Proteomics Core

摘要 蛋白质组学核心B的目标是提供最先进的基于质谱的蛋白质组学 能力，专门的分析支持，以及通过SC COBRE资助的对调查人员的指导 氧化剂，氧化还原平衡和应力信号（氧化还原COBRE）。质谱是一种强大的 技术，使公正的发现药物/配体，遗传变异和疾病的影响， 蛋白质表达、蛋白质相互作用和翻译后修饰的变化。翻译后 修饰编码细胞的营养和应激状态，并介导对配体的急性反应 通过影响蛋白质功能、半衰期和定位来刺激和改变氧化还原状态。MUSC 质谱设施和蛋白质组学中心拥有广泛的蛋白质分析仪器 在生物医学研究工作中应用蛋白质组学技术的表征和专业知识。期间 上一个供资期内，通过共享项目购买了一台Orbitrap Elite质谱仪， 仪器补助金，使我们第一次应用定量蛋白质组学方法来检测 复杂的翻译后修饰。在与COBRE调查人员正在进行的合作项目中， Redox中心的参与者，蛋白质组学核心B正在利用Orbitrap Elite来检查全球影响 改变蛋白质表达、磷酸化、乙酰化和巯基修饰的氧化还原状态， 表征磷酸化，S-谷胱甘肽化，酪氨酸硝化和蛋白质交联的位点， 氧化还原敏感的靶蛋白。蛋白质组学核心B的具体目标是：1）协助COBRE研究人员 实验设计包括样品制备策略和特定蛋白质组学方法的选择，2） 定制数据采集和分析，以解决各个项目的目标，以及3）提供 为未来项目提供蛋白质组技术应用方面的指导和培训。访问这些 推动现有技术的能力和专业知识对拟议的研究项目至关重要。 与COBRE调查人员建立的实验能力和基础设施可用于 MUSC和南卡罗来纳州其他机构的研究人员利用MUSC质谱仪 设施。我们的目标是为COBRE调查人员提供仪器和专业知识， 推进他们的研究，并有助于我们有前途的青年教师的成功。

ABSTRACT The objective of Proteomics Core B is to provide state of the art mass spectrometry-based proteomic capabilities, dedicated analytical support, and mentorship to the investigators funded through the SC COBRE in Oxidants, Redox Balance, and Stress Signaling (Redox COBRE). Mass spectrometry is a powerful technology enabling unbiased discovery of the effects of drugs/ligands, genetic alterations, and disease on changes in protein expression, protein interactions, and post-translational modifications. Post-translational modifications encode the nutrient and stress status of the cell and mediate acute responses to ligand stimulation and changes in redox status by influencing protein function, half-life, and localization. The MUSC Mass Spectrometry Facility and Proteomics Center have an extensive array of instrumentation for protein characterization and expertise in applying proteomic technologies to biomedical research endeavors. During the last funding period acquisition of an Orbitrap Elite mass spectrometer, purchased through a shared instrumentation grant, enabled us for the first time to apply quantitative proteomic approaches to the detection of complex post-translational modifications. In ongoing collaborative projects with COBRE investigators and participants in the Redox Center, Proteomics Core B is utilizing the Orbitrap Elite to examine the global effects of altered redox status on protein expression, phosphorylation, acetylation, and thiol modifications and to characterize the sites of phosphorylation, S-glutathionylation, tyrosine nitration, and protein crosslinking on redox-sensitive target proteins. The specific aims of Proteomics Core B are: 1) to assist COBRE investigators with experimental design including sample preparation strategies and choice of specific proteomic approach, 2) to customize data acquisition and analysis to address the goals of the individual projects, and 3) to provide mentorship and training in the applications of proteomic technologies for future projects. Access to these capabilities and expertise to push the available technology forward is critical to the proposed research projects. The experimental capabilities and infrastructure established with COBRE investigators are made available to investigators at MUSC and other institutions in South Carolina that utilize the MUSC Mass Spectrometry Facility. Our goals are to provide COBRE investigators with access to instrumentation and expertise to advance their research and contribute to the success of our promising junior faculty.