Upgrading the CVID Biosensor Core Facility with a Biacore 8K instrument

项目摘要/摘要 随着基因组生物学、基因测序技术和 蛋白质组学技术，人们越来越关注基因产品，其中大部分 通过与其他基因产物相互作用而发挥作用。全面了解生理学 系统需要对分子之间的定量相互作用进行深入的定义。生物传感器 Technologies提供了一种强大的方法来定义无标签系统中的这些交互。 在这里，我们寻求资金来升级血管和医疗中心的生物传感器核心设施 炎症性疾病(CVID)在马里兰大学医学院购买 一台Biacore 8K仪器。此核心的主要用户拥有广泛的专业知识 表面等离子共振技术，有超过45篇同行评议的出版物使用 技术新仪器(Biacore 8K)将被集成到CVID生物传感器核心中 设施，并与创新生物医学资源中心(CIBR)，行政之家 用于马里兰大学医学院的核心设施，从而为 我们不断壮大的研究社区。Biacore 8K将加强主要用户的研究 和较小的用户，通过增加吞吐量，通过增强更多的可重复性和 优化的实验设计，并通过提高测量相互作用的灵敏度 低分子量分子。

Project Summary/Abstract As tremendous advances have occurred in genomic biology, gene sequencing technologies, and proteomic technologies, attention is increasingly focusing on gene products, most of which function by interacting with other gene products. A comprehensive understanding of physiological systems requires an in-depth definition of quantitative interactions between molecules. Biosensor technologies provides a powerful approach to define these interactions in label-free systems. Here we seek funds to upgrade the Biosensor Core facility in the Center of Vascular and Inflammatory Diseases (CVID) at the University of Maryland School of Medicine by the purchase of a Biacore 8K instrument. The major users of this Core have extensive expertise employing surface plasmon resonance technology with over 45 peer-reviewed publications employing the technology. The new instrument (Biacore 8K) will be integrated into the CVID Biosensor Core Facility and with the Center for Innovative Biomedical Resources (CIBR), the Administrative home for the University of Maryland School of Medicine Core facilities, thus providing the resource for our growing research community. The Biacore 8K will enhance the research of the Major users and Minor users by increasing the throughput capacity, by enhancing a more reproducible and optimized experimental design, and by increasing the sensitivity for measuring the interaction of low molecular weight molecules.