Cryo Transmission Electron Microscope for Cryo-EM Sample Optimization

用于冷冻电镜样品优化的冷冻透射电子显微镜

• 批准号: 10177173
• 负责人: Elizabeth Kellogg
• 金额: $ 105.14万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177173
• 关键词: Biological; Communities; Complex; Cryoelectron Microscopy; Crystallization; Data Collection; Electron Microscope; Faculty; Freezing; Funding; Future; Genome; Health; Human; Image; Membrane; Micro Electron Diffraction; Microscope; Negative Staining; Nucleic Acids; Request for Applications; Research; Research Personnel; Resolution; Sampling; Schedule; Scientist; Structure; United States National Institutes of Health; Viral; biological systems; detector; improved; programs; receptor; screening; structural biology; tool; trafficking; transmission process

PROJECT SUMMARY/ABSTRACT Cryo-electron microscopy (Cryo-EM) has heralded a resolution revolution in structural biology that has made it possible for high-resolution structures to be determined for targets that have remained recalcitrant to crystallization for decades. Cornell has invested in this future with the purchase of a high-end electron microscope, the Arctica with a K3, allowing researchers an important valuable tool with which to reveal the atomic details of their cryo-frozen biological samples. Unfortunately, we are scheduled to lose our dedicated low-end screening microscope, the Morgagni, sometime in 2021 or possibly 2022. In analyzing our current workflow and needs of the structural biology community at Cornell, we have concluded that the optimal replacement for the Morgagni is a F200Ci microscope capable of both negative stain data collection and cryo-EM imaging. Acquiring such a microscope will improve overall throughput for Cornell scientists and the NIH-funded investigators’ research programs, maximizing the utility of our highest-resolution and most in-demand microscopes which are equipped with accessories crucial for high-resolution data collection: a the K3 direct detector and a BioQuantum Energy Filter. This application requests funds to purchase a versatile cryo-electron microscope ideally capable of negative stain imaging, cryo-sample screening, and micro-electron diffraction (microED). It will serve NIH-funded faculty in five departments across Cornell, all invested in pursing the structure of biomedically relevant and important biological systems related to human health, including membrane receptors, membrane trafficking, nucleic-acid complexes involved in genome organization, and viral structure.

项目总结/摘要 冷冻电子显微镜（Cryo-EM）预示着结构生物学的分辨率革命， 有可能为仍然不确定的目标确定高分辨率结构， 几十年的结晶。康奈尔大学购买了一台高端电子设备， 显微镜，Arctica与K3，让研究人员一个重要的有价值的工具，以揭示原子 他们冷冻生物样本的详细信息不幸的是，我们将失去我们的专用低端 在2021年或2022年的某个时候，在分析我们目前的工作流程和 根据康奈尔大学结构生物学社区的需求，我们得出结论， Morgagni是一款F200 Ci显微镜，能够进行负染色数据收集和冷冻EM成像。 获得这样的显微镜将提高康奈尔大学科学家和NIH资助的 研究人员的研究计划，最大限度地利用我们的最高分辨率和最受欢迎的 显微镜配备了高分辨率数据收集的关键配件：K3直接 探测器和生物量子能量过滤器本申请要求资金购买一个多功能的低温电子 显微镜理想的负染色成像，冷冻样品筛选，微电子衍射能力 （microED）。它将为康奈尔大学五个部门的NIH资助的教师提供服务，所有部门都投资于追求结构 与人类健康有关的生物医学相关和重要的生物系统，包括膜 受体、膜运输、参与基因组组织的核酸复合物和病毒结构。

项目成果

Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavH.

• DOI: 10.1371/journal.ppat.1011512
• 发表时间: 2023-07
• 期刊: PLOS PATHOGENS
• 影响因子: 6.7
• 作者: Zhang, Qing;Wan, Min;Kudryashova, Elena;Kudryashov, Dmitri;Mao, Yuxin
• 通讯作者: Mao, Yuxin

Structures of the holo CRISPR RNA-guided transposon integration complex.

• DOI: 10.1038/s41586-022-05573-5
• 发表时间: 2023-01
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Park, Jung-Un;Tsai, Amy Wei-Lun;Rizo, Alexandrea N. N.;Truong, Vinh H. H.;Wellner, Tristan X. X.;Schargel, Richard D. D.;Kellogg, Elizabeth H. H.
• 通讯作者: Kellogg, Elizabeth H. H.