Upgrading the CVID Biosensor Core Facility with a Biacore 8K instrument

使用 Biacore 8K 仪器升级 CVID 生物传感器核心设施

• 批准号: 10176937
• 负责人: Dudley K. Strickland
• 金额: $ 43.89万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176937
• 关键词: Attention; Biology; Biosensor; Blood Vessels; Communities; Core Facility; Disease; Experimental Designs; Funding; Genes; Genomics; Home; Inflammatory; Label; Maryland; Measures; Minor; Molecular Weight; Peer Review; Physiological; Proteomics; Publications; Reproducibility; Research; Resources; Surface Plasmon Resonance; System; Technology; Universities; biomedical resource; gene product; innovation; instrument; medical schools

Project Summary/Abstract As tremendous advances have occurred in genomic biology, gene sequencing technologies, and proteomic technologies, attention is increasingly focusing on gene products, most of which function by interacting with other gene products. A comprehensive understanding of physiological systems requires an in-depth definition of quantitative interactions between molecules. Biosensor technologies provides a powerful approach to define these interactions in label-free systems. Here we seek funds to upgrade the Biosensor Core facility in the Center of Vascular and Inflammatory Diseases (CVID) at the University of Maryland School of Medicine by the purchase of a Biacore 8K instrument. The major users of this Core have extensive expertise employing surface plasmon resonance technology with over 45 peer-reviewed publications employing the technology. The new instrument (Biacore 8K) will be integrated into the CVID Biosensor Core Facility and with the Center for Innovative Biomedical Resources (CIBR), the Administrative home for the University of Maryland School of Medicine Core facilities, thus providing the resource for our growing research community. The Biacore 8K will enhance the research of the Major users and Minor users by increasing the throughput capacity, by enhancing a more reproducible and optimized experimental design, and by increasing the sensitivity for measuring the interaction of low molecular weight molecules.

项目摘要/摘要 由于基因组生物学，基因测序技术和 蛋白质组学技术，注意力越来越集中在基因产品上，其中大多数 通过与其他基因产物相互作用来功能。对生理的全面理解 系统需要对分子之间定量相互作用的深入定义。生物传感器 Technologies提供了一种强大的方法来定义无标签系统中的这些相互作用。 在这里，我们寻求资金来升级血管中心的生物传感器核心设施 马里兰大学医学院的炎症性疾病（CVID） Biacore 8K仪器的。该核心的主要用户具有广泛的专业知识 表面等离子体共振技术拥有超过45个同行评审的出版物 技术。新仪器（Biacore 8K）将集成到CVID生物传感器核心中 设施和创新生物医学资源中心（CIBR），行政家庭 马里兰大学医学院核心设施，从而为 我们不断发展的研究社区。 Biacore 8K将增强主要用户的研究 和次要用户通过增加吞吐量的能力，增强更可重复的和 优化的实验设计，并提高了测量相互作用的敏感性 低分子量分子。