Epigenetic & Genomics

项目摘要核心C 糖尿病研究的一个主要目标是了解表观基因组和随后的 基因表达的反应影响疾病的表型和治疗方案。该计划的目标是 表观遗传学和基因组学核心(EGC)是提供尖端、可靠和创新的基因组技术 支持刚果民主共和国研究人员的糖尿病及相关内分泌和代谢研究目标。它还 提供基因组学技术方面的培训、教育和咨询，以提高刚果民主共和国的能力 调查人员在他们的研究中实施这些技术。教育统筹局将提供下列服务： 测序分析的技术支持：核心将为高 在Illumina测序平台(MiSeq、HiSeq2500、HiSeq4000、NovaSeq)上进行吞吐量测序分析 6000)、启用RNA测序(RNA-seq)、低输入RNA-seq、microRNA测序(MiRNAseq)、全球 连续测序(Gro-Seq)、核糖体分析和深度测序(Ribo-Seq)、染色质 免疫沉淀与大规模平行测序(CHIP-SEQ)，转座酶可及测序 高通量测序染色质(ATAC-Seq)，交联免疫沉淀(CLIP-Seq)，Hi-C， CRISPR筛查的甲基C测序、元基因组分析和测序。 单细胞测序分析：核心将为单细胞测序分析提供技术支持， 包括10倍基因组铬单细胞解决方案技术，以及新技术的实施 当他们出现的时候。 培训和咨询：核心将为学生、博士后研究员、 关于高通量测序方法和数据分析的研究人员和技术人员。 这些职能将由功能基因组学专家监督。 生物信息学支持：核心将提供生物信息学支持，以帮助进行实验 设计、技术平台选择、数据分析和数据质量控制。

Project Summary Core C A major goal in diabetes research is to understand how alterations in the epigenome and subsequent responses in gene expression impact disease phenotype and treatment regimens. The objective of the Epigenetic and Genomics Core (EGC) is to provide cutting-edge, reliable and innovative genomic technologies to support the diabetes and related endocrinology and metabolism research goals of DRC investigators. It also offers training, education and consultation in genomics technologies in order to enhance the ability of DRC Investigators to implement these technologies in their research. The EGC will provide the following services: Technical Support For Sequencing-Based Assays: The Core will provide technical support for high- throughput sequencing assays on Illumina sequencing platforms (MiSeq, HiSeq2500, HiSeq4000, NovaSeq 6000), enabling RNA sequencing (RNA-seq), low-input RNA-seq, microRNA sequencing (miRNAseq), Global Run- On sequencing (GRO-Seq), ribosome profiling and deep sequencing (Ribo-Seq), Chromatin Immunoprecipitation linked to massively parallel sequencing (ChIP-Seq), Assay for Transposase- Accessible Chromatin with high throughput sequencing (ATAC-Seq), Cross-Linking Immunoprecipitation (CLIP-Seq), Hi-C, MethylC-sequencing, metagenomic assays, and sequencing of CRISPR Screens. Single Cell Sequencing Assays: The Core will provide technical support for single cell sequencing assays, including 10X Genomics Chromium Single Cell Solution technologies, and implementation of new technologies as they arise. Training and Consultation: The Core will provide consultation and training of students, postdoctoral fellows, investigators and technical staff regarding high-throughput sequencing methodologies and data analysis. These functions will be overseen by the Functional Genomics Specialist. Bioinformatics Support: The Core will provide bioinformatics support for assistance with experimental design, choice of technological platform, data analysis and data quality control.