Quadrupole Orbitrap Hybrid Mass Spectrometer for Proteomics

用于蛋白质组学的四极 Orbitrap 混合质谱仪

• 批准号: 10177354
• 负责人: Lauren Elizabeth Ball
• 金额: $ 77.27万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177354
• 关键词: Address; Award; Cardiovascular Diseases; Diabetes Mellitus; Digestive System Disorders; Disease; Funding; Hormones; Hybrids; Immunotherapy; Institution; Malignant Neoplasms; Mass Spectrum Analysis; Medical; Musculoskeletal Diseases; Mutation; Pharmaceutical Preparations; Post-Translational Protein Processing; Proteins; Proteomics; Regulation; Research; Research Personnel; Resources; Sampling; South Carolina; System; Time; United States National Institutes of Health; Universities; addiction; experience; experimental study; instrument; instrumentation; mass spectrometer; nervous system disorder; protein expression; success

PROJECT SUMMARY This proposal is requesting funds to acquire a Quadrupole Orbitrap Hybrid Exploris 480 Mass Spectrometer (ThermoScientific) for the Medical University of South Carolina Mass Spectrometry Facility and Proteomics Center to support our NIH funded investigators’ efforts to discover mechanisms of protein regulation involved in cardiovascular disease, cancer, immunotherapy, digestive disease, diabetes, musculoskeletal diseases, addiction, and neurological diseases. Our users are relying on ThermoScientific Orbitrap LC-MS/MS ETD systems purchased in 2012 and 2018 (S10 OD010731 and S10 OD025126, PI: Lauren Ball) to characterize the impact of drugs/hormones, genetic alterations, and disease on changes in protein expression, protein interactions, and post-translational modifications. With the recent funding successes of new users and an increased number of NIH awards to our major user group, we are experiencing a greater demand for instrument time. To address the increased need for quantitative proteomic experiments and provide highly-sensitive, targeted-proteomic capabilities for the analysis of post-translationally modified proteins from limited amounts of sample, we are requesting the state-of-the-art Quadrupole Orbitrap Hybrid Exploris 480 MS. The requested instrumentation is to be housed in the Mass Spectrometry Facility, a University Research Resource Facility, to provide quantitative proteomic capabilities to investigators at the Medical University of South Carolina and other academic research institutions in South Carolina.

项目概要 该提案要求资金购买四极 Orbitrap Hybrid Exploris 480 质谱仪 (ThermoScientific) 南卡罗来纳医科大学质谱设施和蛋白质组学 该中心支持我们的 NIH 资助的研究人员发现参与的蛋白质调控机制的努力 心血管疾病、癌症、免疫治疗、消化系统疾病、糖尿病、肌肉骨骼疾病、 成瘾和神经系统疾病。我们的用户信赖 ThermoScientific Orbitrap LC-MS/MS ETD 2012 年和 2018 年购买的系统（S10 OD010731 和 S10 OD025126，PI：Lauren Ball）来表征 药物/激素、基因改变和疾病对蛋白质表达、蛋白质变化的影响 相互作用和翻译后修饰。随着新用户最近的融资成功和 我们的主要用户群体获得的 NIH 奖项数量增加，我们对仪器的需求也越来越大 时间。为了满足定量蛋白质组学实验日益增长的需求，并提供高灵敏度、 靶向蛋白质组学能力，用于分析有限数量的翻译后修饰蛋白质 对于样品，我们需要最先进的四极 Orbitrap Hybrid Exploris 480 MS。所要求的 仪器将安置在质谱设施（大学研究资源设施）中，以 为南卡罗来纳医科大学和其他大学的研究人员提供定量蛋白质组学能力 南卡罗来纳州的学术研究机构。