Methods for determination of glycoprotein glycosylation similarities among disease states
Methods for determination of glycoprotein glycosylation similarities among disease states
批准号:
10194553
负责人:
JOSEPH ZAIA
金额:
$42.08万
依托单位国家:
美国
项目类别:
财政年份:
2019
资助国家:
美国
项目状态:
已结题
起止时间:
2019-09-01 至 2022-12-31
关键词:
AddressAlgorithmsAtherosclerosisAutoimmune DiseasesBindingBioinformaticsBiologicalBiological ProcessBiomedical ResearchBrainBrain regionCSPG3 geneCell Surface ProteinsCell surfaceCellsChondroitin Sulfate ProteoglycanCollagenComplexCore ProteinDataData SetDiseaseDissociationElectron TransportEnvironmentEnzymesExtracellular MatrixFamilyFunctional disorderGenesGlycopeptidesGlycoproteinsGrowth FactorGrowth Factor ReceptorsHeartHeparitin SulfateIntelligenceInterventionIonsKnowledgeLectinLiquid ChromatographyMachine LearningMalignant NeoplasmsMechanicsMediatingMethodsMolecularMorphogenesisNational Institute of General Medical SciencesNeurodegenerative DisordersPathway interactionsPeptidesPhysiologicalPolysaccharidesProcessProtein GlycosylationProteinsProteoglycanProteomicsReceptor Protein-Tyrosine KinasesRegulationReproducibilityRoleSamplingSignal PathwaySiteStructureTechnologyTissuesaggrecanbioinformatics toolbrevicancell growthdata acquisitiondata to knowledgeextracellulargene productglycoprotein structureglycoproteomicsglycosylationhuman diseasehydrophilicityneuropsychiatric disorderpathogenphysical propertyrapid techniquereceptorstatisticstechnology developmenttechnology research and developmentversican
中文摘要
摘要
英文摘要
Abstract
This application addresses NIGMS PAR-17-045 “Focused Technology Research and Development (R01)”.
This initiative supports projects that focus solely on development of technologies with the potential to enable
biomedical research.
Dysregulation of the cellular microenvironment occurs in cancers, neurodevelopmental and neuropsychiatric
diseases. Known as the matrisome, the set of extracellular matrix and cell surface molecules control the
availability of growth factors to cellular receptors and the mechanical-physical properties of the cell
microenvironment. Currently, the limited understanding of regulation of matrisome glycosylation hinders
understanding of the roles of glycosylation-dependent matrisome networks in the basic mechanisms necessary
for targeted intervention of many diseases.
Matrisome function depends on networks of interaction among glycosylated proteins and glycan-binding
lectins. It is not possible using present proteomics and glycoproteomics methods to compare using rigorous
statistics similarities of glycoproteins that differ by disease-related changes in site-specific glycosylation. We
propose to develop technologies to meet this need. Present proteomics methods quantify proteins using a
few representative peptides per gene product; sequence coverage for most proteins is low. Such low
sequence coverage does not suffice to reconstruct the predominant glycosylated proteoforms active in a
biological context. We propose to develop technologies to compare glycoprotein similarities among biological
sample sets. To do this, we will develop MS acquisition and bioinformatics methods for rapid, sensitive and
reproducible mapping of glycoprotein glycosylation to enable statistically rigorous comparison of glycoprotein
similarities. By making these technologies available, we will enable a new level of understanding of the roles
of matrisome networks in human diseases.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
DOI:
10.1021/acs.analchem.1c03543
发表时间:
2022-02-08
期刊:
Analytical chemistry
影响因子:
7.4
作者:
[Cavallero GJ, Zaia J]
通讯作者:
Zaia J
Methods for measuring matrisome molecule similarity during disease processes
-
批准号:10582128
-
项目类别:
-
资助金额:$16.5万
-
财政年份:2022
-
负责人:JOSEPH ZAIA
-
依托单位:
Methods for measuring matrisome molecule similarity during disease processes
-
批准号:10580774
-
项目类别:
-
资助金额:$41.25万
-
财政年份:2022
-
负责人:JOSEPH ZAIA
-
依托单位:
Methods for measuring matrisome molecule similarity during disease processes
-
批准号:10330789
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项目类别:
-
资助金额:$27.23万
-
财政年份:2022
-
负责人:JOSEPH ZAIA
-
依托单位:
An open-source software suite for processing glycomics and glycoproteomics mass spectral data
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批准号:9391486
-
项目类别:
-
资助金额:$41.5万
-
财政年份:2017
-
负责人:JOSEPH ZAIA
-
依托单位:
A Thermo-Fisher Scientific Q-Exactive HF Mass Spectrometry System
-
批准号:9075665
-
项目类别:
-
资助金额:$57.98万
-
财政年份:2016
-
负责人:JOSEPH ZAIA
-
依托单位:
Software for automated interpretation of heparan sulfate tandem mass spectra
-
批准号:9337106
-
项目类别:
-
资助金额:$11.61万
-
财政年份:2015
-
负责人:JOSEPH ZAIA
-
依托单位:
Software for automated interpretation of heparan sulfate tandem mass spectra
-
批准号:9144851
-
项目类别:
-
资助金额:$31.56万
-
财政年份:2015
-
负责人:JOSEPH ZAIA
-
依托单位:
Software for automated interpretation of heparan sulfate tandem mass spectra
-
批准号:8984998
-
项目类别:
-
资助金额:$32.97万
-
财政年份:2015
-
负责人:JOSEPH ZAIA
-
依托单位:
Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays
-
批准号:9079438
-
项目类别:
-
资助金额:$24.92万
-
财政年份:2014
-
负责人:JOSEPH ZAIA
-
依托单位:
Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays
-
批准号:8889224
-
项目类别:
-
资助金额:$24.92万
-
财政年份:2014
-
负责人:JOSEPH ZAIA
-
依托单位:
Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays
-
批准号:8738123
-
项目类别:
-
资助金额:$21.36万
-
财政年份:2014
-
负责人:JOSEPH ZAIA
-
依托单位:
MASS SPECTROMETRY RESOURCE WEB SITE
-
批准号:8365522
-
项目类别:
-
资助金额:$0.31万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
BIOINFORMATICS FOR GLYCOMICS CHALLENGE PROJECT FOR FIRST YEAR GRADUATE STUDENTS
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批准号:8365571
-
项目类别:
-
资助金额:$0.92万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
DIFFERENTIAL CHARACTERIZATION OF GLYCOSAMINOGLYCAN TANDEM MASS SPECTROMETRY DATA
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批准号:8365498
-
项目类别:
-
资助金额:$2.77万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
DEVELOPMENT OF BIOINFORMATICS FOR GLYCOMICS
-
批准号:8365535
-
项目类别:
-
资助金额:$6.15万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
IMPROVED HILIC LC/MS ANALYSIS OF HEPARINOIDS USING A MAKEUP FLOW CHIP
-
批准号:8365549
-
项目类别:
-
资助金额:$2.62万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
STRUCTURAL ANALYSIS OF SULF2 PROCESSING OF EXTRACELLULAR HEPARAN SULFATE
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批准号:8365551
-
项目类别:
-
资助金额:$1.23万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
ANALYSIS OF NITROUS ACID DEPOLYMERIZATION PRODUCTS OF GLYCOSAMINOGLYCANS
-
批准号:8365497
-
项目类别:
-
资助金额:$2.77万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
N-GLYCOSYLATION SITES OF INFLUENZA A VIRUS HEMAGGLUTININ AND SURFACTANT PROTEIN
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批准号:8365550
-
项目类别:
-
资助金额:$5.38万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
AN IMPROVED PLATFORM FOR GAG DISACCHARIDE ANALYSIS USING LC/MS
-
批准号:8365548
-
项目类别:
-
资助金额:$2.31万
-
财政年份:2011
-
负责人:JOSEPH ZAIA
-
依托单位:
海外基金