CytoFLEX LX Cell Analyzer
CytoFLEX LX Cell Analyzer
批准号:
10177735
负责人:
CAMILLA FORSBERG
金额:
$29.68万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-09-20 至 2022-09-19
关键词:
AccountingAddressAgeAgingCell Membrane PermeabilityCellsColorCore FacilityCytometryDevelopmentElderlyFundingHematopoietic stem cellsHuman DevelopmentIndividualInfectionInflammationInvestigationLabelLasersMaintenanceMammary glandMinorModelingModernizationNatural Product DrugPeptidesPharmaceutical PreparationsRegulationResearch PersonnelResearch Project GrantsRoleSamplingScheduleSeptic ShockSupervisionTrainingTubeUnited States National Institutes of HealthUntranslated RNAViolaWagesWorkcell analyzerdrug developmentfluorophoreinstrumentnovelpathogenic bacteriastem cell differentiationstem cells
中文摘要
总结
UCSC 提议购买一台 Beckmann and Coulter CytoFLEX LX 细胞分析仪,配有 6 个激光器(紫外、紫光、
蓝色、黄绿色、红色、远红色)和一个装板机配件。我们当前的老化 3 激光 LSR II 流式细胞仪
必须更换,因为该型号的维护将于 2021 年停止。此外,大多数用户
需要现代细胞分析仪,为细胞分析提供更多颜色,以及小样本量和
上板机提供高通量。 CytoFLEX LX 细胞分析仪将允许用户询问
单个细胞同时被多达 20 种不同的荧光团标记。印版装载机将简化
分析多个样品,并显着减少所需的样品体积,从 4 mL 样品管减少到
96 孔板中的孔。小样本量对于我们许多干细胞研究人员来说尤其重要
他们经常使用非常稀有的原代细胞。
主要用户将依靠这种新仪器来推进 NIH 资助的重要生物医学问题
研究资助,包括以下方面的调查:a) 年龄引起的造血干细胞变化及其影响
老年疾病,b) 一种新型的、发育受限的造血干细胞的作用和调节,c)
长链非编码 RNA GAPLINC 在感染性休克和炎症中的作用,以及 d) 机制
控制乳腺细胞命运的获得和肺泡发生。小用户将解决不同的问题
生物医学问题,例如建立细菌病原体感染的细节、干细胞的调节
人类发育中的细胞分化、药物和肽的细胞膜渗透性以及
用于药物开发的天然产物的功能表征。
拟议的仪器将安装在 UCSC 的细胞计数核心设施中,该设施于
2007.使用仪器的仪器监督、培训、维护调度和充值核算
拟议的仪器将由我们的细胞计数核心设施经理保证,其工资由
UCSC。
英文摘要
Summary
UCSC proposes to purchase a Beckmann and Coulter CytoFLEX LX cell analyzer with 6 lasers (UV, violet,
blue, yellow-green, red, far red) and a plate-loader accessory. Our current, aging, 3-laser LSR II flow cytometer
must be replaced, because maintenance for that model will be discontinued in 2021. Additionally, most users
require a modern cell analyzer that offers more colors for cell analysis, as well as the small sample volume and
high throughput afforded by the plate-loader. The CytoFLEX LX cell analyzer will allow users to interrogate
individual cells that are labeled with up to 20 different fluorophores at once. The plate loader will streamline the
analysis of multiple samples and reduce the required sample volume significantly from 4-mL sample tubes to
wells in a 96-well plate. The small sample volume is particularly important for our many stem cell researchers
who often work with very rare, primary cells.
Major users will rely on this new instrument to advance important biomedical issues in their NIH-funded
research grants, including investigations of: a) age-induced changes in blood stem cells and implications for
geriatric ailments, b) the role and regulation of a novel, developmentally restricted hematopoietic stem cell, c)
the role of the long non-coding RNA GAPLINC in septic shock and inflammation, and d) the mechanisms
governing cell fate acquisition and alveologenesis in the mammary gland. Minor users will address diverse
biomedical problems, such as establishing the details of bacterial pathogen infections, the regulation of stem
cell differentiation in human development, cell membrane permeability for drugs, and peptides, and the
functional characterization of natural products for drug development.
The proposed instrument will be housed in UCSC’s Cytometry Core facility, which was established in
2007. Instrument supervision, training, maintenance scheduling, and recharge accounting for use of the
proposed instrument will be assured by our Cytometry Core Facility manager, whose salary is guaranteed by
the UCSC.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
A postdoctoral training program for impactful careers in stem cell biology
-
批准号:10410891
-
项目类别:
-
资助金额:$14.95万
-
财政年份:2022
-
负责人:CAMILLA FORSBERG
-
依托单位:
IRACDA at UCSC and CSUMB
-
批准号:10676248
-
项目类别:
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资助金额:$97.92万
-
财政年份:2020
-
负责人:CAMILLA FORSBERG
-
依托单位:
IRACDA at UCSC and CSUMB
-
批准号:10460280
-
项目类别:
-
资助金额:$97.82万
-
财政年份:2020
-
负责人:CAMILLA FORSBERG
-
依托单位:
IRACDA at UCSC and CSUMB
-
批准号:10056092
-
项目类别:
-
资助金额:$44.71万
-
财政年份:2020
-
负责人:CAMILLA FORSBERG
-
依托单位:
IRACDA at UCSC and CSUMB
-
批准号:10239236
-
项目类别:
-
资助金额:$72.06万
-
财政年份:2020
-
负责人:CAMILLA FORSBERG
-
依托单位:
Defining the cause and consequence of aging-specific platelet specification from hematopoietic stem cells
-
批准号:10399429
-
项目类别:
-
资助金额:$54.69万
-
财政年份:2019
-
负责人:CAMILLA FORSBERG
-
依托单位:
Defining the cause and consequence of aging-specific platelet specification from hematopoietic stem cells
-
批准号:10621901
-
项目类别:
-
资助金额:$54.69万
-
财政年份:2019
-
负责人:CAMILLA FORSBERG
-
依托单位:
Defining the cause and consequence of aging-specific platelet specification from hematopoietic stem cells
-
批准号:10100559
-
项目类别:
-
资助金额:$13.49万
-
财政年份:2019
-
负责人:CAMILLA FORSBERG
-
依托单位:
Defining the cause and consequence of aging-specific platelet specification from hematopoietic stem cells
-
批准号:10017134
-
项目类别:
-
资助金额:$55.15万
-
财政年份:2019
-
负责人:CAMILLA FORSBERG
-
依托单位:
Role and Regulation of a Novel, Developmentally Restricted Hematopoietic Stem Cell
-
批准号:10407592
-
项目类别:
-
资助金额:$45.53万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
Role and Regulation of a Novel, Developmentally Restricted Hematopoietic Stem Cel
-
批准号:8614497
-
项目类别:
-
资助金额:$32.78万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
Regulation of Chromatin Structure Dynamics in Hematopoietic and Leukemic Stem Cel
-
批准号:8706954
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项目类别:
-
资助金额:$40.79万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
Role and Regulation of a Novel, Developmentally Restricted Hematopoietic Stem Cel
-
批准号:9308600
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项目类别:
-
资助金额:$0.34万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
Regulation of Chromatin Structure Dynamics in Hematopoietic and Leukemic Stem Cel
-
批准号:9100848
-
项目类别:
-
资助金额:$44.22万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
Role and Regulation of a Novel, Developmentally Restricted Hematopoietic Stem Cell
-
批准号:10224171
-
项目类别:
-
资助金额:$45.53万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
Regulation of Chromatin Structure Dynamics in Hematopoietic and Leukemic Stem Cel
-
批准号:8578535
-
项目类别:
-
资助金额:$37.77万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
In vivo mouse models for inducible hematopoietic- and hematopoietic stem cell-spe
-
批准号:8583792
-
项目类别:
-
资助金额:$20.64万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
Role and Regulation of a Novel, Developmentally Restricted Hematopoietic Stem Cel
-
批准号:8733686
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项目类别:
-
资助金额:$32.88万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
In vivo mouse models for inducible hematopoietic- and hematopoietic stem cell-spe
-
批准号:8719927
-
项目类别:
-
资助金额:$18.11万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
Role and Regulation of a Novel, Developmentally Restricted Hematopoietic Stem Cel
-
批准号:9132252
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项目类别:
-
资助金额:$33.09万
-
财政年份:2013
-
负责人:CAMILLA FORSBERG
-
依托单位:
海外基金