Regional 800 MHz NMR System Upgrade

7.项目总结 这项提案旨在为最先进的800 MHz核磁共振射频(RF)控制台提供资金，[1H/19F，13C，15N，2H]- 低温探头、低温探头冷却单元、超导磁体泵站控制器和监测单元，AS 以及温度控制的样品转换器附件，为现代溶液状态进行了优化配置 生物分子核磁共振。更换此核磁共振控制台的主要需求是满足以下关键需求 在伦斯勒理工学院不断增长的广泛用户群体中以800 MHz的频率收集核磁共振数据 (RPI)，以及纽约州北部和新泽西州地区的其他机构。这台核磁共振波谱仪 系统将取代已有15年历史的布鲁克Avance II(AVII)600 MHz核磁共振控制台、低温探头和磁力泵 不再受制造商支持且承载能力有限的控制器系统 我们不断增长的NIH资助的核磁共振用户社区所需的现代核磁共振实验。替代的射频 控制台和探测器，以及现有维护良好的主动屏蔽18.8特斯拉超导 磁体与US2低温恒温器，将形成一个完整的生物分子核磁共振系统。这份提案是在 代表大约21个研究小组，包括14个由美国国立卫生研究院资助的小组，将利用拟议的核磁共振 用于测量的仪器。现有的AV II游戏机由于有限的限制而无法持续维护 更换部件的可用性，以及无法运行现代核磁共振数据收集软件。校长 用户群体包括生物技术和跨学科科学中心的教员，以及 RPI的生物、化学和化学生物学、化学工程系和外部用户 团体，包括纽约州立大学奥尔巴尼分校、纽约州立大学奥尔巴尼分校 布法罗、奥尔巴尼药学和健康科学学院、西耶纳学院和罗格斯州立大学 新泽西大学。这些研究人员跨越了在核磁共振方面专业知识有限的小组，他们发送样本 和/或学生到设施进行数据收集和分析工作，到经验丰富的生物分子核磁共振 需要获取高场核磁共振数据收集的光谱学家。RPI 800 MHz光谱仪具有 作为纽约首都地区的关键资源服务了14年以上，没有其他核磁共振 特洛伊方圆120英里范围内的这种场强或更大的系统。硬件急需升级，因为 存在无法修复的硬件故障的可能性，使用它的研究小组无法执行他们的 NIH资助的研究没有最先进的800 MHz核磁共振系统。他们资助的项目已经成熟，而且 将在安装后立即使用新硬件。升级后的仪器将产生广泛的影响，作为 大部分核磁共振时间将专门用于技术开发和广泛的协作 活动。负责这个核磁共振核心设备的科学家的杰出专业知识， 管理和维护政策、支持主要用户和次要用户的承诺以及经济实惠 内部和外部用户的费率将确保这一工具作为一项区域资源取得成功。 1

7. Project Summary This proposal seeks to fund a state-of-the-art 800 MHz NMR radio frequency (RF) console, [1H/19F,13C,15N,2H]- cryoprobe, cryoprobe cooling unit, and superconducting magnet pump station controller and monitoring unit, as well as a temperature-controlled sample changer accessory, optimally configured for modern solution-state biomolecular NMR. The primary need for this replacement NMR console is to address the critical need for NMR data collection at 800 MHz among a broad and growing user group at Rensselaer Polytechnic Institute (RPI), and at other institutions in the upstate New York and New Jersey region. This NMR spectrometer system will replace a 15-Yr old Bruker Avance II (AVII) 600 MHz NMR console, cryoprobe, and magnet pump controller system that are no longer supported by the manufacturer, and which have limited capabilities to carry out modern NMR experiments required by our growing NIH-funded NMR user community. The replacement RF console and probe, together with an existing well-maintained actively-shielded 18.8 Tesla superconducting magnet with US2 cryostat, will form an integrated biomolecular NMR system. This proposal was prepared on behalf of some 21 research groups, including 14 NIH-funded groups, that will utilize the proposed NMR instrument for measurements. This existing AV II console cannot be sustainably maintained due to limited availability of replacement parts, and its inability to run modern NMR data collection software. The principal user groups include faculty at the Center for Biotechnology and Interdisciplinary Sciences, and in the Departments of Biology, Chemistry and Chemical Biology, and Chemical Engineering at RPI, and external user groups, including faculty of the State University of New York at Albany, the State University of New York at Buffalo, Albany College of Pharmacy and Health Sciences, Sienna College, and Rutgers – The State University of New Jersey. These researchers span groups with limited expertise in NMR who send samples and/or students to the facility for data collection and analysis efforts, to highly-experienced biomolecular NMR spectroscopists who need access to high field NMR data collection. The RPI 800 MHz spectrometer has served for more than 14 years as a critical resource to the capital region of New York, with no other NMR system of this field strength or greater within 120 miles of Troy. The hardware is in urgent need of upgrade, as there is the potential for irreparable hardware failure, and the research groups who use it cannot carry out their NIH-funded research without a state-of-the-art 800 MHz NMR system. Their funded projects are mature, and will utilize new hardware as soon as it is installed. The upgraded instrument will have broad impact, as a significant portion of NMR time will be dedicated to technology development and a broad range of collaborative activities. The outstanding expertise of the scientists responsible for this NMR Core Facility, well-established administration and maintenance policies, commitment to support both major and minor users, and affordable rates for both internal and external users will ensure success of this instrument as a regional resource. 1