Minnesota HHEAR Targeted Analysis Laboratory

项目摘要/摘要：总体 明尼苏达州HHEAR目标分析实验室汇集了来自加州大学的研究人员 明尼苏达州和明尼苏达州卫生部在 识别和分析对所有年龄段的人类健康都有潜在危害的制剂。组合在一起 在我们拟建的实验室中，专家的数量是独一无二的。我们的科学家在开发方面有多年的经验 以质量为主的最新高通量分析化学方法及其应用 用于评估大量目标毒物或致癌代谢物的痕量光谱分析 临床和流行病学研究。我们的科学家还开发和应用了分析方法。 人体样本中致癌物质的DNA加合物。几乎没有几个团体可以将一个 在多个相关级别的暴露评估方面拥有如此精湛和创造性的专业知识的团队。因此， 我们的总体目标是为人类健康研究人员提供访问最先进的基础设施的途径 生物样本的靶向分析。我们认为这一目标牢牢植根于 曝光组及其与人类健康的关系。每种组件资源的具体目标和 发展的核心内容如下。 目标分析资源将主要使用高通量质谱学方法 建立完善的质量控制程序，提供与3个主要项目相关的及时可靠的量化数据 对人类健康很重要的领域：接触烟草特有的化合物；接触环境， 生活方式、营养毒物和致癌物；以及内源性亲电体和 饮食化合物。 开发核心将建立新的高通量方法来测量多种毒物 以及尿液、血浆、头发和脚趾甲中的生物标记物；检测食物摄入量中的营养生物标记物 以及尿液和血浆中的饮料；开发检测一组体内应激生物标志物的方法 多种生物显微镜；并扩大我们的方法，以检测唾液、血液和福尔马林固定的DNA加合物 纸巾。 行政核心将协调明尼苏达州HHEAR目标分析中的所有活动 实验室。此外，这一核心也将是HHEAR协调和数据中心的主要链接 作为HHEAR调查人员在他们项目的所有阶段。 总体而言，明尼苏达州HHEAR目标分析实验室将有助于 HHEAR网络旨在了解环境暴露对人类健康的影响。

PROJECT SUMMARY/ABSTRACT: OVERALL The Minnesota HHEAR Targeted Analysis Laboratory brings together investigators from the University of Minnesota and the Minnesota Department of Health with broad and internationally recognized expertise in the identification and analysis of agents that are potentially harmful to human health at all ages. The combination of expertises in our proposed laboratory is unique. Our scientists have years of experience in the development and application of state-of-the-art high throughput analytical chemistry methods based mainly on mass spectrometry for the assessment of trace amounts of targeted toxicant or carcinogen metabolites in large clinical and epidemiological studies. Our scientists also have developed and applied methods for the analysis of DNA adducts of cancer-causing agents in human samples. There are few groups that can bring together a team with such superb and creative expertise on multiple relevant levels of exposure assessment. Therefore, our overall goal is to provide human health researchers with access to state-of-the-art infrastructure for targeted analysis of biological samples. We see this goal as firmly embedded within the concept of the exposome and its relationship to human health. The specific aims of each component resource and the development core are as follows. The Targeted Analysis Resource will use predominantly high throughput mass spectrometric methods with well-established quality control procedures to provide timely and reliable quantitative data relevant to 3 major areas of importance to human health: Exposure to tobacco-specific compounds; exposure to environmental, lifestyle, and nutritional toxicants and carcinogens; and variations in levels of endogenous electrophiles and dietary compounds. The Developmental Core will establish new high throughput methods to measure multiple classes of toxicants and biomarkers in urine, plasma, hair, and toenails; to detect nutritional biomarkers from the intake of foods and beverages in urine and plasma; to develop assays to detect a panel of internal biomarkers of stress in multiple biospecimens; and expand our approaches to detect DNA adducts in saliva, blood and formalin-fixed tissues. The Administrative Core will coordinate all activities within the Minnesota HHEAR Targeted Analysis Laboratory. In addition, this core will be the primary link to the HHEAR Coordinating and Data Centers as well as the HHEAR investigators at all stages of their projects. Collectively, the Minnesota HHEAR Targeted Analysis Laboratory will contribute to a productive and effective HHEAR network designed to understand the impact of environmental exposures on human health.