Structural Genomics of Eukaryotic Domain Families

真核域家族的结构基因组学

• 批准号: 8255665
• 负责人: GAETANO T MONTELIONE
• 金额: $ 55.44万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8255665
• 关键词: Amino Acid Sequence; Area; Base Sequence; Binding; Bioinformatics; Biological; Biological Sciences; Biology; Biomedical Research; Cancer Biology; Cells; Cereals; Chemicals; Collaborations; Communities; Complement; Complex; Computer software; Computing Methodologies; Consensus; Coupling; Crystallization; Data; Databases; Deposition; Developmental Biology; Disease; Disulfides; Education; Educational workshop; Engineering; Escherichia coli; Family; Funding; Genomics; Germ Cells; Goals; Grant; Health; Homology Modeling; Human; Hybrids; Information Dissemination; Insect Proteins; Institutes; Ligands; Light; Literature; Malignant Neoplasms; Methods; Mission; Modeling; Molecular Chaperones; NIH Program Announcements; National Institute of General Medical Sciences; Nature; Nuclear; Ontario; Peptide Sequence Determination; Phase; Principal Investigator; Process; Production; Protein Structure Initiative; Proteins; Proteome; Protocols documentation; Publications; Reagent; Relaxation; Research; Research Infrastructure; Research Project Grants; Residual state; Resources; Roentgen Rays; Sampling; Scientist; Sequence Analysis; Solubility; Source; Structure; Synchrotrons; System; Technology; Tertiary Protein Structure; Training; United States National Institutes of Health; Universities; Wheat; X-Ray Crystallography; base; biological research; computerized data processing; design and construction; expression vector; improved; instrument; knowledge base; meetings; method development; monomer; new technology; next generation; novel; programs; protein complex; protein expression; protein structure; repository; structural biology; structural genomics; success; technology development; three dimensional structure; tool; web site

DESCRIPTION (provided by applicant): The Northeast Structural Genomics Consortium (NESG) is one of four Large-Scale Centers (LSCs) for structure production funded by the NIH NIGMS Protein Structure Initiative (PSI). The goals of the PSI LSCs are to (i) generate three-dimensional (3D) structures for large numbers of proteins selected using broad biological, genomic, and bioinformatics criteria, together with targets selected from specific biological theme projects, so as to provide significant structural coverage of a large number of protein sequences In nature, (ii) develop and disseminate novel and/or improved technologies for structural biology and bioinformatics, and (iii) make these structures, structure production data, and the associated reagents and technologies publicly available to the worldwide scientific community. In PSI:Biology, the next phase of the PSI program, the NESG will expand Its mission by carrying our collaborative structural genomics projects together with several PSI Consortia for High-Through-Put (HTP) Enabled Structural Biology Partnerships (Biology Partnerships) and associated Program Announcements (PARs). The primary goal of the NESG In PSI:Biology is to provide > 1,100 new 3D protein structures to the Protein Data Bank (PDB) over 5 years, together with extensive raw and processed data, protocols for sample production, structure/function annotations, and thousands of homology models derived from these structures. This will complement the ~ 900 structures deposited by NESG in PSI Phases 1 and 2. In particular, NESG will provide novel 3D structural information useful in modeling large numbers of eukaryotic and human proteins. Our efforts will span five classes of target types: (i) proteins nominated In collaborations to be established with PSI Biology Partnerships, (ii) domain families (referred to as BIG, MEGA, and META families) defined by the central PSI:Biology Target Selection Subcommittee to provide course-grained coverage of large protein domain families; (iii) proteins defined by the NESG Biomedical Theme of 'Networks of Proteins Associated with Human Cancer and Developmental Biology'; (iv) proteins nominated by the general biomedical research community, and (v) proteins selected for specific technology-development goals. Protein targets in the first two of these classes, representing ~ 80% of the overall NESG effort, will be selected in a coordinated process together with the other LSCs and Biology Partnerships so as to maximize biological impact and minimize redundant efforts. The many methods and technologies for structural genomics research developed in this project will provide the next- generation tools for traditional hypothesis-driven biological research, and will thus have powerful and broad impact on the infrastructure for biological science and engineering.

描述（由申请人提供）：东北结构基因组学联盟（NESG）是由NIH NIGMS蛋白质结构倡议（PSI）资助的结构生产的四个大型中心（LSC）之一。 PSI LSC的目标是（i）生成三维（3D）结构，用于使用广泛的生物学，基因组和生物信息学标准选择的大量蛋白质，以及从特定的生物学主题项目中选择的目标，以便为大量的蛋白质序列提供了大量的蛋白质序列，或改善了（II），（II），（ii），（ii ii），（ii ii），（ii ii），（ii ii），（ii ii），（ii ii ii ii diseminatient of（II） （iii）制造这些结构，结构生产数据以及全球科学界公开可用的相关试剂和技术。在PSI：生物学，PSI计划的下一阶段，NESG将通过携带我们的协作结构基因组学项目以及几个PSI高通用PUT（HTP）启用的结构生物学伙伴关系（生物学伙伴关系）和相关计划公告（PARS）来扩展其任务。 NESG在PSI：生物学中的主要目标是在5年内为蛋白质数据库（PDB）提供> 1,100个新的3D蛋白质结构，以及广泛的原始和加工数据，样品生产的协议，结构/功能注释的协议，以及数千种来自这些结构的同源模型。这将补充NESG在PSI阶段1和2中沉积的〜900个结构。特别是，NESG将提供新颖的3D结构信息，可用于建模大量真核和人类蛋白质。我们的努力将跨越五类的目标类型：（i）与PSI生物学合作伙伴关系建立的合作中提名的蛋白质，（ii）域家族（称为大型，巨型和元家庭）由中央PSI定义的：生物学目标选择小组委员会定义的小组委员会，以提供大型蛋白质领域的课程覆盖范围； （iii）由NESG生物医学主题定义的蛋白质“与人类癌症和发育生物学相关的蛋白质网络”； （iv）由一般生物医学研究界提名的蛋白质，以及（v）用于特定技术开发目标的蛋白质。在这些类别的前两个中，蛋白质靶标（占NESG总体努力的约80％）将在协调过程中与其他LSC和生物学合作伙伴关系中选择，以最大程度地发挥生物学影响并最大程度地减少冗余努力。该项目开发的结构基因组学研究的许多方法和技术将为传统假设驱动的生物学研究提供下一代工具，因此将对生物科学和工程的基础设施产生强大而广泛的影响。