Minnesota HHEAR Targeted Analysis Laboratory

项目总结/摘要：总体 明尼苏达州HHEAR目标分析实验室汇集了来自明尼苏达大学的研究人员， 明尼苏达州和明尼苏达州卫生部拥有广泛的和国际公认的专业知识， 识别和分析对所有年龄段的人类健康有潜在危害的物质。相结合 我们实验室的专业知识是独一无二的。我们的科学家有多年的开发经验 以及主要基于质量的最新高通量分析化学方法的应用 用于评估大量目标毒物或致癌物代谢物的痕量光谱法 临床和流行病学研究。我们的科学家还开发和应用了分析方法， 人类样本中致癌物质的DNA加合物。很少有团体能将一个 我们的团队在多个相关级别的暴露评估方面拥有如此高超和创造性的专业知识。因此，我们认为， 我们的总体目标是为人类健康研究人员提供最先进的基础设施， 生物样品的目标分析。我们认为，这一目标牢固地植根于 疾病及其与人类健康的关系。每个组成资源的具体目标和 发展核心如下。 目标分析资源将主要使用高通量质谱方法， 完善的质量控制程序，提供与3个主要方面相关的及时可靠的定量数据 对人类健康具有重要意义的领域：接触烟草特有的化合物;接触环境， 生活方式，营养毒物和致癌物;以及内源性亲电体和 膳食化合物。 开发核心将建立新的高通量方法来测量多种类别的有毒物质 以及尿液、血浆、头发和脚趾甲中的生物标志物;从食物摄入中检测营养生物标志物 以及尿液和血浆中的饮料;开发检测一组应激的内部生物标志物的测定方法， 多个生物标本;并扩大我们的方法，以检测唾液中的DNA加合物，血液和福尔马林固定 组织中 行政核心将协调明尼苏达州HHEAR目标分析内的所有活动 实验室此外，该核心也将是与HHEAR协调和数据中心的主要链接 作为HHEAR调查员参与项目的各个阶段。 总的来说，明尼苏达州HHEAR目标分析实验室将有助于一个富有成效的和有效的 HHEAR网络旨在了解环境暴露对人类健康的影响。

PROJECT SUMMARY/ABSTRACT: OVERALL The Minnesota HHEAR Targeted Analysis Laboratory brings together investigators from the University of Minnesota and the Minnesota Department of Health with broad and internationally recognized expertise in the identification and analysis of agents that are potentially harmful to human health at all ages. The combination of expertises in our proposed laboratory is unique. Our scientists have years of experience in the development and application of state-of-the-art high throughput analytical chemistry methods based mainly on mass spectrometry for the assessment of trace amounts of targeted toxicant or carcinogen metabolites in large clinical and epidemiological studies. Our scientists also have developed and applied methods for the analysis of DNA adducts of cancer-causing agents in human samples. There are few groups that can bring together a team with such superb and creative expertise on multiple relevant levels of exposure assessment. Therefore, our overall goal is to provide human health researchers with access to state-of-the-art infrastructure for targeted analysis of biological samples. We see this goal as firmly embedded within the concept of the exposome and its relationship to human health. The specific aims of each component resource and the development core are as follows. The Targeted Analysis Resource will use predominantly high throughput mass spectrometric methods with well-established quality control procedures to provide timely and reliable quantitative data relevant to 3 major areas of importance to human health: Exposure to tobacco-specific compounds; exposure to environmental, lifestyle, and nutritional toxicants and carcinogens; and variations in levels of endogenous electrophiles and dietary compounds. The Developmental Core will establish new high throughput methods to measure multiple classes of toxicants and biomarkers in urine, plasma, hair, and toenails; to detect nutritional biomarkers from the intake of foods and beverages in urine and plasma; to develop assays to detect a panel of internal biomarkers of stress in multiple biospecimens; and expand our approaches to detect DNA adducts in saliva, blood and formalin-fixed tissues. The Administrative Core will coordinate all activities within the Minnesota HHEAR Targeted Analysis Laboratory. In addition, this core will be the primary link to the HHEAR Coordinating and Data Centers as well as the HHEAR investigators at all stages of their projects. Collectively, the Minnesota HHEAR Targeted Analysis Laboratory will contribute to a productive and effective HHEAR network designed to understand the impact of environmental exposures on human health.