CytoFLEX LX Cell Analyzer

总结 UCSC建议购买Beckmann和Coulter CytoFLEX LX细胞分析仪，配有6个激光器（UV，紫光， 蓝色、黄绿色、红色、远红色）和印版装载器附件。我们目前的老化3激光LSR II流式细胞仪 必须更换，因为该型号的维护将于2021年停止。此外，大多数用户 需要一个现代化的细胞分析仪，为细胞分析提供更多的颜色，以及小的样品体积， 板装载机提供的高通量。CytoFLEX LX细胞分析仪将允许用户询问 单个细胞同时被多达20种不同的荧光团标记。板装载机将简化 分析多个样品，并将所需的样品体积从4 mL样品管显著减少到 96孔板中的威尔斯孔。小样本量对于我们众多的干细胞研究者来说尤为重要 who often经常work with very rare罕见，primary原代cells细胞. 主要用户将依靠这一新工具来推进其NIH资助的重要生物医学问题。 研究赠款，包括调查：a）年龄引起的造血干细胞变化及其对 老年病，B）一种新的发育受限的造血干细胞的作用和调节，c） 长链非编码RNA GAPLINC在感染性休克和炎症中的作用，以及d）其机制 控制乳腺中的细胞命运获得和肺泡发生。少数用户将解决各种 生物医学问题，如建立细菌病原体感染的细节，干细胞的调节， 人类发育中的细胞分化，药物和肽的细胞膜渗透性，以及 用于药物开发的天然产物的功能表征。 拟议的仪器将被安置在UCSC的细胞计数核心设施，这是建立在 2007.仪器监督、培训、维护计划和使用的再充电核算 建议的仪器将由我们的细胞计数核心设施经理保证，其工资由 UCSC。

Summary UCSC proposes to purchase a Beckmann and Coulter CytoFLEX LX cell analyzer with 6 lasers (UV, violet, blue, yellow-green, red, far red) and a plate-loader accessory. Our current, aging, 3-laser LSR II flow cytometer must be replaced, because maintenance for that model will be discontinued in 2021. Additionally, most users require a modern cell analyzer that offers more colors for cell analysis, as well as the small sample volume and high throughput afforded by the plate-loader. The CytoFLEX LX cell analyzer will allow users to interrogate individual cells that are labeled with up to 20 different fluorophores at once. The plate loader will streamline the analysis of multiple samples and reduce the required sample volume significantly from 4-mL sample tubes to wells in a 96-well plate. The small sample volume is particularly important for our many stem cell researchers who often work with very rare, primary cells. Major users will rely on this new instrument to advance important biomedical issues in their NIH-funded research grants, including investigations of: a) age-induced changes in blood stem cells and implications for geriatric ailments, b) the role and regulation of a novel, developmentally restricted hematopoietic stem cell, c) the role of the long non-coding RNA GAPLINC in septic shock and inflammation, and d) the mechanisms governing cell fate acquisition and alveologenesis in the mammary gland. Minor users will address diverse biomedical problems, such as establishing the details of bacterial pathogen infections, the regulation of stem cell differentiation in human development, cell membrane permeability for drugs, and peptides, and the functional characterization of natural products for drug development. The proposed instrument will be housed in UCSC’s Cytometry Core facility, which was established in 2007. Instrument supervision, training, maintenance scheduling, and recharge accounting for use of the proposed instrument will be assured by our Cytometry Core Facility manager, whose salary is guaranteed by the UCSC.