Regional 800 MHz NMR System Upgrade

区域 800 MHz NMR 系统升级

• 批准号: 10177120
• 负责人: GAETANO T MONTELIONE
• 金额: $ 131.52万
• 依托单位: RENSSELAER POLYTECHNIC INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177120
• 关键词: Address; Biology; Biomolecular Nuclear Magnetic Resonance; Biotechnology; Buffaloes; Capital; Chemical Engineering; Chemicals; Chemistry; Communities; Computer software; Core Facility; Data Analyses; Data Collection; Ensure; Faculty; Failure; Funding; Health Sciences; Institutes; Institution; Maintenance; Manufacturer Name; Measurement; Minor; Modernization; Monitor; NMR Spectroscopy; New Jersey; New York; Pharmacy facility; Policies; Pump; Research; Research Personnel; Resources; Running; Sampling; Science; Scientist; Students; System; Temperature; Time; United States National Institutes of Health; Universities; college; cryostat; experience; experimental study; instrument; radio frequency; success; technology development

7. Project Summary This proposal seeks to fund a state-of-the-art 800 MHz NMR radio frequency (RF) console, [1H/19F,13C,15N,2H]- cryoprobe, cryoprobe cooling unit, and superconducting magnet pump station controller and monitoring unit, as well as a temperature-controlled sample changer accessory, optimally configured for modern solution-state biomolecular NMR. The primary need for this replacement NMR console is to address the critical need for NMR data collection at 800 MHz among a broad and growing user group at Rensselaer Polytechnic Institute (RPI), and at other institutions in the upstate New York and New Jersey region. This NMR spectrometer system will replace a 15-Yr old Bruker Avance II (AVII) 600 MHz NMR console, cryoprobe, and magnet pump controller system that are no longer supported by the manufacturer, and which have limited capabilities to carry out modern NMR experiments required by our growing NIH-funded NMR user community. The replacement RF console and probe, together with an existing well-maintained actively-shielded 18.8 Tesla superconducting magnet with US2 cryostat, will form an integrated biomolecular NMR system. This proposal was prepared on behalf of some 21 research groups, including 14 NIH-funded groups, that will utilize the proposed NMR instrument for measurements. This existing AV II console cannot be sustainably maintained due to limited availability of replacement parts, and its inability to run modern NMR data collection software. The principal user groups include faculty at the Center for Biotechnology and Interdisciplinary Sciences, and in the Departments of Biology, Chemistry and Chemical Biology, and Chemical Engineering at RPI, and external user groups, including faculty of the State University of New York at Albany, the State University of New York at Buffalo, Albany College of Pharmacy and Health Sciences, Sienna College, and Rutgers – The State University of New Jersey. These researchers span groups with limited expertise in NMR who send samples and/or students to the facility for data collection and analysis efforts, to highly-experienced biomolecular NMR spectroscopists who need access to high field NMR data collection. The RPI 800 MHz spectrometer has served for more than 14 years as a critical resource to the capital region of New York, with no other NMR system of this field strength or greater within 120 miles of Troy. The hardware is in urgent need of upgrade, as there is the potential for irreparable hardware failure, and the research groups who use it cannot carry out their NIH-funded research without a state-of-the-art 800 MHz NMR system. Their funded projects are mature, and will utilize new hardware as soon as it is installed. The upgraded instrument will have broad impact, as a significant portion of NMR time will be dedicated to technology development and a broad range of collaborative activities. The outstanding expertise of the scientists responsible for this NMR Core Facility, well-established administration and maintenance policies, commitment to support both major and minor users, and affordable rates for both internal and external users will ensure success of this instrument as a regional resource. 1

7。项目摘要 该建议旨在为最先进的800 MHz NMR射频（RF）控制台提供资金，[1H/19F，13C，15N，2H] - 冷冻探针，冷冻探针冷却单元以及超导磁铁泵站控制器和监视单元， 以及温度控制的样品更换器附件，适用于现代溶液状态的最佳配置 生物分子NMR。此替换NMR控制台的主要需求是满足至关重要的需求 Rensselaer理工学院的广泛且不断增长的用户组中的NMR数据收集在800 MHz （RPI）以及纽约州北部和新泽西州地区的其他机构。该NMR光谱仪 系统将替换15年的旧布鲁克avance II（AVII）600 MHz NMR控制台，冷冻机和磁铁泵 不再得到制造商支持并且能力有限的控制器系统 我们不断增长的NIH资助的NMR用户社区所需的现代NMR实验。替换RF 控制台和探测器，以及现有的良好维护良好的积极速度18.8特斯拉超导 带有US2低温恒温器的磁铁将形成一个集成的生物分子NMR系统。该提议是根据 代表大约21个研究小组，包括14个由NIH资助的小组，这些小组将利用拟议的NMR 测量工具。由于有限 替换零件的可用性及其无法运行现代NMR数据收集软件。校长 用户组包括生物技术与跨学科科学中心的教职员工， RPI的生物学，化学和化学生物学和化学工程部门以及外部用户 小组，包括纽约州立大学奥尔巴尼，纽约州立大学的纽约州立大学 布法罗，奥尔巴尼药房和健康科学学院，锡耶纳学院和罗格斯 - 州 新泽西大学。这些研究人员跨越了发送样品的NMR专业知识有限的小组 和/或学生到该设施进行数据收集和分析工作，以进行高度经验的生物分子NMR 需要访问高场NMR数据收集的光谱学家。 RPI 800 MHz光谱仪具有 为纽约首都地区的关键资源服务了14年以上，没有其他NMR 该野外强度的系统或特洛伊120英里以内的系统。硬件迫切需要升级，因为 有可能无法弥补的硬件故障，使用它的研究小组无法执行 NIH资助的研究没有最先进的800 MHz NMR系统。他们资助的项目已经成熟， 安装后，将立即使用新硬件。升级的仪器将产生广泛的影响，作为 NMR时间的很大一部分将致力于技术开发和广泛的协作 活动。负责此NMR核心设施的科学家的杰出专业知识，已建立了 管理和维护政策，支持主要用户和未成年人的承诺以及负担得起的 内部和外部用户的价格都将确保该工具作为区域资源的成功。 1

项目成果

SpecDB: A relational database for archiving biomolecular NMR spectral data.

• DOI: 10.1016/j.jmr.2022.107268
• 发表时间: 2022-09
• 期刊: JOURNAL OF MAGNETIC RESONANCE
• 影响因子: 2.2
• 作者: Fraga, Keith J.;Huang, Yuanpeng J.;Ramelot, Theresa A.;Swapna, G. V. T.;Kendary, Arwin Lashawn Anak;Li, Ethan;Korf, Ian;Montelione, Gaetano T.
• 通讯作者: Montelione, Gaetano T.