CytoFLEX LX Cell Analyzer

CytoFLEX LX 细胞分析仪

• 批准号: 10177735
• 负责人: CAMILLA FORSBERG
• 金额: $ 29.68万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-20 至 2022-09-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177735
• 关键词: Accounting; Address; Age; Aging; Cell Membrane Permeability; Cells; Color; Core Facility; Cytometry; Development; Elderly; Funding; Hematopoietic stem cells; Human Development; Individual; Infection; Inflammation; Investigation; Label; Lasers; Maintenance; Mammary gland; Minor; Modeling; Modernization; Natural Product Drug; Peptides; Pharmaceutical Preparations; Regulation; Research Personnel; Research Project Grants; Role; Sampling; Schedule; Septic Shock; Supervision; Training; Tube; United States National Institutes of Health; Untranslated RNA; Viola; Wages; Work; cell analyzer; drug development; fluorophore; instrument; novel; pathogenic bacteria; stem cell differentiation; stem cells

Summary UCSC proposes to purchase a Beckmann and Coulter CytoFLEX LX cell analyzer with 6 lasers (UV, violet, blue, yellow-green, red, far red) and a plate-loader accessory. Our current, aging, 3-laser LSR II flow cytometer must be replaced, because maintenance for that model will be discontinued in 2021. Additionally, most users require a modern cell analyzer that offers more colors for cell analysis, as well as the small sample volume and high throughput afforded by the plate-loader. The CytoFLEX LX cell analyzer will allow users to interrogate individual cells that are labeled with up to 20 different fluorophores at once. The plate loader will streamline the analysis of multiple samples and reduce the required sample volume significantly from 4-mL sample tubes to wells in a 96-well plate. The small sample volume is particularly important for our many stem cell researchers who often work with very rare, primary cells. Major users will rely on this new instrument to advance important biomedical issues in their NIH-funded research grants, including investigations of: a) age-induced changes in blood stem cells and implications for geriatric ailments, b) the role and regulation of a novel, developmentally restricted hematopoietic stem cell, c) the role of the long non-coding RNA GAPLINC in septic shock and inflammation, and d) the mechanisms governing cell fate acquisition and alveologenesis in the mammary gland. Minor users will address diverse biomedical problems, such as establishing the details of bacterial pathogen infections, the regulation of stem cell differentiation in human development, cell membrane permeability for drugs, and peptides, and the functional characterization of natural products for drug development. The proposed instrument will be housed in UCSC’s Cytometry Core facility, which was established in 2007. Instrument supervision, training, maintenance scheduling, and recharge accounting for use of the proposed instrument will be assured by our Cytometry Core Facility manager, whose salary is guaranteed by the UCSC.

概括 UCSC提议购买贝克曼和Coulter Cytoflex LX Cell分析仪，该分析仪具有6个激光器（UV，Violet， 蓝色，黄绿色，红色，红色）和板载器附件。我们当前的，老化的3射线LSR II流式细胞仪 必须更换，因为该模型的维护将在2021年停止。此外，大多数用户 需要一个现代的细胞分析仪，该分析仪提供更多用于细胞分析的颜色，以及较小的样品体积和 板载器提供的高吞吐量。 CytoFlex LX单元分析仪将允许用户询问 单个细胞一次标记多达20种不同的荧光团。板装载机将简化 分析多个样品，并将所需的样品体积从4 ml样品管显着减少到 在96孔板中的井。小样本体积对我们的许多干细胞研究人员尤为重要 他们经常与非常罕见的原代细胞一起工作。 主要用户将依靠这种新工具来推进其NIH资助的重要生物医学问题 研究补助金，包括：a）年龄引起的血液干细胞变化及其对 老年疾病，b）新型，受限制的造血干细胞的作用和调节，c） 长的非编码RNA GAPLINC在败血性休克和注射中的作用，d）机制 乳腺中治理细胞脂肪获取和肺泡术。次要用户将解决多样化 生物医学问题，例如建立细菌病原体感染的细节，调节茎 人类发育中的细胞分化，药物的细胞膜通透性，肽以及 天然产物用于药物开发的功能表征。 该拟议的仪器将安置在UCSC的细胞仪核心设施中，该设施建立在 2007年。仪器监督，培训，维护计划和补给 建议的工具将由我们的细胞仪核心设施经理承担，其工资由 UCSC。