intelligent algorithm; interest; laboratory equipment; laboratory facility; lens; live cell imaging; metal oxide; nervous system disorder; programs; quantum; research facility; screening; solid state; success; synergism; tissue culture; tool; wound healing

Research at the VA Pittsburgh encompasses a broad spectrum of medical fields and is aimed at improving diagnosis and treatment of a wide range of disorders that are highly prevalent in Veterans, as well as in the general population, and that represent a priority for ORD funding and strategic planning. Major areas covered by research at the VA are neurological disorders (stroke, traumatic brain injuries, neurodegenerative disorders), pulmonary diseases, cancer, inflammatory disorders, infectious diseases, sepsis, wound healing, regenerative medicine. A common thread in the Medical Research Merit Programs is the use of cell, organ culture systems and mouse/human tissues that benefit from imaging-based analyses. In this proposal we aim to obtain funds to purchase the laser based, confocal equipped CELLINSIGHT CX7 High Content Screening Platform in order to meet the growing needs for more advanced and versatile imaging tools by the scientific community at the VA Pittsburgh. The funded research programs of these investigators require many capabilities: fixed imaging of a multitude of targets simultaneously, high-resolution discrimination of subcellular localization, cultured cells, slice tissue cultures and live cell imaging in combination with robust, reliable and versatile quantitative analyses. We identified the laser based, confocal equipped CELLINSIGHT CX7 High Content Screening Platform as a single instrument that provides the multiplexing capability using tissues, cells, organoids and intelligent algorithms for quantitative analyses. This instrument is not available either at the VA Pittsburgh or at our affiliate, the University of Pittsburgh. The acquisition of this equipment is timely with the recent opening of the VA’s state-of-the-art research facility where the equipment will be housed. A rational administrative plan has been developed to effectively manage efficient use of the platform and provide oversight for maintenance. The overall impact of the acquisition of this equipment is broad considering that 13 established investigators will be regular users of the microscope and additional PIs are expected to become users in the future as part of a growing cadre of newly VA funded scientists. By enabling VA investigators to obtain high quality data, we anticipate a seamless exchange of scientific information and an enhanced opportunity for collaborative projects between VA investigators and the University of Pittsburgh. Furthermore, two of the co-investigators are CDA awardees. The mentored stage of a clinician scientist is most critical for the successful development of their academic career. This new HCS platform will enhance their productivity by offering greater imaging quality and speed of data acquisition and help them transition from mentored to independent investigator status. Finally, the availability of this platform in the VA laboratories is expected to create opportunities for collaborations and synergize projects that have complementary focus.

匹兹堡退伍军人管理局的研究涵盖广泛的医学领域，旨在 改善退伍军人中普遍存在的各种疾病的诊断和治疗 与一般人群一样，这代表了 ORD 资金和战略规划的优先事项。主要领域 VA 的研究涵盖神经系统疾病（中风、创伤性脑损伤、神经退行性 疾病）、肺部疾病、癌症、炎症性疾病、传染病、败血症、伤口愈合、 再生医学。医学研究优异计划的一个共同点是使用细胞、器官 受益于基于成像的分析的培养系统和小鼠/人体组织。 在此提案中，我们的目标是获得资金来购买基于激光、配备共焦的 CELLINSIGHT CX7高内涵筛选平台，以满足日益增长的更先进和多功能的需求 匹兹堡退伍军人管理局科学界的成像工具。这些资助的研究项目 研究人员需要多种能力：同时对多个目标进行固定成像、高分辨率 亚细胞定位、培养细胞、切片组织培养和活细胞成像的区分 与稳健、可靠和多功能的定量分析相结合。我们确定了基于激光的共焦 配备 CELLINSIGHT CX7 高内涵筛选平台作为单一仪器，提供 使用组织、细胞、类器官和智能算法进行定量分析的多重功能。这 VA 匹兹堡大学或我们的附属机构匹兹堡大学均不提供该仪器。 该设备的采购恰逢 VA 最先进的研究最近开放 存放设备的设施。制定了合理的行政计划，以有效地 管理平台的有效使用并提供维护监督。总体影响 考虑到 13 名常设调查员将是该设备的经常用户，采购该设备的范围很广。 显微镜和其他 PI 预计将在未来成为用户，作为不断壮大的新的骨干队伍的一部分。 VA 资助科学家。通过使 VA 调查人员能够获得高质量的数据，我们预计可以实现无缝的 交流科学信息并增加 VA 之间合作项目的机会 研究人员和匹兹堡大学。此外，其中两名联合研究者是 CDA 获奖者。这 临床科学家的指导阶段对于其学术生涯的成功发展至关重要。 这个新的 HCS 平台将通过提供更高的成像质量和数据速度来提高他们的生产力 获取并帮助他们从受指导的状态过渡到独立研究者的状态。最后，可用性 VA 实验室的这个平台预计将为合作和项目协同创造机会 具有互补的重点。