Proteomics Core

蛋白质组学核心

基本信息

批准号：
10005398
负责人：
Lauren Elizabeth Ball
金额：
$ 15.32万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-01 至 2022-01-15
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10005398
关键词：
Acetylation Acute Address Applications Grants Bioinformatics Biomedical Research Cells Centers of Research Excellence Complex Computer software Consultations Custom Data Data Analyses Detection Disease Enzymes Equilibrium Experimental Designs Faculty Funding Future Global Change Goals Grant Half-Life Human Resources Individual Infrastructure Institution Label Ligands Lysine Manuscripts Mass Spectrum Analysis Mediating Medical Mentors Mentorship Methodology Modification Mutation Nitrates Nutrient Oxidants Oxidation-Reduction Participant Peptides Pharmaceutical Preparations Phosphorylation Phosphorylation Site Post-Translational Modification Site Post-Translational Protein Processing Preparation Protein Chemistry Proteins Proteomics Research Research Personnel Research Project Grants Sampling Services Signal Transduction South Carolina Stress Sulfhydryl Compounds Techniques Technology Time Tissues Training Tyrosine United States National Institutes of Health Universities base career development data acquisition data archive design differential expression experience experimental study instrumentation investigator training mass spectrometer nitration phosphoproteomics protein crosslink protein expression protein function response skills success symposium

项目摘要

ABSTRACT The objective of Proteomics Core B is to provide state of the art mass spectrometry-based proteomic capabilities, dedicated analytical support, and mentorship to the investigators funded through the SC COBRE in Oxidants, Redox Balance, and Stress Signaling (Redox COBRE). Mass spectrometry is a powerful technology enabling unbiased discovery of the effects of drugs/ligands, genetic alterations, and disease on changes in protein expression, protein interactions, and post-translational modifications. Post-translational modifications encode the nutrient and stress status of the cell and mediate acute responses to ligand stimulation and changes in redox status by influencing protein function, half-life, and localization. The MUSC Mass Spectrometry Facility and Proteomics Center have an extensive array of instrumentation for protein characterization and expertise in applying proteomic technologies to biomedical research endeavors. During the last funding period acquisition of an Orbitrap Elite mass spectrometer, purchased through a shared instrumentation grant, enabled us for the first time to apply quantitative proteomic approaches to the detection of complex post-translational modifications. In ongoing collaborative projects with COBRE investigators and participants in the Redox Center, Proteomics Core B is utilizing the Orbitrap Elite to examine the global effects of altered redox status on protein expression, phosphorylation, acetylation, and thiol modifications and to characterize the sites of phosphorylation, S-glutathionylation, tyrosine nitration, and protein crosslinking on redox-sensitive target proteins. The specific aims of Proteomics Core B are: 1) to assist COBRE investigators with experimental design including sample preparation strategies and choice of specific proteomic approach, 2) to customize data acquisition and analysis to address the goals of the individual projects, and 3) to provide mentorship and training in the applications of proteomic technologies for future projects. Access to these capabilities and expertise to push the available technology forward is critical to the proposed research projects. The experimental capabilities and infrastructure established with COBRE investigators are made available to investigators at MUSC and other institutions in South Carolina that utilize the MUSC Mass Spectrometry Facility. Our goals are to provide COBRE investigators with access to instrumentation and expertise to advance their research and contribute to the success of our promising junior faculty.

抽象的蛋白质组学核心B的目的是提供基于质谱的最先进的蛋白质组学通过SC Cobre资助的调查员的能力，专用分析支持和指导在氧化剂，氧化还原平衡和应力信号传导中（氧化还原胶）。质谱是一个强大的技术可以无偏见地发现药物/配体的影响，遗传改变和疾病对蛋白质表达，蛋白质相互作用和翻译后修饰的变化。翻译后修改编码细胞的养分和应力状态并介导对配体的急性反应通过影响蛋白质功能，半衰期和定位来刺激和氧化还原状态的变化。 MUSC 质谱设施和蛋白质组学中心具有广泛的蛋白质仪器将蛋白质组学技术应用于生物医学研究的努力方面的特征和专业知识。期间 Orbitrap Elite质谱仪的最后一次融资期获得，该质谱仪是通过共享购买的仪器赠款，使我们首次将定量蛋白质组学方法应用于检测复杂的翻译后修饰。在与山羊研究人员和氧化还原中心的参与者，蛋白质组学核心B正在利用Orbitrap Elite来检查全球效果氧化还原状态在蛋白质表达，磷酸化，乙酰化和硫醇的修饰上的改变表征磷酸化，s-谷胱甘肽化，酪氨酸硝化和蛋白质交联的位点氧化还原敏感的靶蛋白。蛋白质组学核心B的具体目的是：1）协助毛cor研究者通过实验设计，包括样品制备策略和特定蛋白质组学方法的选择，2）自定义数据获取和分析以解决各个项目的目标，3）蛋白质组学技术在未来项目中应用的指导和培训。访问这些推动可用技术向前发展的能力和专业知识对于拟议的研究项目至关重要。用毛毛调查员建立的实验能力和基础设施可用于 MUSC和南卡罗来纳州其他机构的调查员利用MUSC质谱法设施。我们的目标是为工土调查人员提供仪器和专业知识的机会推进他们的研究，并为我们有前途的初级教师的成功做出贡献。