Immune Monitoring Core

免疫监测核心

• 批准号: 10197008
• 负责人: Leo Luznik
• 金额: $ 35.47万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-05-21 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10197008
• 关键词: Adverse event; Allogenic; Area; Autologous; Behavior; Biological Assay; Biological Specimen Banks; Blood; Bone Marrow Transplantation; Cells; Clinical Trials; Clonality; Collaborations; Computing Methodologies; Conduct Clinical Trials; Databases; Detection; Disease; Dissection; Effectiveness; Enrollment; Equilibrium; Eragrostis; Expression Profiling; Flow Cytometry; Frequencies; Future; Galactose Binding Lectin; Gene Expression Profile; Genes; Goals; Human; Human Papillomavirus; Image Analysis; Immune; Immune response; Immune system; Immunization; Immunoassay; Immunologic Monitoring; Immunologics; Immunology; Immunology procedure; Immunosuppression; Immunotherapeutic agent; Immunotherapy; Individual; Infrastructure; Ligands; MHC Class I Genes; Measures; Modality; Molecular; Patients; Procedures; Quality Control; RNA; Regulatory T-Lymphocyte; Research; Research Personnel; Research Project Grants; Resources; Signaling Molecule; Specimen; Stains; Standardization; System; T-Lymphocyte; T-cell receptor repertoire; Techniques; Technology; Testing; Therapeutic; Time; Tissues; Tumor Tissue; anti-PD1 therapy; anti-tumor immune response; antigen-specific T cells; base; cancer stem cell; cell type; cytokine; design; digital imaging; human disease; immune function; immune reconstitution; immunogenic; immunoregulation; in vivo; insight; interest; laser capture microdissection; multidimensional data; novel; patient response; peripheral blood; programmed cell death ligand 1; responders and non-responders; response; targeted treatment; therapeutic effectiveness; tumor

Immune Monitoring Core Project Summary The application of immune-based therapies to augment the anti-tumor immune responses following autologous and allogeneic blood or marrow transplantation (BMT) has dramatically increase over the past several years. Reconstitution of the immune system following BMT, GVHD, and post-grafting immunosuppression may modify any immunotherapeutic approach. The need to carefully evaluate, and monitor the immune response in this setting has become increasingly apparent. The principle objective of the Human Immunology Core (Core C) is to serve as the centralized resource for the assessment of immunological monitoring of immune function in patients enrolled on the clinical trials. While there is great diversity in the therapeutic modalities being evaluated, most areas strongly overlap with regard to analysis and characterization of the immune response. A wide-variety of techniques that enumerate and characterize T cells, assess their functional behavior ex vivo, evaluate diversity of the response, characterize their gene expression signature as well as visualize multidimensional data using novel computational methods are available to the research projects within this P01. The specific aims of the Immunology Core (Core C) are to: 1) Provide sophisticated immune monitoring assays for measuring patients’ responses to immune therapies across projects. As a part of this aim, the Human Immunology core (Core C) will provide guidance and expertise to all investigators for the optimal integration of new high- quality correlative assays developed and utilized by the Core C for dissection of immunologic mechanisms, establish standard operating procedures and quality control for immunological assays that will be performed by the project investigators and implement the technical support; 2) Conduct assays and characterize the immune responses for specific clinical trials. As a part of this effort, the Immune Monitoring Core will serve as a repository for biospecimens, maintain the database for designated clinical trials and conduct in-depth mechanistic analysis associated with designated clinical trials conducted under Projects 1-4.

免疫监测核心 项目摘要 基于免疫的疗法在增强自体移植后的抗肿瘤免疫应答中的应用 同种异体血液或骨髓移植（BMT）在过去几年中显著增加。 BMT、GVHD和移植后免疫抑制后免疫系统的重建可能会改变 任何免疫方法。需要仔细评估和监测免疫反应， 背景变得越来越明显。人类免疫学核心（Core C）的主要目标是 作为评估免疫功能的免疫监测的集中资源， 参加临床试验的患者。虽然正在评估的治疗方式多种多样， 大多数领域在免疫反应的分析和表征方面存在很大重叠。种类繁多 计数和表征T细胞的技术，评估其体外功能行为，评估多样性， 的响应，表征他们的基因表达签名，以及可视化多维数据使用 新的计算方法可用于本P01中的研究项目。的具体目标 免疫学核心（核心C）是：1）提供复杂的免疫监测测定， 患者对免疫疗法的反应。作为这一目标的一部分，人类免疫学 核心（核心C）将为所有研究人员提供指导和专业知识，以优化整合新的高- 由Core C开发和使用的质量相关测定用于免疫机制的解剖， 建立免疫学试验的标准操作规程和质量控制， 项目研究人员和实施技术支持; 2）进行分析和表征 用于特定临床试验的免疫反应。作为这项工作的一部分，免疫监测核心将服务于 作为生物样本的储存库，维护指定临床试验的数据库，并进行深入的 与在项目1-4下进行的指定临床试验相关的机制分析。