权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The Effects of Sex Hormones on Chlamydia Infection

性激素对衣原体感染的影响

基本信息

批准号：
10190235
负责人：
ISABELLE DERRE
金额：
$ 20.26万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-20 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10190235
关键词：
3-Dimensional Address Adverse effects Affect Antibiotics Bacteria Bacterial Infections Biological Biological Models Biology Biomedical Engineering Biomimetics Cell Culture Techniques Cells Characteristics Chlamydia Chlamydia Infections Chlamydia trachomatis Collaborations Cytosol Ectopic Pregnancy Endocrine Environment Epithelial Epithelial Cells Equilibrium Goals Gonadal Steroid Hormones Growth Homeostasis Human Human Microbiome Immune Immune response Immunity Individual Infection Infertility Innate Immune Response Intervention Invaded Knowledge Left Membrane Menstrual cycle Modeling Mucous Membrane Mucous body substance Mus Outcome Pathology Pelvic Inflammatory Disease Phase Physiological Physiology Predisposition Process Production Progesterone Reproductive Health Resistance to infection Role Sexual Transmission Sexually Transmitted Diseases Site Stromal Cells Structure Testing Tight Junctions Time Tissues Vaccines Vagina Woman antimicrobial peptide cell type cervicovaginal chemokine cost effective cytokine epidemiology study neutrophil novel therapeutics pathogen prevent reconstitution reproductive tract response trafficking transmission process vaccine access vaginal microbiota

项目摘要

PROJECT SUMMARY Chlamydia trachomatis is the leading cause of sexually transmitted infections of bacterial origin worldwide. No vaccines are available, infections are often asymptomatic, and if left untreated, long-term sequelae have an adverse effect on women reproductive health. The primary site of infection is the endocervical epithelium, where the bacteria replicates in the host cell cytosol within a membrane-bound compartment called the inclusion. Interventions to prevent the establishment of the Chlamydia replicative niche would reduce vaginal shedding (i.e. transmission) and ascension to the upper reproductive tract (i.e. long-term pathology). It is more and more appreciated that the state of the tissue at the time of infection most likely dictates the outcome of infection; however, little is known about intrinsic and extrinsic factors that collectively prime individual cells within the tissue. Human epidemiological studies, murine and cell culture models of infection, have been used to gain a better understanding of these processes; however, there are substantial limitations with these models. To address this gap, in close collaboration with the Biomimetic Models Core, we will use a human three-dimensional biomimetic cervicovaginal model of chlamydial infection and investigate the effects of sex hormones on C. trachomatis infection. We will assess bacterial growth (Aim 1), tissue integrity and physiology (Aim 2) and the immune response (Aim 3). Altogether, the exposure of ex vivo reconstituted cervicovaginal tissue in its physiological environment to infectious chlamydiae will offer a unique model system to characterize if and how sex hormones influence susceptibility to C. trachomatis infection. In the long term, this model will be applicable to the study of fundamental processes related to any sexually transmitted pathogen and will offer a relatively simple and cost- effective way for testing novel therapies to control the spread of sexually transmitted infections.

项目总结