BIACORE 3000 SURFACE PLASMON RESONANCE INSTRUMENT

随着人类基因组计划接近完成，人们的注意力又回到了基因上 基因产物，其中大部分通过与其他基因产物或与 配体。因此，理解函数通常需要理解 蛋白质参与的各种相互作用。生物传感器技术提供了 检测和表征蛋白质-蛋白质的最有效方法 交互.在这里，我们要求资金购买Biacore模型3000，以促进 研究血液和基质蛋白及其受体的各种相互作用。 五个主要用户将研究纤连蛋白（英厄姆）的分子相互作用， 纤维蛋白原（Medved）、凝血因子VIII（Saenko）、纤溶酶原激活剂 抑制剂（Lawrence）和低密度脂蛋白受体家族成员 （斯特里克兰）。一个次要用户和一个主要用户也将使用该仪器来帮助 鉴定跨膜受体配体。第二个次要用户将使用 用于表征配体与整联蛋白α M β 2相互作用的仪器。的 该工具的可用性将大大提高这些NIH资助的 研究人员实现他们的目标，并将加快发展， 更好地了解各种病理所需的知识。

As the human genome project nears completion attention is returning to the gene products, most of which function by interacting with other gene products or with ligands. Thus an understanding of function usually requires an understanding of the various interactions that a protein engages in. Biosensor technology offers one of the most powerful approaches to detecting and characterizing protein-protein interactions. Here we request funds to purchase a Biacore model 3000 to facilitate studies of a variety of interactions of blood and matrix proteins and their receptors. Five major users will investigate molecular interactions of fibronectin (Ingham), fibrinogen (Medved), coagulation factor VIII (Saenko), plasminogen activator inhibitors (Lawrence), and members of the low-density lipoprotein receptor family (Strickland). One minor and one major user will also use the instrument to help identify ligands for transmembrane receptors. A second minor user will use the instrument to characterize ligand interactions with integrin alphaMbeta2. The availability of this instrument will greatly enhance the ability of these NIH-funded investigators to achieve their aims and will accelerate the development of knowledge required to better understand a variety of pathologies.