Regional 800 MHz NMR System Upgrade

7.项目摘要 该提案旨在为最先进的800 MHz NMR射频（RF）控制台提供资金，[1H/19 F，13 C，15 N，2 H]- 冷冻探针、冷冻探针冷却单元和超导磁体泵站控制器和监测单元， 以及温控进样器附件，针对现代解决方案状态进行了优化配置 生物分子核磁共振这种替代NMR控制台的主要需求是满足以下关键需求： 在伦斯勒理工学院广泛且不断增长的用户群中收集800 MHz的NMR数据 (RPI)，以及纽约北部和新泽西地区的其他机构。这个核磁共振光谱仪 该系统将取代15年前Bruker Avance II（AVII）600 MHz NMR控制台、冷冻探针和磁力泵 制造商不再支持的控制器系统，并且其承载能力有限 我们不断增长的NIH资助的NMR用户社区所需的现代NMR实验。该替换RF 控制台和探头，以及现有的维护良好的主动屏蔽18.8特斯拉超导 磁铁与US 2低温恒温器，将形成一个集成的生物分子核磁共振系统。该提案是在 代表大约21个研究小组，包括14个NIH资助的小组，将利用拟议的NMR 测量仪器。现有AV II控制台由于有限的 更换部件的可用性，以及无法运行现代NMR数据收集软件。校长 用户群体包括生物技术和跨学科科学中心的教师，以及 RPI的生物学、化学和化学生物学以及化学工程系，以及外部用户 团体，包括奥尔巴尼的纽约州立大学，纽约的州立大学， 布法罗、奥尔巴尼药学与健康科学学院、锡耶纳学院和罗格斯大学-该州 新泽西大学。这些研究人员跨越了在NMR方面专业知识有限的小组， 和/或学生的数据收集和分析工作的设施，以高度经验丰富的生物分子NMR 需要访问高场NMR数据收集的光谱学家。RPI 800 MHz频谱仪具有 作为纽约首都地区的重要资源，在过去的14年里， 在特洛伊120英里范围内有这种或更强的磁场系统。硬件急需升级， 存在不可修复的硬件故障的可能性，并且使用它的研究小组无法执行他们的任务。 美国国立卫生研究院资助的研究没有最先进的800 MHz核磁共振系统。他们资助的项目已经成熟， 将在安装后立即使用新硬件。升级后的文书将产生广泛的影响， 核磁共振的大部分时间将用于技术开发和广泛的合作 活动负责这个核磁共振核心设施的科学家的杰出专业知识， 管理和维护政策，承诺支持主要和次要用户， 内部和外部用户的费率将确保这一工具作为区域资源取得成功。 1

7. Project Summary This proposal seeks to fund a state-of-the-art 800 MHz NMR radio frequency (RF) console, [1H/19F,13C,15N,2H]- cryoprobe, cryoprobe cooling unit, and superconducting magnet pump station controller and monitoring unit, as well as a temperature-controlled sample changer accessory, optimally configured for modern solution-state biomolecular NMR. The primary need for this replacement NMR console is to address the critical need for NMR data collection at 800 MHz among a broad and growing user group at Rensselaer Polytechnic Institute (RPI), and at other institutions in the upstate New York and New Jersey region. This NMR spectrometer system will replace a 15-Yr old Bruker Avance II (AVII) 600 MHz NMR console, cryoprobe, and magnet pump controller system that are no longer supported by the manufacturer, and which have limited capabilities to carry out modern NMR experiments required by our growing NIH-funded NMR user community. The replacement RF console and probe, together with an existing well-maintained actively-shielded 18.8 Tesla superconducting magnet with US2 cryostat, will form an integrated biomolecular NMR system. This proposal was prepared on behalf of some 21 research groups, including 14 NIH-funded groups, that will utilize the proposed NMR instrument for measurements. This existing AV II console cannot be sustainably maintained due to limited availability of replacement parts, and its inability to run modern NMR data collection software. The principal user groups include faculty at the Center for Biotechnology and Interdisciplinary Sciences, and in the Departments of Biology, Chemistry and Chemical Biology, and Chemical Engineering at RPI, and external user groups, including faculty of the State University of New York at Albany, the State University of New York at Buffalo, Albany College of Pharmacy and Health Sciences, Sienna College, and Rutgers – The State University of New Jersey. These researchers span groups with limited expertise in NMR who send samples and/or students to the facility for data collection and analysis efforts, to highly-experienced biomolecular NMR spectroscopists who need access to high field NMR data collection. The RPI 800 MHz spectrometer has served for more than 14 years as a critical resource to the capital region of New York, with no other NMR system of this field strength or greater within 120 miles of Troy. The hardware is in urgent need of upgrade, as there is the potential for irreparable hardware failure, and the research groups who use it cannot carry out their NIH-funded research without a state-of-the-art 800 MHz NMR system. Their funded projects are mature, and will utilize new hardware as soon as it is installed. The upgraded instrument will have broad impact, as a significant portion of NMR time will be dedicated to technology development and a broad range of collaborative activities. The outstanding expertise of the scientists responsible for this NMR Core Facility, well-established administration and maintenance policies, commitment to support both major and minor users, and affordable rates for both internal and external users will ensure success of this instrument as a regional resource. 1