Quadrupole Orbitrap Hybrid Mass Spectrometer for Proteomics

用于蛋白质组学的四极 Orbitrap 混合质谱仪

• 批准号: 10177354
• 负责人: Lauren Elizabeth Ball
• 金额: $ 77.27万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177354
• 关键词: Address; Award; Cardiovascular Diseases; Diabetes Mellitus; Digestive System Disorders; Disease; Funding; Hormones; Hybrids; Immunotherapy; Institution; Malignant Neoplasms; Mass Spectrum Analysis; Medical; Musculoskeletal Diseases; Mutation; Pharmaceutical Preparations; Post-Translational Protein Processing; Proteins; Proteomics; Regulation; Research; Research Personnel; Resources; Sampling; South Carolina; System; Time; United States National Institutes of Health; Universities; addiction; experience; experimental study; instrument; instrumentation; mass spectrometer; nervous system disorder; protein expression; success

PROJECT SUMMARY This proposal is requesting funds to acquire a Quadrupole Orbitrap Hybrid Exploris 480 Mass Spectrometer (ThermoScientific) for the Medical University of South Carolina Mass Spectrometry Facility and Proteomics Center to support our NIH funded investigators’ efforts to discover mechanisms of protein regulation involved in cardiovascular disease, cancer, immunotherapy, digestive disease, diabetes, musculoskeletal diseases, addiction, and neurological diseases. Our users are relying on ThermoScientific Orbitrap LC-MS/MS ETD systems purchased in 2012 and 2018 (S10 OD010731 and S10 OD025126, PI: Lauren Ball) to characterize the impact of drugs/hormones, genetic alterations, and disease on changes in protein expression, protein interactions, and post-translational modifications. With the recent funding successes of new users and an increased number of NIH awards to our major user group, we are experiencing a greater demand for instrument time. To address the increased need for quantitative proteomic experiments and provide highly-sensitive, targeted-proteomic capabilities for the analysis of post-translationally modified proteins from limited amounts of sample, we are requesting the state-of-the-art Quadrupole Orbitrap Hybrid Exploris 480 MS. The requested instrumentation is to be housed in the Mass Spectrometry Facility, a University Research Resource Facility, to provide quantitative proteomic capabilities to investigators at the Medical University of South Carolina and other academic research institutions in South Carolina.

项目总结 这项提案要求获得资金，以购买四极轨道混合探索480质谱仪 (热科学)南卡罗来纳医科大学质谱学和蛋白质组学 中心支持我们的NIH资助的研究人员努力发现参与蛋白质调控的机制 心血管疾病、癌症、免疫治疗、消化系统疾病、糖尿病、肌肉骨骼疾病、 上瘾和神经系统疾病。我们的用户依赖于ThermoScience Orbitrap LC-MS/MS ETD 2012年和2018年购买的系统(S10 OD010731和S10 OD025126，PI：Lauren Ball) 药物/激素、基因改变和疾病对蛋白质表达、蛋白质变化的影响 相互作用和翻译后修饰。随着最近新用户的成功融资和 我们的主要用户群体获得的NIH奖项越来越多，我们对乐器的需求也越来越大 时间到了。为了满足日益增长的定量蛋白质组学实验的需求，并提供高度敏感的、 用于分析有限数量的翻译后修饰蛋白的靶向蛋白质组学能力 样品，我们请求最先进的四极轨道混合探索器480 MS。 仪器将被安置在质谱学设施中，这是一个大学研究资源设施，以 为南卡罗来纳医科大学和其他机构的研究人员提供定量蛋白质组能力 南卡罗来纳州的学术研究机构。