CytoFLEX LX Cell Analyzer

总结 UCSC 提议购买一台 Beckmann and Coulter CytoFLEX LX 细胞分析仪，配有 6 个激光器（紫外、紫光、 蓝色、黄绿色、红色、远红色）和一个装板机配件。我们当前的老化 3 激光 LSR II 流式细胞仪 必须更换，因为该型号的维护将于 2021 年停止。此外，大多数用户 需要现代细胞分析仪，为细胞分析提供更多颜色，以及小样本量和 上板机提供高通量。 CytoFLEX LX 细胞分析仪将允许用户询问 单个细胞同时被多达 20 种不同的荧光团标记。印版装载机将简化 分析多个样品，并显着减少所需的样品体积，从 4 mL 样品管减少到 96 孔板中的孔。小样本量对于我们许多干细胞研究人员来说尤其重要 他们经常使用非常稀有的原代细胞。 主要用户将依靠这种新仪器来推进 NIH 资助的重要生物医学问题 研究资助，包括以下方面的调查：a) 年龄引起的造血干细胞变化及其影响 老年疾病，b) 一种新型的、发育受限的造血干细胞的作用和调节，c) 长链非编码 RNA GAPLINC 在感染性休克和炎症中的作用，以及 d) 机制 控制乳腺细胞命运的获得和肺泡发生。小用户将解决不同的问题 生物医学问题，例如建立细菌病原体感染的细节、干细胞的调节 人类发育中的细胞分化、药物和肽的细胞膜渗透性以及 用于药物开发的天然产物的功能表征。 拟议的仪器将安装在 UCSC 的细胞计数核心设施中，该设施于 2007.使用仪器的仪器监督、培训、维护调度和充值核算 拟议的仪器将由我们的细胞计数核心设施经理保证，其工资由 UCSC。

Summary UCSC proposes to purchase a Beckmann and Coulter CytoFLEX LX cell analyzer with 6 lasers (UV, violet, blue, yellow-green, red, far red) and a plate-loader accessory. Our current, aging, 3-laser LSR II flow cytometer must be replaced, because maintenance for that model will be discontinued in 2021. Additionally, most users require a modern cell analyzer that offers more colors for cell analysis, as well as the small sample volume and high throughput afforded by the plate-loader. The CytoFLEX LX cell analyzer will allow users to interrogate individual cells that are labeled with up to 20 different fluorophores at once. The plate loader will streamline the analysis of multiple samples and reduce the required sample volume significantly from 4-mL sample tubes to wells in a 96-well plate. The small sample volume is particularly important for our many stem cell researchers who often work with very rare, primary cells. Major users will rely on this new instrument to advance important biomedical issues in their NIH-funded research grants, including investigations of: a) age-induced changes in blood stem cells and implications for geriatric ailments, b) the role and regulation of a novel, developmentally restricted hematopoietic stem cell, c) the role of the long non-coding RNA GAPLINC in septic shock and inflammation, and d) the mechanisms governing cell fate acquisition and alveologenesis in the mammary gland. Minor users will address diverse biomedical problems, such as establishing the details of bacterial pathogen infections, the regulation of stem cell differentiation in human development, cell membrane permeability for drugs, and peptides, and the functional characterization of natural products for drug development. The proposed instrument will be housed in UCSC’s Cytometry Core facility, which was established in 2007. Instrument supervision, training, maintenance scheduling, and recharge accounting for use of the proposed instrument will be assured by our Cytometry Core Facility manager, whose salary is guaranteed by the UCSC.