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Orbitrap Mass Spectrometer

Orbitrap 质谱仪

基本信息

批准号：
8247428
负责人：
Lauren Elizabeth Ball
金额：
$ 60万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-01 至 2013-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal is for the addition of a Thermo Scientific LTQ Orbitrap Velos ETD hybrid FT mass spectrometer to the Mass Spectrometry Facility at the Medical University of South Carolina. The facility has a 39 year history of offerin state of the art mass spectrometry capability advancing a wide range of biomedical research interests in the local research community. Over the past three years, the facility has provided mass spectrometric analysis and assistance to 77 different faculty investigators within the university and multiple NIH-funded investigators at other institutions. The most highly requested services offered by the facility are protein identification and the characterization of posttranslational modifications. For these purposes, the most heavily used instruments in the facility are the low resolution linear ion trap mass spectrometers. The facility lacks an instrumen with the combined sensitivity, resolution, and multiple modes of peptide fragmentation that are achievable with the LTQ Orbitrap Velos ETD mass spectrometer. In fact, there is not an Orbitrap mass analyzer in South Carolina. The improved mass accuracy of this instrument will provide a higher level of confidence in peptide and protein identifications and in the site-assignment of posttranslational modifications (PTMs). Combining multiple fragmentation options (CID, HCD, ETD) with high mass accuracy measurements will enable quantitative approaches aimed at simultaneous identification and quantitation of protein modifications, a capability which is not currently available to facility users. The ETD ionization source accessory will 1) aid in projects requiring the characterization of PTMs and de novo sequencing and 2) provide new capabilities enabling top to middle-down protein analysis and quantitation of modifications that are destroyed during CID. The requested instrument will serve a representative group of Major Users including eleven NIH-funded investigators conducting research in the areas of cancer, cardiovascular disease, diabetes, vision, osteoporosis, and kidney disease. The Orbitrap Velos ETD instrument incorporates two mass analyzers and multiple fragmentation strategies yielding a powerful and very versatile instrument that will be well suited to address the broad range of protein analyses and characterization requested by investigators at MUSC and the neighboring universities and research centers in South Carolina. PUBLIC HEALTH RELEVANCE: The analysis of proteins by mass spectrometry is a fundamental analytical method utilized in biomedical research aimed at understanding processes underlying the onset and progression of disease and developing new therapeutics and diagnostics. The requested instrumentation will enhance our ability to identify and monitor changes in protein expression and regulatory post-translational modifications thereby advancing research in multiple areas including cancer, cardiovascular disease, diabetes, blindness, neurological disease, and osteoporosis.

描述（由申请人提供）：本提案旨在为南卡罗来纳州医科大学的质谱设施增加Thermo Scientific LTQ Orbitrap Velos ETD混合FT质谱仪。该设施有39年的历史，提供最先进的质谱分析能力，推动了当地研究界广泛的生物医学研究兴趣。在过去的三年里，该设施提供了质谱分析和援助，以77个不同的教师调查人员在大学和多个NIH资助的调查人员在其他机构。该设施提供的最高要求的服务是蛋白质鉴定和翻译后修饰的表征。为此，该设施中使用最多的仪器是低分辨率线性离子阱质谱仪。该设施缺乏具有LTQ Orbitrap Velos ETD质谱仪可实现的组合灵敏度、分辨率和多种肽片段化模式的仪器。事实上，在南卡罗来纳州没有轨道阱质量分析仪。该仪器质量精度的提高将为肽和蛋白质鉴定以及翻译后修饰（PTM）的位点分配提供更高水平的置信度。将多个片段化选项（CID、HCD、ETD）与高质量准确度测量相结合，将实现旨在同时鉴定和定量蛋白质修饰的定量方法，这是设施用户目前无法获得的能力。ETD电离源附件将1）帮助需要PTM表征和从头测序的项目，2）提供新的功能，实现从上到下的蛋白质分析和定量CID期间被破坏的修饰。所要求的仪器将服务于一组具有代表性的主要用户，包括11名NIH资助的研究人员，他们在癌症、心血管疾病、糖尿病、视力、骨质疏松症和肾脏疾病领域进行研究。Orbitrap Velos ETD仪器结合了两个质量分析仪和多种片段化策略，产生了一个功能强大且用途广泛的仪器，非常适合满足MUSC和邻近大学以及南卡罗来纳州研究中心研究人员的广泛蛋白质分析和表征要求。公共卫生关系：通过质谱分析蛋白质是生物医学研究中使用的基本分析方法，旨在了解疾病发生和进展的基础过程，并开发新的治疗和诊断方法。所要求的仪器将增强我们识别和监测蛋白质表达变化和调节翻译后修饰的能力，从而推进癌症、心血管疾病、糖尿病、失明、神经系统疾病和骨质疏松症等多个领域的研究。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

S-Glutathionylated Serine Proteinase Inhibitors as Biomarkers for Radiation Exposure in Prostate Cancer Patients.

S-谷胱甘肽化丝氨酸蛋白酶抑制剂作为前列腺癌患者辐射暴露的生物标志物。

DOI：
10.1038/s41598-019-50288-9
发表时间：
2019
期刊：
Scientific reports
影响因子：
4.6
作者：
Zhang,Leilei;Zhang,Jie;Ye,Zhiwei;Manevich,Yefim;Townsend,DanyelleM;Marshall,DavidT;Tew,KennethD
通讯作者：
Tew,KennethD

In Situ Imaging of Tryptic Peptides by MALDI Imaging Mass Spectrometry Using Fresh-Frozen or Formalin-Fixed, Paraffin-Embedded Tissue.