Downstream sample analyses from 3 NHP species infected with SARS-CoV-2.

对感染 SARS-CoV-2 的 3 个 NHP 物种进行下游样本分析。

• 批准号: 10177667
• 负责人: JOANNE TURNER
• 金额: $ 117.93万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-15 至 2022-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177667
• 关键词: 2019-nCoV; Acute; Address; Adult; Adult Respiratory Distress Syndrome; Age; Animal Model; Animals; Antibodies; B-Lymphocytes; Brain; COVID-19; Callithrix; Callithrix jacchus jacchus; Caregivers; Cells; Centers for Disease Control and Prevention (U.S.); Cessation of life; Clinical; Communicable Diseases; Coronavirus; Development; Drug resistance; Early identification; Elderly; Environment; Evolution; Formalin; Goals; Healthcare; Heart; Immune; Immune response; Immunity; Immunocompromised Host; In Vitro; Individual; Infection; Inflammation; Intestines; Knowledge; Lead; Link; Liquid substance; Lung; Macaca; Macaca mulatta; Maintenance; Mutation; Nursing Homes; Organ; Outcome; Papio; Pathogenicity; Pathology; Peripheral; Phase; Phenotype; Proteins; Proteomics; Recovery; Reporting; Resistance; Sampling; Symptoms; T-Lymphocyte; Testing; Texas; Therapeutic; Tissues; Vaccine Design; Vaccines; Viral; Viremia; Virus; Vulnerable Populations; adaptive immune response; age group; antigen-specific T cells; associated symptom; coronavirus disease; cytokine release syndrome; experimental study; juvenile animal; lymph nodes; oxidation; pandemic disease; pressure; response; sample collection; surfactant; transmission process; vaccine development; virus development

Over the last three months, COVID-19 has emerged as a major pandemic. Coronavirus SARS-CoV-2, the causative agent of COVID-19, has remarkable infectiousness and pathogenicity, particularly in the elderly and people with immunocompromising conditions.1 The CDC have reported that 8 out of 10 deaths reported in the US related to COVID-19 are in adults 65 years and older. Similar to many other pulmonary infectious diseases, the elderly can present with atypical symptoms and initial signs of SARS-CoV-2 infection may be missed resulting in ongoing infection transmission. Texas Biomed recently infected two different age groups of common marmoset, rhesus macaque and baboon with SARS-CoV-2. Each species had differing infection outcomes. Extensive sample collection occurred throughout the 14-day study, providing an array of banked samples from 3 species of NHP, two age groups, and infected and non infected controls. We propose five Aims using banked samples, to increase our understanding of SARS-CoV-2 infection and the host response in old age. Aim 1 will investigate whether the pulmonary environment during SARS-CoV-2 infection results in oxidation of host innate molecules and a possible link to COVID-19 Acute Respiratory Distress Syndrome (CARDS). Aim 2 will determine the phenotype and function of resident and infiltrating innate immune cells during SARS- CoV-2 infection with a focus on the initiation and maintenance of a cytokine storm. Aim 3 will dissect the T and B cell response in lung and lymph nodes during early SARS-CoV-2 infection that may lead to relevant information about long term protective immunity. Aim 4 will focus on the virus during infection, determining whether viral mutations occur and whether they differ between species and age. Aim 5 will support in- depth analyses of formalin fixed tissues with a goal of viewing SARS-CoV-2 infection beyond the lung and identifying correlates of infection progression in organs such as the brain, heart and intestine.

在过去三个月里，COVID-19已成为一场重大流行病。冠状病毒SARS-CoV-2

项目成果

Interaction between type 2 diabetes and past COVID-19 on active tuberculosis.

2 型糖尿病和过去的 COVID-19 对活动性结核病的相互作用。

• DOI: 10.21203/rs.3.rs-3989104/v1
• 发表时间: 2024
• 期刊: Research square
• 作者: Calles-Cabanillas,LizE;Aguillón-Durán,GenesisP;Ayala,Doris;Caso,JoséA;Garza,Miguel;Joya-Ayala,Mateo;Cruz-Gonzalez,AmericaM;Loera-Salazar,Raul;Prieto-Martinez,Ericka;Rodríguez-Herrera,JavierE;Garcia-Oropesa,EsperanzaM;Thomas,Jo
• 通讯作者: Thomas,Jo

Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people.

• DOI: 10.1016/j.ijid.2021.08.014
• 发表时间: 2021-10
• 期刊: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
• 作者: Scordo JM;Aguillón-Durán GP;Ayala D;Quirino-Cerrillo AP;Rodríguez-Reyna E;Joya-Ayala M;Mora-Guzmán F;Ledezma-Campos E;Villafañez A;Schlesinger LS;Torrelles JB;Turner J;Restrepo BI
• 通讯作者: Restrepo BI

A prospective cross-sectional study of tuberculosis in elderly Hispanics reveals that BCG vaccination at birth is protective whereas diabetes is not a risk factor.

• DOI: 10.1371/journal.pone.0255194
• 发表时间: 2021
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Scordo JM;Aguillón-Durán GP;Ayala D;Quirino-Cerrillo AP;Rodríguez-Reyna E;Mora-Guzmán F;Caso JA;Ledezma-Campos E;Schlesinger LS;Torrelles JB;Turner J;Restrepo BI
• 通讯作者: Restrepo BI

The Aging Human Lung Mucosa: A Proteomics Study.

衰老的人类肺粘膜：蛋白质组学研究。

• DOI: 10.1093/gerona/glac091
• 发表时间: 2022
• 期刊: The journals of gerontology. Series A, Biological sciences and medical sciences
• 作者: Garcia-Vilanova,Andreu;Olmo-Fontánez,AngélicaM;Moliva,JuanI;Allué-Guardia,Anna;Singh,Harjinder;Merritt,RobertE;Maselli,DiegoJ;Peters,JayI;Restrepo,BlancaI;Wang,Yufeng;Schlesinger,LarryS;Turner,Joanne;Weintraub,SusanT;Torr
• 通讯作者: Torr

Tuberculosis presentation and outcomes in older Hispanic adults from Tamaulipas, Mexico.

• DOI: 10.1097/md.0000000000035458
• 发表时间: 2023-10-13
• 期刊: Medicine
• 影响因子: 1.6