Upgrading the CVID Biosensor Core Facility with a Biacore 8K instrument

项目概要/摘要 随着基因组生物学、基因测序技术和 随着蛋白质组技术的发展，人们越来越关注基因产物，其中大部分 与其他基因产物相互作用。全面了解生理 系统需要深入定义分子之间的定量相互作用。生物传感 技术提供了一种强有力的方法来定义无标签系统中的这些相互作用。 在这里，我们寻求资金，以升级血管中心的生物传感器核心设施， 炎症性疾病（CVID）在马里兰州医学院的购买 一台Biacore 8 K仪器该核心的主要用户拥有广泛的专业知识， 表面等离子体共振技术，超过45篇同行评审的出版物采用了 技术.新仪器（Biacore 8 K）将集成到CVID生物传感器核心中 设施和创新生物医学资源中心（CIBR），行政之家 为马里兰州医学院核心设施的大学，从而提供资源， 不断壮大的研究社区。Biacore 8 K将加强主要用户的研究 和小用户通过增加吞吐量的能力，通过增强更可重复的， 优化的实验设计，并通过增加测量的相互作用的灵敏度， 低分子量分子。

Project Summary/Abstract As tremendous advances have occurred in genomic biology, gene sequencing technologies, and proteomic technologies, attention is increasingly focusing on gene products, most of which function by interacting with other gene products. A comprehensive understanding of physiological systems requires an in-depth definition of quantitative interactions between molecules. Biosensor technologies provides a powerful approach to define these interactions in label-free systems. Here we seek funds to upgrade the Biosensor Core facility in the Center of Vascular and Inflammatory Diseases (CVID) at the University of Maryland School of Medicine by the purchase of a Biacore 8K instrument. The major users of this Core have extensive expertise employing surface plasmon resonance technology with over 45 peer-reviewed publications employing the technology. The new instrument (Biacore 8K) will be integrated into the CVID Biosensor Core Facility and with the Center for Innovative Biomedical Resources (CIBR), the Administrative home for the University of Maryland School of Medicine Core facilities, thus providing the resource for our growing research community. The Biacore 8K will enhance the research of the Major users and Minor users by increasing the throughput capacity, by enhancing a more reproducible and optimized experimental design, and by increasing the sensitivity for measuring the interaction of low molecular weight molecules.