CytoFLEX LX Cell Analyzer

摘要 UCSC建议购买Beckmann and Coulter CytoFLEX LX细胞分析仪，配备6种激光(紫外线、紫外光、 蓝色、黄绿色、红色、远红色)和一个板式装载机配件。我们目前老化的3-激光LSR II流式细胞仪 必须更换，因为该型号的维护将于2021年停止。此外，大多数用户 需要一种现代细胞分析仪，为细胞分析提供更多的颜色，以及较小的样本量和 由板式装载机提供的高吞吐量。CytoFLEX LX细胞分析仪将允许用户询问 单个细胞一次被标记多达20个不同的荧光团。板材装载机将简化 分析多个样品，并将所需的样品量从4毫升样品管大幅减少到 韦尔斯在一个96井的盘子里。小样本量对我们许多干细胞研究人员来说特别重要 他们经常与非常稀有的原代细胞一起工作。 主要用户将依靠这一新工具来推动他们由NIH资助的重要生物医学问题 研究补助金，包括调查：a)年龄引起的血液干细胞变化及其对 老年病，b)一种新的、发育受限的造血干细胞的作用和调节，c) 长非编码RNA GAPLINC在感染性休克和炎症中的作用，以及d)机制 控制乳腺细胞命运的获得和肺泡形成。较小的用户将满足多样化的需求 生物医学问题，如确定细菌病原体感染的细节，茎的调节 人类发育中的细胞分化，药物和多肽的细胞膜通透性，以及 用于药物开发的天然产物的功能表征。 拟议中的仪器将安装在加州大学伯克利分校的细胞学核心设施中，该设施成立于 2007年。使用的仪器监督、培训、维护计划和充值会计 建议的仪器将由我们的细胞学核心设施经理保证，他的工资由 加州大学洛杉矶分校。

Summary UCSC proposes to purchase a Beckmann and Coulter CytoFLEX LX cell analyzer with 6 lasers (UV, violet, blue, yellow-green, red, far red) and a plate-loader accessory. Our current, aging, 3-laser LSR II flow cytometer must be replaced, because maintenance for that model will be discontinued in 2021. Additionally, most users require a modern cell analyzer that offers more colors for cell analysis, as well as the small sample volume and high throughput afforded by the plate-loader. The CytoFLEX LX cell analyzer will allow users to interrogate individual cells that are labeled with up to 20 different fluorophores at once. The plate loader will streamline the analysis of multiple samples and reduce the required sample volume significantly from 4-mL sample tubes to wells in a 96-well plate. The small sample volume is particularly important for our many stem cell researchers who often work with very rare, primary cells. Major users will rely on this new instrument to advance important biomedical issues in their NIH-funded research grants, including investigations of: a) age-induced changes in blood stem cells and implications for geriatric ailments, b) the role and regulation of a novel, developmentally restricted hematopoietic stem cell, c) the role of the long non-coding RNA GAPLINC in septic shock and inflammation, and d) the mechanisms governing cell fate acquisition and alveologenesis in the mammary gland. Minor users will address diverse biomedical problems, such as establishing the details of bacterial pathogen infections, the regulation of stem cell differentiation in human development, cell membrane permeability for drugs, and peptides, and the functional characterization of natural products for drug development. The proposed instrument will be housed in UCSC’s Cytometry Core facility, which was established in 2007. Instrument supervision, training, maintenance scheduling, and recharge accounting for use of the proposed instrument will be assured by our Cytometry Core Facility manager, whose salary is guaranteed by the UCSC.