ShEEP Request for Enabling 3D Nano-Printer Technology

项目摘要/摘要 所要求的仪器是先进的3D打印系统，用于生物应用，克服了 传统添加剂制造印刷设备的许多长度-比例限制。仪器仪表 利用直接分子打印和紫外光能量转移两种串联技术开发 统一的、高度可控的、具有分辨率和长度刻度的生物工程设备 纳米到微米的范围。该仪器的设计目的是产生可控制的分子阵列 研究受体-配体、蛋白质-蛋白质或酶-抑制剂的相互作用，具体应用于：1) 组织工程，2)影响细胞分化和功能的基于细胞的研究，3)药物发现和 4)微生物学。该仪器的主要目标是制造新型生物工程设备，包括 多肽、糖蛋白或寡核苷酸的阵列；精确定义的微流控通道；以及 或几何定制的生物功能芯片，以推进上面列出的涵盖退伍军人管理局资助的研究领域 南亚利桑那州退伍军人医疗保健系统(SAVAHCS)的研究。此外，3D纳米打印系统 可能会被亚利桑那大学学术附属机构的调查人员和其他合作者访问 这将扩大仪器的影响并加强研究合作， 特别是在附近的两个医疗中心之间，以及整个西南部地区。

PROJECT SUMMARY/ABSTRACT The requested instrumentation is an advanced 3D printing system for biological applications that overcomes much of the length-scale limitations of traditional additive manufacturing printing devices. The instrumentation utilizes two tandem technologies of direct molecular printing and ultraviolet light energy transfer to develop bioengineered devices that are uniform, highly controllable and have resolution and length-scales in the nanometer to micrometer-range. The instrumentation is designed to produce controllable, molecular arrays to investigate receptor-ligand, protein-protein, or enzyme-inhibitor interactions with specific applications to: 1) tissue engineering, 2) cell-based studies that influence cellular differentiation and function, 3) drug discovery and 4) microbiology. The primary goal of the instrumentation is to fabricate novel bioengineered devices that consist of arrays of peptides, glycoproteins or oligonucleotides; precisely defined microfluidic channels; and chemically or geometrically tailored biofunctional chips to advance the research domains listed above that cover VA-funded research at the Southern Arizona VA Health Care System (SAVAHCS). Additionally, the 3D nano-printing system may be accessed by investigators at the academic affiliate, the University of Arizona, and other collaborators throughout the region, which will expand the impact of the instrumentation and enhance research collaborations, specifically between the two nearby medical centers and generally throughout the southwestern region.