A Thermo-Fisher Scientific Q-Exactive HF Mass Spectrometry System

 申请者描述(由申请者提供)：本申请是为了满足北大医学院及周边地区12个NIH资助的研究项目对质谱仪的需求。这些由美国国立卫生研究院资助的项目需要最先进的蛋白质组学、糖组学或糖蛋白组学分析能力。美国国立卫生研究院资助的研究人员社区的需求包括：(I)自下而上的交联蛋白和翻译后修饰蛋白的蛋白质组学；(Ii)从组织切片中结合糖组学和蛋白质组学；(Iii)自下而上的糖蛋白组学。在波士顿大学医学院，没有一种仪器能够同时具备这种分析所需的灵敏度、速度和分辨率。因此，目前的要求是用新的Thermo-Fisher Science Q-Exactive HF LC/MS系统取代现有的过时仪器。这种新仪器产生高分辨率、高质量精度的串联质谱图，这对于解释交联蛋白质和翻译后修饰蛋白质是必不可少的。它通过产生高分辨率和高质量精度的目标质谱仪数据来扩展传统的三重四极杆质谱仪的能力，这些数据对于量化生物系统中修饰蛋白质丰度的变化至关重要。它具有独特的串联MS多路传输能力，利用现有的超高性能层析系统。它具有强大的独立于数据的分析功能，包括前体离子多路复用，使修饰多肽的非靶向性定量成为可能。它非常健壮，非常适合高通量分析。 主要用户项目领域包括：(1-3)交叉排列蛋白质或翻译后修饰蛋白质的自下而上的蛋白质组学分析；(4-7)组织组织的糖组学和蛋白质组学的组合；(8、9)自下而上的糖蛋白质组学。总而言之，所要求的仪器系统对于BU医学院校园和周围地区所述的主要和次要用户群体的成功是必要的。

 DESCRIPTION (provided by applicant): This application is to meet the needs of 12 NIH-funded research projects on the BU Medical Campus (BUMC) and surrounding area for mass spectrometry instrumentation. These NIH funded-projects require state-of-the- art proteomics, glycomics or glycoproteomics analysis capabilities. The needs of the community of NIH funded investigators include (i) bottom-up proteomics of cross-linked proteins and post-translationally modified proteins, (ii) combined glycomics and proteomics from histological tissue slides; (iii) bottom-up glycoproteomics. There are no instruments available on the Boston University Medical Campus with the combination of sensitivity, speed and resolution necessary for such analyses. Accordingly, the present request is to replace existing obsolete instrumentation with a new Thermo-Fisher Scientific Q-Exactive HF LC/MS system. This new instrument produces high resolution, high mass accuracy tandem mass spectra that is essential for interpreting cross-linked proteins and post-translationally modified proteins. It extends the capability of traditional triple quadrupole mass spectrometers by producing high resolution and mass accuracy targeted mass spectral data that are crucial for quantifying changes in abundances of modified proteins in biological systems. It has unique tandem MS multiplexing capability that leverages ultra-performance chromatography systems now available. It has powerful data independent analysis features, including precursor ion multiplexing, that enable non-targeted quantification of modified peptides. It is very robust and well-suited to high-throughput analyses. Major user project areas include: (1-3) bottom-up proteomics analysis of cross-lined proteins or post-translationally modified proteins; (4-7) combined glycomics and proteomics of histological tissue; (8, 9) bottom-up glycoproteomics. In summary, the requested instrument system is necessary for the success of the described group of major and minor users on the BU Medical Campus and surrounding area.